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Article: Localization of muscarinic receptor subtype mRNAs in human lung.

TitleLocalization of muscarinic receptor subtype mRNAs in human lung.
Authors
Issue Date1992
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrcmb.atsjournals.org
Citation
American Journal Of Respiratory Cell And Molecular Biology, 1992, v. 7 n. 3, p. 344-348 How to Cite?
Abstractm2 mRNA in airway smooth muscle; m3 mRNA in airway epithelium, airway smooth muscle, and submucosal glands. No detection of m4 and m5 mRNAs was observed in any cellular structures. The presence of various muscarinic receptor subtype mRNAs was confirmed by Northern blot analysis. Only human lung mRNA hybridized to the m1 probe giving a single 3.2 kb transcript. mRNA from the human cultured airway smooth muscle cells gave m2 and m3 hybridization bands of about 6.0 kb and 4.5 kb, respectively, while mRNA from the cultured airway epithelial cells gave only m3 hybridization band of 4.5 kb. With the exception of the airway epithelium, there was a good correlation between the distribution of mRNAs by in situ hybridization and the distribution of receptor subtypes by autoradiographic mapping. These results may have an important clinical implication, and also give rise to further investigation of gene regulation of pulmonary muscarinic receptor subtypes in health and disease.
Persistent Identifierhttp://hdl.handle.net/10722/161956
ISSN
2015 Impact Factor: 4.082
2015 SCImago Journal Rankings: 1.622
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, JCen_US
dc.contributor.authorBaraniuk, JNen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:16:18Z-
dc.date.available2012-09-05T05:16:18Z-
dc.date.issued1992en_US
dc.identifier.citationAmerican Journal Of Respiratory Cell And Molecular Biology, 1992, v. 7 n. 3, p. 344-348en_US
dc.identifier.issn1044-1549en_US
dc.identifier.urihttp://hdl.handle.net/10722/161956-
dc.description.abstractm2 mRNA in airway smooth muscle; m3 mRNA in airway epithelium, airway smooth muscle, and submucosal glands. No detection of m4 and m5 mRNAs was observed in any cellular structures. The presence of various muscarinic receptor subtype mRNAs was confirmed by Northern blot analysis. Only human lung mRNA hybridized to the m1 probe giving a single 3.2 kb transcript. mRNA from the human cultured airway smooth muscle cells gave m2 and m3 hybridization bands of about 6.0 kb and 4.5 kb, respectively, while mRNA from the cultured airway epithelial cells gave only m3 hybridization band of 4.5 kb. With the exception of the airway epithelium, there was a good correlation between the distribution of mRNAs by in situ hybridization and the distribution of receptor subtypes by autoradiographic mapping. These results may have an important clinical implication, and also give rise to further investigation of gene regulation of pulmonary muscarinic receptor subtypes in health and disease.en_US
dc.languageengen_US
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrcmb.atsjournals.orgen_US
dc.relation.ispartofAmerican journal of respiratory cell and molecular biologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshHumansen_US
dc.subject.meshLung - Chemistryen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna, Messenger - Analysis - Geneticsen_US
dc.subject.meshReceptors, Muscarinic - Analysis - Geneticsen_US
dc.titleLocalization of muscarinic receptor subtype mRNAs in human lung.en_US
dc.typeArticleen_US
dc.identifier.emailMak, JC:judymak@hku.hken_US
dc.identifier.authorityMak, JC=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1165/ajrcmb/7.3.344-
dc.identifier.pmid1520495-
dc.identifier.scopuseid_2-s2.0-0026917239en_US
dc.identifier.volume7en_US
dc.identifier.issue3en_US
dc.identifier.spage344en_US
dc.identifier.epage348en_US
dc.identifier.isiWOS:A1992JW36200016-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMak, JC=7103323094en_US
dc.identifier.scopusauthoridBaraniuk, JN=7006822080en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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