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Article: Further evidence of increased polymorphonuclear cell activity in patients with Raynaud's phenomenon

TitleFurther evidence of increased polymorphonuclear cell activity in patients with Raynaud's phenomenon
Authors
Issue Date1992
PublisherOxford University Press. The Journal's web site is located at http://rheumatology.oxfordjournals.org/
Citation
British Journal Of Rheumatology, 1992, v. 31 n. 6, p. 375-380 How to Cite?
AbstractThe role of polymorphonuclear cells (PMNs) in the pathophysiology of ischaemic vascular disease has been increasingly recognized in recent years. Activated PMNs may physically obstruct blood flow. Subsequent release of reactive oxygen radicals and lytic enzymes lead to continued damage. Previous studies have shown increased white cell activity in patients with secondary Raynaud's syndrome (RS). However, whether this is related to the underlying condition or to digital vasospasm is not clear. Using a more physiological whole blood PMN aggregation assay, we assessed PMN activity 38 patients with severe Raynaud's phenomenon (RP) [16 had systemic sclerosis (SSc) and secondary RS; 22 had RP alone and no other features of a connective tissue disease]. Additionally, plasma levels of malondialdehyde (MDA), an indicator of free radical activity, were measured. Results were compared with those obtained from 56 matched volunteers. In order to assess if changes in PMN activity was directly related to digital vasospasm, patients were asked to record the duration and frequency of their Raynaud's attacks during a 2-week period using a pocket sized diary. Correlation between these clinical variables of Raynaud's severity and white cell activity was assessed. Patients with RP, both with and without SSc, showed a significantly greater fall in single PMN count when compared with control subjects [SSc associated RS 48.2 (41.4-56)% versus control, P = 0.04; RP alone 49.3 (46.8-52.1)% versus control, P = 0.01 (Mann-Whitney U-test)]. MDA levels were significantly higher in both groups of patients when compared with control subjects LSSc associated RS 8.35 (7.35-9.6) μmol/l versus control, P = 0.000006; RP alone 7.5 (6.4-8.8) μmol/l versus control, P = 0.003 (Mann-Whitney U-test)]. There were no significant differences in PMN aggregation and MDA levels between the two groups of patients. There were no significant correlations between PMN aggregation and MDA levels and duration and frequency of Raynaud's attacks in either group of patients. In conclusion, we have shown increased PMN activity in patients with RP alone as well as those with SSc associated RS. Such abnormal PMN function may be related to digital vasospasm and not the underlying connective tissue disease. However, no correlation between PMN activity and Raynaud's severity was found. A follow-up study is being planned.
Persistent Identifierhttp://hdl.handle.net/10722/161947
ISSN
2000 Impact Factor: 3.949
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLau, CSen_US
dc.contributor.authorBridges, ABen_US
dc.contributor.authorMuir, Aen_US
dc.contributor.authorScott, Nen_US
dc.contributor.authorBancroft, Aen_US
dc.contributor.authorBelch, JJFen_US
dc.date.accessioned2012-09-05T05:16:13Z-
dc.date.available2012-09-05T05:16:13Z-
dc.date.issued1992en_US
dc.identifier.citationBritish Journal Of Rheumatology, 1992, v. 31 n. 6, p. 375-380en_US
dc.identifier.issn0263-7103en_US
dc.identifier.urihttp://hdl.handle.net/10722/161947-
dc.description.abstractThe role of polymorphonuclear cells (PMNs) in the pathophysiology of ischaemic vascular disease has been increasingly recognized in recent years. Activated PMNs may physically obstruct blood flow. Subsequent release of reactive oxygen radicals and lytic enzymes lead to continued damage. Previous studies have shown increased white cell activity in patients with secondary Raynaud's syndrome (RS). However, whether this is related to the underlying condition or to digital vasospasm is not clear. Using a more physiological whole blood PMN aggregation assay, we assessed PMN activity 38 patients with severe Raynaud's phenomenon (RP) [16 had systemic sclerosis (SSc) and secondary RS; 22 had RP alone and no other features of a connective tissue disease]. Additionally, plasma levels of malondialdehyde (MDA), an indicator of free radical activity, were measured. Results were compared with those obtained from 56 matched volunteers. In order to assess if changes in PMN activity was directly related to digital vasospasm, patients were asked to record the duration and frequency of their Raynaud's attacks during a 2-week period using a pocket sized diary. Correlation between these clinical variables of Raynaud's severity and white cell activity was assessed. Patients with RP, both with and without SSc, showed a significantly greater fall in single PMN count when compared with control subjects [SSc associated RS 48.2 (41.4-56)% versus control, P = 0.04; RP alone 49.3 (46.8-52.1)% versus control, P = 0.01 (Mann-Whitney U-test)]. MDA levels were significantly higher in both groups of patients when compared with control subjects LSSc associated RS 8.35 (7.35-9.6) μmol/l versus control, P = 0.000006; RP alone 7.5 (6.4-8.8) μmol/l versus control, P = 0.003 (Mann-Whitney U-test)]. There were no significant differences in PMN aggregation and MDA levels between the two groups of patients. There were no significant correlations between PMN aggregation and MDA levels and duration and frequency of Raynaud's attacks in either group of patients. In conclusion, we have shown increased PMN activity in patients with RP alone as well as those with SSc associated RS. Such abnormal PMN function may be related to digital vasospasm and not the underlying connective tissue disease. However, no correlation between PMN activity and Raynaud's severity was found. A follow-up study is being planned.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://rheumatology.oxfordjournals.org/en_US
dc.relation.ispartofBritish Journal of Rheumatologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshCell Adhesionen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMalondialdehyde - Blooden_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeutrophils - Cytology - Physiologyen_US
dc.subject.meshRaynaud Disease - Blooden_US
dc.subject.meshScleroderma, Systemic - Blooden_US
dc.subject.meshSeverity Of Illness Indexen_US
dc.titleFurther evidence of increased polymorphonuclear cell activity in patients with Raynaud's phenomenonen_US
dc.typeArticleen_US
dc.identifier.emailLau, CS:cslau@hku.hken_US
dc.identifier.authorityLau, CS=rp01348en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1596699-
dc.identifier.scopuseid_2-s2.0-0026724751en_US
dc.identifier.volume31en_US
dc.identifier.issue6en_US
dc.identifier.spage375en_US
dc.identifier.epage380en_US
dc.identifier.isiWOS:A1992HX27200005-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLau, CS=14035682100en_US
dc.identifier.scopusauthoridBridges, AB=8903578700en_US
dc.identifier.scopusauthoridMuir, A=7103376764en_US
dc.identifier.scopusauthoridScott, N=35451126800en_US
dc.identifier.scopusauthoridBancroft, A=7004599092en_US
dc.identifier.scopusauthoridBelch, JJF=7101752870en_US

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