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Article: Prenatal quantitation of number of X-chromosomes by slot blot hybridization and autoradiography

TitlePrenatal quantitation of number of X-chromosomes by slot blot hybridization and autoradiography
Authors
Issue Date1993
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijgo
Citation
International Journal Of Gynecology And Obstetrics, 1993, v. 40 n. 2, p. 123-129 How to Cite?
AbstractOBJECTIVE: To study the use of slot blot hybridization as a method of prenatal quantication of the number of X-chromosomes in chorionic villi samples. METHOD: DNA of chorionic villi from fetuses with karyotypes of 46,XY; 45,XO; 47,XXX; 69,XXX and 48,XXXX were extracted, slot blotted and hybridized to the following radioactive probes: β 0.9, FVIII, pY 3.4 and pBLUR. DNA of chorionic villi from five other fetuses with unknown karytypes were similarly blotted and hybridized. Autoradiography with pre-exposed films was carried out and the density of each of the hybridized bands was scanned by a laser densitomer. RESULTS: It was found that with increasing amounts of DNA in the samples, the intensity of the bands hybridized with FVIII and β 0.9 probes increased proportionately. However, the intensity of the bands obtained with the probes pY 3.4 and pBLUR (probes containing multiple repeat sequences) varied little with increasing DNA concentrations. The ratios of radioactivity obtained for the two probes FVIII/β 0.9 were well correlated with the number of X-chromosomes in the samples. Calculations of the FVIII/β 0.9 ratio for the five samples with the unknown karyotypes gave a correct prediction of the number of X-chromosomes in each case. CONCLUSION: Slot blot hybridization can be used as a method of prenatal quantitation of the number of X-chromosomes in chorionic villi samples. The method could be useful for rapid prenatal diagnosis of other numerical chromosomal abnormalities.
Persistent Identifierhttp://hdl.handle.net/10722/161990
ISSN
2014 Impact Factor: 1.537
2014 SCImago Journal Rankings: 0.676
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, FYen_US
dc.contributor.authorChan, Ven_US
dc.contributor.authorChan, TKen_US
dc.date.accessioned2012-09-05T05:16:30Z-
dc.date.available2012-09-05T05:16:30Z-
dc.date.issued1993en_US
dc.identifier.citationInternational Journal Of Gynecology And Obstetrics, 1993, v. 40 n. 2, p. 123-129en_US
dc.identifier.issn0020-7292en_US
dc.identifier.urihttp://hdl.handle.net/10722/161990-
dc.description.abstractOBJECTIVE: To study the use of slot blot hybridization as a method of prenatal quantication of the number of X-chromosomes in chorionic villi samples. METHOD: DNA of chorionic villi from fetuses with karyotypes of 46,XY; 45,XO; 47,XXX; 69,XXX and 48,XXXX were extracted, slot blotted and hybridized to the following radioactive probes: β 0.9, FVIII, pY 3.4 and pBLUR. DNA of chorionic villi from five other fetuses with unknown karytypes were similarly blotted and hybridized. Autoradiography with pre-exposed films was carried out and the density of each of the hybridized bands was scanned by a laser densitomer. RESULTS: It was found that with increasing amounts of DNA in the samples, the intensity of the bands hybridized with FVIII and β 0.9 probes increased proportionately. However, the intensity of the bands obtained with the probes pY 3.4 and pBLUR (probes containing multiple repeat sequences) varied little with increasing DNA concentrations. The ratios of radioactivity obtained for the two probes FVIII/β 0.9 were well correlated with the number of X-chromosomes in the samples. Calculations of the FVIII/β 0.9 ratio for the five samples with the unknown karyotypes gave a correct prediction of the number of X-chromosomes in each case. CONCLUSION: Slot blot hybridization can be used as a method of prenatal quantitation of the number of X-chromosomes in chorionic villi samples. The method could be useful for rapid prenatal diagnosis of other numerical chromosomal abnormalities.en_US
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijgoen_US
dc.relation.ispartofInternational Journal of Gynecology and Obstetricsen_US
dc.subject.meshAdulten_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshChorionic Villi Samplingen_US
dc.subject.meshDna Probesen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaternal Ageen_US
dc.subject.meshMolecular Probe Techniquesen_US
dc.subject.meshPregnancyen_US
dc.subject.meshPregnancy, High-Risken_US
dc.subject.meshSex Chromosome Aberrations - Diagnosisen_US
dc.subject.meshX Chromosomeen_US
dc.titlePrenatal quantitation of number of X-chromosomes by slot blot hybridization and autoradiographyen_US
dc.typeArticleen_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0020-7292(93)90371-3en_US
dc.identifier.pmid8094681en_US
dc.identifier.scopuseid_2-s2.0-0027403031en_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage123en_US
dc.identifier.epage129en_US
dc.identifier.isiWOS:A1993KN42300006-
dc.publisher.placeIrelanden_US
dc.identifier.scopusauthoridChan, FY=7202586500en_US
dc.identifier.scopusauthoridChan, V=7202654865en_US
dc.identifier.scopusauthoridChan, TK=7402687762en_US

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