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Article: Characterization of cytokine gene expression in CD4 + and CD8 + T cells after activation with phorbol myristate acetate and phytohaemagglutinin

TitleCharacterization of cytokine gene expression in CD4 + and CD8 + T cells after activation with phorbol myristate acetate and phytohaemagglutinin
Authors
KeywordsCD4 + T cells
CD8 + T cells
Cytokine gene
mRNA
Polymerase chain reaction
Reverse transcription
Time-course
Issue Date1992
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEI
Citation
Clinical And Experimental Immunology, 1992, v. 90 n. 1, p. 147-153 How to Cite?
AbstractCytokines are important mediators involved in the development of effector cells and in the regulation of immune responses. The gene expression of these mediators in T cell subset has yet to be fully elucidated. Using sensitive reverse transcription-polymerase chain reaction (RT-PCR), the kinetics of cytokine gene expression in human CD4 + and CD8 + T cells were examined. CD4 + T cells were more readily activated by phorbol myristate acetate (PMA) and phytohaemagglutinin (PHA) than CD8 + T cells in terms of the IL-2 receptor (IL-2R) mRNA expression. Quantitative differences in cytokine gene expression between CD4 + and CD8 + T cells were confirmed and higher levels of cytokine mRNAs were induced in CD4 + than in CD8 + T cells. Early induction of IL-2 mRNA was observed in both T cell subsets. The demonstration of different kinetics of cytokine gene expression illustrates one of the examples of the complexity of immunoregulation. The differential response of cytokine gene expression in different T cell subsets should be taken into consideration when clinical studies in cytokine production by peripheral blood mononuclear cells are interpreted.
Persistent Identifierhttp://hdl.handle.net/10722/161953
ISSN
2015 Impact Factor: 3.148
2015 SCImago Journal Rankings: 1.369
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorLai, CKWen_HK
dc.contributor.authorChui, YLen_HK
dc.contributor.authorHo, RTHen_HK
dc.contributor.authorChan, CHSen_HK
dc.contributor.authorLai, KNen_HK
dc.date.accessioned2012-09-05T05:16:17Z-
dc.date.available2012-09-05T05:16:17Z-
dc.date.issued1992en_HK
dc.identifier.citationClinical And Experimental Immunology, 1992, v. 90 n. 1, p. 147-153en_HK
dc.identifier.issn0009-9104en_HK
dc.identifier.urihttp://hdl.handle.net/10722/161953-
dc.description.abstractCytokines are important mediators involved in the development of effector cells and in the regulation of immune responses. The gene expression of these mediators in T cell subset has yet to be fully elucidated. Using sensitive reverse transcription-polymerase chain reaction (RT-PCR), the kinetics of cytokine gene expression in human CD4 + and CD8 + T cells were examined. CD4 + T cells were more readily activated by phorbol myristate acetate (PMA) and phytohaemagglutinin (PHA) than CD8 + T cells in terms of the IL-2 receptor (IL-2R) mRNA expression. Quantitative differences in cytokine gene expression between CD4 + and CD8 + T cells were confirmed and higher levels of cytokine mRNAs were induced in CD4 + than in CD8 + T cells. Early induction of IL-2 mRNA was observed in both T cell subsets. The demonstration of different kinetics of cytokine gene expression illustrates one of the examples of the complexity of immunoregulation. The differential response of cytokine gene expression in different T cell subsets should be taken into consideration when clinical studies in cytokine production by peripheral blood mononuclear cells are interpreted.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEIen_HK
dc.relation.ispartofClinical and Experimental Immunologyen_HK
dc.subjectCD4 + T cellsen_HK
dc.subjectCD8 + T cellsen_HK
dc.subjectCytokine geneen_HK
dc.subjectmRNAen_HK
dc.subjectPolymerase chain reactionen_HK
dc.subjectReverse transcriptionen_HK
dc.subjectTime-courseen_HK
dc.subject.meshAntigens, Cd8 - Analysisen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCd4-Positive T-Lymphocytes - Physiologyen_US
dc.subject.meshCytokines - Geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOligodeoxyribonucleotides - Chemistryen_US
dc.subject.meshPhytohemagglutinins - Administration & Dosageen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshReceptors, Interleukin-2 - Geneticsen_US
dc.subject.meshT-Lymphocyte Subsets - Physiologyen_US
dc.subject.meshTetradecanoylphorbol Acetate - Pharmacologyen_US
dc.titleCharacterization of cytokine gene expression in CD4 + and CD8 + T cells after activation with phorbol myristate acetate and phytohaemagglutininen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1356669-
dc.identifier.scopuseid_2-s2.0-0026768905en_HK
dc.identifier.volume90en_HK
dc.identifier.issue1en_HK
dc.identifier.spage147en_HK
dc.identifier.epage153en_HK
dc.identifier.isiWOS:A1992JQ86300026-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridLai, CKW=7403086390en_HK
dc.identifier.scopusauthoridChui, YL=7004982375en_HK
dc.identifier.scopusauthoridHo, RTH=8886064800en_HK
dc.identifier.scopusauthoridChan, CHS=16169208100en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK

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