File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Differential down-regulation of pulmonary β1- and β2-adrenoceptor messenger RNA with prolonged in vivo infusion of isoprenaline

TitleDifferential down-regulation of pulmonary β1- and β2-adrenoceptor messenger RNA with prolonged in vivo infusion of isoprenaline
Authors
Issue Date1993
Citation
European Journal Of Pharmacology - Molecular Pharmacology Section, 1993, v. 247 n. 2, p. 131-138 How to Cite?
AbstractThe down-regulation by isoprenaline of β1 and β2-adrenoceptors in rat lung was investigated at the receptor protein and messenger RNA level. Rats were treated with either isoprenaline or vehicle for 2 h, 1 day and 7 days. Isoprenaline treatment resulted in significant decreases of both β1- and β2-adrenoceptor density after 1 day with maximal decreases of 65 ± 7 and 65 ± 5% for β1- and β2-adrenoceptors, respectively, at 7 days. The administration of isoprenaline had no effect on binding affinities of either β1 or β2-adrenoceptors. β1-Adrenoceptor mRNA was significantly decreased by 58 ± 10, 73 ± 4 and 51 ± 11% at 2 h, 1 day and 7 days, respectively, after isoprenaline treatment. However, the β2-adrenoceptor mRNA was not changed at 2 h after treatment and was significantly decreased by 35 ± 11 and 45 ± 12% at 1 day and 7 days, respectively after treatment. This time course of β2-adrenoceptor mRNA correlated well with that of the transcription factor cyclic AMP response element binding protein-like DNA binding activity, determined by gel shift assay. These findings indicate the existence of distinct mechanisms for down-regulation of β1- and β2-adrenoceptors and suggest the involvement of cyclic AMP response element binding protein in the down-regulation of β2-arenoceptors in rat lung.
Persistent Identifierhttp://hdl.handle.net/10722/161999
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNishikawa, Men_US
dc.contributor.authorMak, JCWen_US
dc.contributor.authorShirasaki, Hen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:16:33Z-
dc.date.available2012-09-05T05:16:33Z-
dc.date.issued1993en_US
dc.identifier.citationEuropean Journal Of Pharmacology - Molecular Pharmacology Section, 1993, v. 247 n. 2, p. 131-138en_US
dc.identifier.issn0922-4106en_US
dc.identifier.urihttp://hdl.handle.net/10722/161999-
dc.description.abstractThe down-regulation by isoprenaline of β1 and β2-adrenoceptors in rat lung was investigated at the receptor protein and messenger RNA level. Rats were treated with either isoprenaline or vehicle for 2 h, 1 day and 7 days. Isoprenaline treatment resulted in significant decreases of both β1- and β2-adrenoceptor density after 1 day with maximal decreases of 65 ± 7 and 65 ± 5% for β1- and β2-adrenoceptors, respectively, at 7 days. The administration of isoprenaline had no effect on binding affinities of either β1 or β2-adrenoceptors. β1-Adrenoceptor mRNA was significantly decreased by 58 ± 10, 73 ± 4 and 51 ± 11% at 2 h, 1 day and 7 days, respectively, after isoprenaline treatment. However, the β2-adrenoceptor mRNA was not changed at 2 h after treatment and was significantly decreased by 35 ± 11 and 45 ± 12% at 1 day and 7 days, respectively after treatment. This time course of β2-adrenoceptor mRNA correlated well with that of the transcription factor cyclic AMP response element binding protein-like DNA binding activity, determined by gel shift assay. These findings indicate the existence of distinct mechanisms for down-regulation of β1- and β2-adrenoceptors and suggest the involvement of cyclic AMP response element binding protein in the down-regulation of β2-arenoceptors in rat lung.en_US
dc.languageengen_US
dc.relation.ispartofEuropean Journal of Pharmacology - Molecular Pharmacology Sectionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCyclic Amp - Biosynthesisen_US
dc.subject.meshDown-Regulation - Drug Effectsen_US
dc.subject.meshInfusions, Intravenousen_US
dc.subject.meshIsoproterenol - Administration & Dosage - Pharmacologyen_US
dc.subject.meshLung - Drug Effects - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshReceptors, Adrenergic, Beta-1 - Biosynthesis - Drug Effectsen_US
dc.subject.meshReceptors, Adrenergic, Beta-2 - Biosynthesis - Drug Effectsen_US
dc.subject.meshTranscription Factors - Biosynthesisen_US
dc.titleDifferential down-regulation of pulmonary β1- and β2-adrenoceptor messenger RNA with prolonged in vivo infusion of isoprenalineen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0922-4106(93)90070-Pen_US
dc.identifier.pmid8282002-
dc.identifier.scopuseid_2-s2.0-0027442367en_US
dc.identifier.volume247en_US
dc.identifier.issue2en_US
dc.identifier.spage131en_US
dc.identifier.epage138en_US
dc.identifier.isiWOS:A1993MC84800005-
dc.identifier.scopusauthoridNishikawa, M=7402607361en_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridShirasaki, H=7004586584en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats