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Article: ATP stimulates Ca 2+ release from a rapidly exchanging pool in cultured rat epididymal cells

TitleATP stimulates Ca 2+ release from a rapidly exchanging pool in cultured rat epididymal cells
Authors
KeywordsEpithelia
Fura-2 Acetoxymethyl Ester
Microfluorimetry
Short-Circuit Current
Single Cells
Issue Date1993
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1993, v. 264 n. 6 33-6, p. C1388-C1394 How to Cite?
AbstractA study was carried out to investigate an ATP sensitive Ca 2+ pool in rat epididymal cells and its role in transepithelial Cl - secretion. In normal buffered solution containing 2.5 mM free Ca 2+, ATP triggered single calcium spikes in a dose-dependent fashion. In nominally Ca 2+-free solution, the peaks of successive Ca 2+ spikes diminished after repeated ATP stimulations. Addition of Sr 2+ (2.5 mM) to Ca 2+-free solution after ATP stimulation did not cause changes in fluorescence signals. However, in the presence of Sr 2+, ATP gave rise to apparent repetitive Ca 2+ spikes of similar magnitudes after repeated stimulations. Increasing the time of exposure in Ca 2+-free solution containing 50 μM ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid rapidly decreased the intracellular Ca 2+ concentration ([Ca 2+](i)) response to subsequent ATP stimulation. On the other hand, increasing the time of exposure in Sr 2+-containing solution in Ca 2+-depleted cells rapidly increased the apparent [Ca 2+](i) response to subsequent ATP stimulation. These observations suggested the existence of a Ca 2+ pool that was rapidly exchanging with the extracellular compartment. Apical application of ATP elicited a transient rise in short-circuit current across the epididymal epithelium in a dose-dependent fashion, and the response was reduced by prior stimulation with thapsigargin. Ca 2+ released from a rapidly exchanging ATP-sensitive store might stimulate Cl - secretion in the epididymis, thereby maintaining the electrolyte contents and fluidity of the epididymal microenvironment.
Persistent Identifierhttp://hdl.handle.net/10722/161982
ISSN
1998 Impact Factor: 3.077
2004 SCImago Journal Rankings: 1.102

 

DC FieldValueLanguage
dc.contributor.authorLeung, AYHen_US
dc.contributor.authorTai, HLen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-09-05T05:16:27Z-
dc.date.available2012-09-05T05:16:27Z-
dc.date.issued1993en_US
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1993, v. 264 n. 6 33-6, p. C1388-C1394en_US
dc.identifier.issn0002-9513en_US
dc.identifier.urihttp://hdl.handle.net/10722/161982-
dc.description.abstractA study was carried out to investigate an ATP sensitive Ca 2+ pool in rat epididymal cells and its role in transepithelial Cl - secretion. In normal buffered solution containing 2.5 mM free Ca 2+, ATP triggered single calcium spikes in a dose-dependent fashion. In nominally Ca 2+-free solution, the peaks of successive Ca 2+ spikes diminished after repeated ATP stimulations. Addition of Sr 2+ (2.5 mM) to Ca 2+-free solution after ATP stimulation did not cause changes in fluorescence signals. However, in the presence of Sr 2+, ATP gave rise to apparent repetitive Ca 2+ spikes of similar magnitudes after repeated stimulations. Increasing the time of exposure in Ca 2+-free solution containing 50 μM ethylene glycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid rapidly decreased the intracellular Ca 2+ concentration ([Ca 2+](i)) response to subsequent ATP stimulation. On the other hand, increasing the time of exposure in Sr 2+-containing solution in Ca 2+-depleted cells rapidly increased the apparent [Ca 2+](i) response to subsequent ATP stimulation. These observations suggested the existence of a Ca 2+ pool that was rapidly exchanging with the extracellular compartment. Apical application of ATP elicited a transient rise in short-circuit current across the epididymal epithelium in a dose-dependent fashion, and the response was reduced by prior stimulation with thapsigargin. Ca 2+ released from a rapidly exchanging ATP-sensitive store might stimulate Cl - secretion in the epididymis, thereby maintaining the electrolyte contents and fluidity of the epididymal microenvironment.en_US
dc.languageengen_US
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpcon.physiology.org/en_US
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_US
dc.subjectEpitheliaen_US
dc.subjectFura-2 Acetoxymethyl Esteren_US
dc.subjectMicrofluorimetryen_US
dc.subjectShort-Circuit Currenten_US
dc.subjectSingle Cellsen_US
dc.titleATP stimulates Ca 2+ release from a rapidly exchanging pool in cultured rat epididymal cellsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_US
dc.identifier.authorityLeung, AYH=rp00265en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8333493-
dc.identifier.scopuseid_2-s2.0-0027324356en_US
dc.identifier.volume264en_US
dc.identifier.issue6 33-6en_US
dc.identifier.spageC1388en_US
dc.identifier.epageC1394en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, AYH=7403012668en_US
dc.identifier.scopusauthoridTai, HL=36811497300en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

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