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Article: Detection of immunoglobulin gene rearrangement in lymphoid malignancies of B-cell lineage by seminested polymerase chain reaction gene amplification

TitleDetection of immunoglobulin gene rearrangement in lymphoid malignancies of B-cell lineage by seminested polymerase chain reaction gene amplification
Authors
Issue Date1993
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35105
Citation
American Journal Of Hematology, 1993, v. 43 n. 1, p. 24-28 How to Cite?
AbstractSeminested polymerase chain reaction (PCR) was used to amplify the DNA fragments of the complementarity-determining region 3 of the immunoglobulin (Ig) gene heavy chain from the malignant cell specimens of patients with leukemias and lymphomas of B-cell lineage. Two different pairs of primers were used sequentially. Twenty of the 27 (74%) acute lymphoblastic leukemia (ALL) patients, 14 of 19 (74%) chronic lymphocytic leukemia (CLL) patients and eight of 20 (40%) non-Hodgkin's lymphoma (NHL) patients, who had rearrangement of the Ig gene heavy chain by Southern analysis, were positive by the seminested PCR. False-negative results appeared to occur more commonly in cases of lymphoma. The PCR analysis was also less likely to be positive if one-stage PCR studies with either pair of primers were both negative. The seminested PCR technique was found to have a high sensitivity of detecting malignant cells at the level of 0.02%. The clinical application of this assay needs to be investigated further.
Persistent Identifierhttp://hdl.handle.net/10722/161972
ISSN
2015 Impact Factor: 5.0
2015 SCImago Journal Rankings: 1.761
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiang, Ren_US
dc.contributor.authorChan, Ven_US
dc.contributor.authorChan, TKen_US
dc.contributor.authorWong, Ten_US
dc.contributor.authorChiu, Een_US
dc.contributor.authorLie, Aen_US
dc.contributor.authorTodd, Den_US
dc.date.accessioned2012-09-05T05:16:23Z-
dc.date.available2012-09-05T05:16:23Z-
dc.date.issued1993en_US
dc.identifier.citationAmerican Journal Of Hematology, 1993, v. 43 n. 1, p. 24-28en_US
dc.identifier.issn0361-8609en_US
dc.identifier.urihttp://hdl.handle.net/10722/161972-
dc.description.abstractSeminested polymerase chain reaction (PCR) was used to amplify the DNA fragments of the complementarity-determining region 3 of the immunoglobulin (Ig) gene heavy chain from the malignant cell specimens of patients with leukemias and lymphomas of B-cell lineage. Two different pairs of primers were used sequentially. Twenty of the 27 (74%) acute lymphoblastic leukemia (ALL) patients, 14 of 19 (74%) chronic lymphocytic leukemia (CLL) patients and eight of 20 (40%) non-Hodgkin's lymphoma (NHL) patients, who had rearrangement of the Ig gene heavy chain by Southern analysis, were positive by the seminested PCR. False-negative results appeared to occur more commonly in cases of lymphoma. The PCR analysis was also less likely to be positive if one-stage PCR studies with either pair of primers were both negative. The seminested PCR technique was found to have a high sensitivity of detecting malignant cells at the level of 0.02%. The clinical application of this assay needs to be investigated further.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35105en_US
dc.relation.ispartofAmerican Journal of Hematologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBone Marrow - Pathologyen_US
dc.subject.meshDna, Neoplasmen_US
dc.subject.meshGene Rearrangementen_US
dc.subject.meshGenes, Immunoglobulinen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Heavy Chains - Geneticsen_US
dc.subject.meshLeukemia, Lymphocytic, Chronic, B-Cell - Blood - Genetics - Immunology - Pathologyen_US
dc.subject.meshLymph Nodes - Pathologyen_US
dc.subject.meshLymphoma, Non-Hodgkin - Blood - Genetics - Immunology - Pathologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligodeoxyribonucleotidesen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshPrecursor Cell Lymphoblastic Leukemia-Lymphoma - Blood - Genetics - Immunology - Pathologyen_US
dc.titleDetection of immunoglobulin gene rearrangement in lymphoid malignancies of B-cell lineage by seminested polymerase chain reaction gene amplificationen_US
dc.typeArticleen_US
dc.identifier.emailLiang, R:rliang@hku.hken_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityLiang, R=rp00345en_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ajh.2830430107-
dc.identifier.pmid8317459-
dc.identifier.scopuseid_2-s2.0-0027230190en_US
dc.identifier.volume43en_US
dc.identifier.issue1en_US
dc.identifier.spage24en_US
dc.identifier.epage28en_US
dc.identifier.isiWOS:A1993KY26400006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLiang, R=26643224900en_US
dc.identifier.scopusauthoridChan, V=7202654865en_US
dc.identifier.scopusauthoridChan, TK=7402687762en_US
dc.identifier.scopusauthoridWong, T=7403531434en_US
dc.identifier.scopusauthoridChiu, E=24827833600en_US
dc.identifier.scopusauthoridLie, A=7004510870en_US
dc.identifier.scopusauthoridTodd, D=7201388182en_US

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