File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The role of calcitonin gene-related peptide in the regulation of anion secretion by the rat and human epididymis

TitleThe role of calcitonin gene-related peptide in the regulation of anion secretion by the rat and human epididymis
Authors
Issue Date1992
PublisherSociety for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.org
Citation
Journal Of Endocrinology, 1992, v. 133 n. 2, p. 259-268 How to Cite?
AbstractA study was carried out to investigate the role of the calcitonin gene-related peptide (CGRP) in the regulation of electrolyte transport in the rat and human epididymis. In monolayer cultures derived from the rat cauda epididymal cells, CGRP stimulated the short-circuit current (SCC) in a dose-dependent manner with the EC50 (concentration required to produce 50% of the response) at 15 nmol/l. This effect of CGRP was seen when the peptide was added to the basolateral aspect of the cells; apical addition having negligible effect. The CGRP-induced rise in the SCC was dependent on the presence of chloride in the bathing solution. Calcitonin had no effect on the SCC and did not affect the CGRP-induced rise in the SCC. The effect of CGRP on secretion was inhibited in a competitive fashion by the CGRP receptor antagonist CGRP(8-37). In contrast to bradykinin, angiotensin II and endothelin I, the effect of CGRP was independent of prostaglandin synthesis. Measurement of intracellular adenosine 3':5'-cyclic monophosphate showed a time- and dose-dependent increase upon stimulation with CGRP. CGRP also stimulated the SCC in monolayers grown from the human epididymis. The current could be inhibited by apical application of the chloride channel blocker, diphenylamine-2-carboxylate. Immunoreactive CGRP was found in the epithelia of rat and human cauda epididymidis. It is suggested that CGRP may regulate the electrolyte and fluid secretion in the epididymis, thereby providing an optimal microenvironment for the maturation and storage of spermatozoa.
Persistent Identifierhttp://hdl.handle.net/10722/161924
ISSN
2015 Impact Factor: 4.498
2015 SCImago Journal Rankings: 1.910
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeung, AYHen_US
dc.contributor.authorLeung, PYen_US
dc.contributor.authorChengChew, SBen_US
dc.contributor.authorWong, PYDen_US
dc.date.accessioned2012-09-05T05:16:04Z-
dc.date.available2012-09-05T05:16:04Z-
dc.date.issued1992en_US
dc.identifier.citationJournal Of Endocrinology, 1992, v. 133 n. 2, p. 259-268en_US
dc.identifier.issn0022-0795en_US
dc.identifier.urihttp://hdl.handle.net/10722/161924-
dc.description.abstractA study was carried out to investigate the role of the calcitonin gene-related peptide (CGRP) in the regulation of electrolyte transport in the rat and human epididymis. In monolayer cultures derived from the rat cauda epididymal cells, CGRP stimulated the short-circuit current (SCC) in a dose-dependent manner with the EC50 (concentration required to produce 50% of the response) at 15 nmol/l. This effect of CGRP was seen when the peptide was added to the basolateral aspect of the cells; apical addition having negligible effect. The CGRP-induced rise in the SCC was dependent on the presence of chloride in the bathing solution. Calcitonin had no effect on the SCC and did not affect the CGRP-induced rise in the SCC. The effect of CGRP on secretion was inhibited in a competitive fashion by the CGRP receptor antagonist CGRP(8-37). In contrast to bradykinin, angiotensin II and endothelin I, the effect of CGRP was independent of prostaglandin synthesis. Measurement of intracellular adenosine 3':5'-cyclic monophosphate showed a time- and dose-dependent increase upon stimulation with CGRP. CGRP also stimulated the SCC in monolayers grown from the human epididymis. The current could be inhibited by apical application of the chloride channel blocker, diphenylamine-2-carboxylate. Immunoreactive CGRP was found in the epithelia of rat and human cauda epididymidis. It is suggested that CGRP may regulate the electrolyte and fluid secretion in the epididymis, thereby providing an optimal microenvironment for the maturation and storage of spermatozoa.en_US
dc.languageengen_US
dc.publisherSociety for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.orgen_US
dc.relation.ispartofJournal of Endocrinologyen_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnions - Metabolismen_US
dc.subject.meshCalcitonin Gene-Related Peptide - Physiologyen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshCyclic Amp - Metabolismen_US
dc.subject.meshElectrophysiologyen_US
dc.subject.meshEpididymis - Metabolismen_US
dc.subject.meshEpithelium - Metabolismen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshMaleen_US
dc.subject.meshProstaglandins - Biosynthesisen_US
dc.subject.meshRatsen_US
dc.titleThe role of calcitonin gene-related peptide in the regulation of anion secretion by the rat and human epididymisen_US
dc.typeArticleen_US
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_US
dc.identifier.authorityLeung, AYH=rp00265en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1677/joe.0.1330259-
dc.identifier.pmid1319450-
dc.identifier.scopuseid_2-s2.0-0026532509en_US
dc.identifier.volume133en_US
dc.identifier.issue2en_US
dc.identifier.spage259en_US
dc.identifier.epage268en_US
dc.identifier.isiWOS:A1992HV66200014-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridLeung, AYH=7403012668en_US
dc.identifier.scopusauthoridLeung, PY=7401749025en_US
dc.identifier.scopusauthoridChengChew, SB=6701358810en_US
dc.identifier.scopusauthoridWong, PYD=7403980262en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats