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Article: Soluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro

TitleSoluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitro
Authors
KeywordsInterleukin 2 peripheral blood
Interleukin 2 receptors
Lymphocyte culture
T lymphocytes
Issue Date1991
Citation
Pathology, 1991, v. 23 n. 3, p. 224-228 How to Cite?
AbstractFollowing activation in vitro, peripheral blood mononuclear cells (PBMC) express cell-associated interleukin-2 receptors (IL2R). The present study was undertaken to define the proportion of T lymphocyte subsets that express the IL2R (CD25 antigen) upon different mitogenic stimulation. Double immunofluorescence staining with different fluorochromes, fluorescein isothiocyanate and phycoethyrin, was applied for identification of IL2R positive cells and individual lymphocyte subset. The exact percentage of individual activated lymphocyte subset bearing IL2R was enumerated by photographic counting. There was paucity of IL2R in freshly isolated, unstimulated peripheral blood, PBMC cultured without mitogen, and cultured B lymphocytes. Following poke-weed mitogen stimulation in vitro, 19% of CD4 (T-helper/inducer) lymphocytes and 14% of CD8 (T-suppressor/cytotoxic) lymphocytes expressed IL2R. Similarly, 25% of CD4 lymphocytes and 19% of CD8 lymphocytes expressed IL2R following phytohemagglutinin stimulation in vitro. Contrary to the reported data of Tac-positive cells in human lymphoid tissues, our study revealed that, upon lectin mitogen stimulation, approximately 55% of IL2R positive PBMC were CD4 lymphocytes, and 45% of them were CD8 lymphocytes. These observations imply the plausible notion that interleukin-2 mediated immune activation of T lymphocytes in PBMC is different from that in local lymphoid organs. It was also demonstrated that the release of soluble IL2R (sIL2R) and IL2 production in supernatant from cultured PBMC varied with different lectin stimulation. A significant correlation was demonstrated between the cellular and soluble IL2R but the production of IL2 from activated mononuclear cells bore no good correlation with either the cellular IL2R expression or the release of sIL2R.
Persistent Identifierhttp://hdl.handle.net/10722/161913
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorLai, FMen_HK
dc.date.accessioned2012-09-05T05:15:59Z-
dc.date.available2012-09-05T05:15:59Z-
dc.date.issued1991en_HK
dc.identifier.citationPathology, 1991, v. 23 n. 3, p. 224-228en_HK
dc.identifier.issn0031-3205en_HK
dc.identifier.urihttp://hdl.handle.net/10722/161913-
dc.description.abstractFollowing activation in vitro, peripheral blood mononuclear cells (PBMC) express cell-associated interleukin-2 receptors (IL2R). The present study was undertaken to define the proportion of T lymphocyte subsets that express the IL2R (CD25 antigen) upon different mitogenic stimulation. Double immunofluorescence staining with different fluorochromes, fluorescein isothiocyanate and phycoethyrin, was applied for identification of IL2R positive cells and individual lymphocyte subset. The exact percentage of individual activated lymphocyte subset bearing IL2R was enumerated by photographic counting. There was paucity of IL2R in freshly isolated, unstimulated peripheral blood, PBMC cultured without mitogen, and cultured B lymphocytes. Following poke-weed mitogen stimulation in vitro, 19% of CD4 (T-helper/inducer) lymphocytes and 14% of CD8 (T-suppressor/cytotoxic) lymphocytes expressed IL2R. Similarly, 25% of CD4 lymphocytes and 19% of CD8 lymphocytes expressed IL2R following phytohemagglutinin stimulation in vitro. Contrary to the reported data of Tac-positive cells in human lymphoid tissues, our study revealed that, upon lectin mitogen stimulation, approximately 55% of IL2R positive PBMC were CD4 lymphocytes, and 45% of them were CD8 lymphocytes. These observations imply the plausible notion that interleukin-2 mediated immune activation of T lymphocytes in PBMC is different from that in local lymphoid organs. It was also demonstrated that the release of soluble IL2R (sIL2R) and IL2 production in supernatant from cultured PBMC varied with different lectin stimulation. A significant correlation was demonstrated between the cellular and soluble IL2R but the production of IL2 from activated mononuclear cells bore no good correlation with either the cellular IL2R expression or the release of sIL2R.en_HK
dc.languageengen_US
dc.relation.ispartofPathologyen_HK
dc.subjectInterleukin 2 peripheral blooden_HK
dc.subjectInterleukin 2 receptorsen_HK
dc.subjectLymphocyte cultureen_HK
dc.subjectT lymphocytesen_HK
dc.subject.meshAdulten_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression - Genetics - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInterleukin-2 - Metabolismen_US
dc.subject.meshLymphocyte Activation - Genetics - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshReceptors, Interleukin-2 - Genetics - Metabolism - Physiologyen_US
dc.subject.meshT-Lymphocytes - Metabolism - Physiology - Ultrastructureen_US
dc.subject.meshT-Lymphocytes, Helper-Inducer - Metabolism - Physiology - Ultrastructureen_US
dc.subject.meshT-Lymphocytes, Regulatory - Metabolism - Physiology - Ultrastructureen_US
dc.titleSoluble interleukin 2 receptor release, interleukin 2 production, and interleukin 2 receptor expression in activated T-lymphocytes in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3109/00313029109063570-
dc.identifier.pmid1838146en_HK
dc.identifier.scopuseid_2-s2.0-0026357062en_HK
dc.identifier.volume23en_HK
dc.identifier.issue3en_HK
dc.identifier.spage224en_HK
dc.identifier.epage228en_HK
dc.identifier.isiWOS:A1991HJ67800009-
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridLai, FM=7202559720en_HK

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