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Article: Visualization of muscarinic m4 mRNA and M4 receptor subtype in rabbit lung

TitleVisualization of muscarinic m4 mRNA and M4 receptor subtype in rabbit lung
Authors
Issue Date1993
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie
Citation
Life Sciences, 1993, v. 53 n. 19, p. 1501-1508 How to Cite?
AbstractWe have used in situ hybridization to study the localization of muscarinic receptor subtype mRNAs and compared this with the distribution of muscarinic receptor subtypes using receptor autoradiography in tissue sections of the rabbit lung. To localize muscarinic receptor subtype mRNAs, subtype-specific synthetic oligonucleotide probes or riboprobes were generated. Two muscarinic receptor mRNAs (m3 and m4) were detected. The m3 mRNA was expressed in high concentration in tracheal smooth muscle, epithelium and blood vessels and to a lesser extent in bronchiolar smooth muscle while the m4 mRNA was abundantly expressed in bronchiolar smooth muscle and alveolar walls. No expression of m1, m2 or m5 mRNAs was found in any lung structures. This was confirmed by Northern blot analysis which revealed only a single transcript of 3.6 kb for m4 mRNA in rabbit lung. With the exception of the airway epithelium and blood vessels, there was a correspondence between mRNA localization and the distribution of mucarinic receptor subtypes visualized by [3H](-)quinuclidinyl benzilate ([3H]QNB) autoradiographically in the presence of relatively selective muscarinic subtype antagonists (M1-M3) and direct labelling with selective [3H]AF-DX 384. The significance and the reasons for the presence of m4 mRNA and M4 receptors in the rabbit lung are uncertain and warrant further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/162008
ISSN
2015 Impact Factor: 2.685
2015 SCImago Journal Rankings: 1.056
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, JCWen_US
dc.contributor.authorHaddad, EBen_US
dc.contributor.authorBuckley, NJen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:16:36Z-
dc.date.available2012-09-05T05:16:36Z-
dc.date.issued1993en_US
dc.identifier.citationLife Sciences, 1993, v. 53 n. 19, p. 1501-1508en_US
dc.identifier.issn0024-3205en_US
dc.identifier.urihttp://hdl.handle.net/10722/162008-
dc.description.abstractWe have used in situ hybridization to study the localization of muscarinic receptor subtype mRNAs and compared this with the distribution of muscarinic receptor subtypes using receptor autoradiography in tissue sections of the rabbit lung. To localize muscarinic receptor subtype mRNAs, subtype-specific synthetic oligonucleotide probes or riboprobes were generated. Two muscarinic receptor mRNAs (m3 and m4) were detected. The m3 mRNA was expressed in high concentration in tracheal smooth muscle, epithelium and blood vessels and to a lesser extent in bronchiolar smooth muscle while the m4 mRNA was abundantly expressed in bronchiolar smooth muscle and alveolar walls. No expression of m1, m2 or m5 mRNAs was found in any lung structures. This was confirmed by Northern blot analysis which revealed only a single transcript of 3.6 kb for m4 mRNA in rabbit lung. With the exception of the airway epithelium and blood vessels, there was a correspondence between mRNA localization and the distribution of mucarinic receptor subtypes visualized by [3H](-)quinuclidinyl benzilate ([3H]QNB) autoradiographically in the presence of relatively selective muscarinic subtype antagonists (M1-M3) and direct labelling with selective [3H]AF-DX 384. The significance and the reasons for the presence of m4 mRNA and M4 receptors in the rabbit lung are uncertain and warrant further investigation.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescieen_US
dc.relation.ispartofLife Sciencesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLung - Chemistryen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRabbitsen_US
dc.subject.meshReceptors, Muscarinic - Analysis - Geneticsen_US
dc.titleVisualization of muscarinic m4 mRNA and M4 receptor subtype in rabbit lungen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0024-3205(93)90624-C-
dc.identifier.pmid8412514-
dc.identifier.scopuseid_2-s2.0-0027515566en_US
dc.identifier.volume53en_US
dc.identifier.issue19en_US
dc.identifier.spage1501en_US
dc.identifier.epage1508en_US
dc.identifier.isiWOS:A1993MA45100007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridHaddad, EB=7102803008en_US
dc.identifier.scopusauthoridBuckley, NJ=7102676785en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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