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Article: Prenatal quantitation of number of X-chromosomes by slot blot hybridization and autoradiography
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TitlePrenatal quantitation of number of X-chromosomes by slot blot hybridization and autoradiography
 
AuthorsChan, FY1
Chan, V1
Chan, TK1
 
Issue Date1993
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijgo
 
CitationInternational Journal Of Gynecology And Obstetrics, 1993, v. 40 n. 2, p. 123-129 [How to Cite?]
DOI: http://dx.doi.org/10.1016/0020-7292(93)90371-3
 
AbstractOBJECTIVE: To study the use of slot blot hybridization as a method of prenatal quantication of the number of X-chromosomes in chorionic villi samples. METHOD: DNA of chorionic villi from fetuses with karyotypes of 46,XY; 45,XO; 47,XXX; 69,XXX and 48,XXXX were extracted, slot blotted and hybridized to the following radioactive probes: β 0.9, FVIII, pY 3.4 and pBLUR. DNA of chorionic villi from five other fetuses with unknown karytypes were similarly blotted and hybridized. Autoradiography with pre-exposed films was carried out and the density of each of the hybridized bands was scanned by a laser densitomer. RESULTS: It was found that with increasing amounts of DNA in the samples, the intensity of the bands hybridized with FVIII and β 0.9 probes increased proportionately. However, the intensity of the bands obtained with the probes pY 3.4 and pBLUR (probes containing multiple repeat sequences) varied little with increasing DNA concentrations. The ratios of radioactivity obtained for the two probes FVIII/β 0.9 were well correlated with the number of X-chromosomes in the samples. Calculations of the FVIII/β 0.9 ratio for the five samples with the unknown karyotypes gave a correct prediction of the number of X-chromosomes in each case. CONCLUSION: Slot blot hybridization can be used as a method of prenatal quantitation of the number of X-chromosomes in chorionic villi samples. The method could be useful for rapid prenatal diagnosis of other numerical chromosomal abnormalities.
 
ISSN0020-7292
2013 Impact Factor: 1.563
2013 SCImago Journal Rankings: 0.726
 
DOIhttp://dx.doi.org/10.1016/0020-7292(93)90371-3
 
ISI Accession Number IDWOS:A1993KN42300006
 
DC FieldValue
dc.contributor.authorChan, FY
 
dc.contributor.authorChan, V
 
dc.contributor.authorChan, TK
 
dc.date.accessioned2012-09-05T05:16:30Z
 
dc.date.available2012-09-05T05:16:30Z
 
dc.date.issued1993
 
dc.description.abstractOBJECTIVE: To study the use of slot blot hybridization as a method of prenatal quantication of the number of X-chromosomes in chorionic villi samples. METHOD: DNA of chorionic villi from fetuses with karyotypes of 46,XY; 45,XO; 47,XXX; 69,XXX and 48,XXXX were extracted, slot blotted and hybridized to the following radioactive probes: β 0.9, FVIII, pY 3.4 and pBLUR. DNA of chorionic villi from five other fetuses with unknown karytypes were similarly blotted and hybridized. Autoradiography with pre-exposed films was carried out and the density of each of the hybridized bands was scanned by a laser densitomer. RESULTS: It was found that with increasing amounts of DNA in the samples, the intensity of the bands hybridized with FVIII and β 0.9 probes increased proportionately. However, the intensity of the bands obtained with the probes pY 3.4 and pBLUR (probes containing multiple repeat sequences) varied little with increasing DNA concentrations. The ratios of radioactivity obtained for the two probes FVIII/β 0.9 were well correlated with the number of X-chromosomes in the samples. Calculations of the FVIII/β 0.9 ratio for the five samples with the unknown karyotypes gave a correct prediction of the number of X-chromosomes in each case. CONCLUSION: Slot blot hybridization can be used as a method of prenatal quantitation of the number of X-chromosomes in chorionic villi samples. The method could be useful for rapid prenatal diagnosis of other numerical chromosomal abnormalities.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationInternational Journal Of Gynecology And Obstetrics, 1993, v. 40 n. 2, p. 123-129 [How to Cite?]
DOI: http://dx.doi.org/10.1016/0020-7292(93)90371-3
 
dc.identifier.doihttp://dx.doi.org/10.1016/0020-7292(93)90371-3
 
dc.identifier.epage129
 
dc.identifier.isiWOS:A1993KN42300006
 
dc.identifier.issn0020-7292
2013 Impact Factor: 1.563
2013 SCImago Journal Rankings: 0.726
 
dc.identifier.issue2
 
dc.identifier.pmid8094681
 
dc.identifier.scopuseid_2-s2.0-0027403031
 
dc.identifier.spage123
 
dc.identifier.urihttp://hdl.handle.net/10722/161990
 
dc.identifier.volume40
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/ijgo
 
dc.publisher.placeIreland
 
dc.relation.ispartofInternational Journal of Gynecology and Obstetrics
 
dc.subject.meshAdult
 
dc.subject.meshAutoradiography
 
dc.subject.meshChorionic Villi Sampling
 
dc.subject.meshDna Probes
 
dc.subject.meshFemale
 
dc.subject.meshHumans
 
dc.subject.meshMaternal Age
 
dc.subject.meshMolecular Probe Techniques
 
dc.subject.meshPregnancy
 
dc.subject.meshPregnancy, High-Risk
 
dc.subject.meshSex Chromosome Aberrations - Diagnosis
 
dc.subject.meshX Chromosome
 
dc.titlePrenatal quantitation of number of X-chromosomes by slot blot hybridization and autoradiography
 
dc.typeArticle
 
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<contributor.author>Chan, V</contributor.author>
<contributor.author>Chan, TK</contributor.author>
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<identifier.issn>0020-7292</identifier.issn>
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<description.abstract>OBJECTIVE: To study the use of slot blot hybridization as a method of prenatal quantication of the number of X-chromosomes in chorionic villi samples. METHOD: DNA of chorionic villi from fetuses with karyotypes of 46,XY; 45,XO; 47,XXX; 69,XXX and 48,XXXX were extracted, slot blotted and hybridized to the following radioactive probes: &#946; 0.9, FVIII, pY 3.4 and pBLUR. DNA of chorionic villi from five other fetuses with unknown karytypes were similarly blotted and hybridized. Autoradiography with pre-exposed films was carried out and the density of each of the hybridized bands was scanned by a laser densitomer. RESULTS: It was found that with increasing amounts of DNA in the samples, the intensity of the bands hybridized with FVIII and &#946; 0.9 probes increased proportionately. However, the intensity of the bands obtained with the probes pY 3.4 and pBLUR (probes containing multiple repeat sequences) varied little with increasing DNA concentrations. The ratios of radioactivity obtained for the two probes FVIII/&#946; 0.9 were well correlated with the number of X-chromosomes in the samples. Calculations of the FVIII/&#946; 0.9 ratio for the five samples with the unknown karyotypes gave a correct prediction of the number of X-chromosomes in each case. CONCLUSION: Slot blot hybridization can be used as a method of prenatal quantitation of the number of X-chromosomes in chorionic villi samples. The method could be useful for rapid prenatal diagnosis of other numerical chromosomal abnormalities.</description.abstract>
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<subject.mesh>Adult</subject.mesh>
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<subject.mesh>Female</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Maternal Age</subject.mesh>
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Author Affiliations
  1. Queen Mary Hospital Hong Kong