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Article: Autoradiographic visualization of bradykinin receptors in human and guinea pig lung

TitleAutoradiographic visualization of bradykinin receptors in human and guinea pig lung
Authors
Issue Date1991
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
Citation
European Journal Of Pharmacology, 1991, v. 194 n. 1, p. 37-43 How to Cite?
AbstractHigh affinity [3H]bradykinin (BK) receptor binding sites have been identified in human and guinea pig lung sections by in vitro autoradiography. [3H]BK was incubated with tissue sections for 120 min at 25°C and non-specific binding determined by incubating adjacent serial sections in the presence of unlabelled BK. In saturation experiments with guinea pig lung sections, a single class of high affinity binding sites was identified with an apparent dissociation constant (K(d)) of 0.5 ± 0.1 nM and a maximal binding capacity (B(max)) of 35.2 ± 2.9 fmol/mg protein (n = 5). The binding of [3H]BK was inhibited by unlabelled BK and NPC 349 (a specific B2 antagonist) at IC50 of 2.7 ± 0.4 and 87 ± 9 nM (n = 3), respectively. In contrast, no inhibition was found at 1 μM for a variety of vasoactive peptides such substance P, calcitonin gene-related peptide, vasoactive intestinal peptide and des-Arg9-[Leu8]BK (a specific B1-antagonist). Autoradiography revealed that BK receptors were widely distributed in human and guinea pig lung, with dense labelling over bronchial and pulmonary blood vessels of all sizes and in the lamina propria immediately subjacent to the basal epithelial cell layer in large airways. Airway smooth muscle was sparsely labelled in large airways, but greater labelling in smaller airways. There was also detectable labelling over submucosal glands and nerve fibers in human intrapulmonary bronchi and over alveolar walls in both species. The high density of BK receptors on bronchial and pulmonary blood vessels indicate that BK may play an important role in the regulation of airway and pulmonary blood flow, as well as airway epithelial regulation.
Persistent Identifierhttp://hdl.handle.net/10722/161896
ISSN
2015 Impact Factor: 2.73
2015 SCImago Journal Rankings: 1.115
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, JCWen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:15:53Z-
dc.date.available2012-09-05T05:15:53Z-
dc.date.issued1991en_US
dc.identifier.citationEuropean Journal Of Pharmacology, 1991, v. 194 n. 1, p. 37-43en_US
dc.identifier.issn0014-2999en_US
dc.identifier.urihttp://hdl.handle.net/10722/161896-
dc.description.abstractHigh affinity [3H]bradykinin (BK) receptor binding sites have been identified in human and guinea pig lung sections by in vitro autoradiography. [3H]BK was incubated with tissue sections for 120 min at 25°C and non-specific binding determined by incubating adjacent serial sections in the presence of unlabelled BK. In saturation experiments with guinea pig lung sections, a single class of high affinity binding sites was identified with an apparent dissociation constant (K(d)) of 0.5 ± 0.1 nM and a maximal binding capacity (B(max)) of 35.2 ± 2.9 fmol/mg protein (n = 5). The binding of [3H]BK was inhibited by unlabelled BK and NPC 349 (a specific B2 antagonist) at IC50 of 2.7 ± 0.4 and 87 ± 9 nM (n = 3), respectively. In contrast, no inhibition was found at 1 μM for a variety of vasoactive peptides such substance P, calcitonin gene-related peptide, vasoactive intestinal peptide and des-Arg9-[Leu8]BK (a specific B1-antagonist). Autoradiography revealed that BK receptors were widely distributed in human and guinea pig lung, with dense labelling over bronchial and pulmonary blood vessels of all sizes and in the lamina propria immediately subjacent to the basal epithelial cell layer in large airways. Airway smooth muscle was sparsely labelled in large airways, but greater labelling in smaller airways. There was also detectable labelling over submucosal glands and nerve fibers in human intrapulmonary bronchi and over alveolar walls in both species. The high density of BK receptors on bronchial and pulmonary blood vessels indicate that BK may play an important role in the regulation of airway and pulmonary blood flow, as well as airway epithelial regulation.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejpharen_US
dc.relation.ispartofEuropean Journal of Pharmacologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBradykinin - Analogs & Derivatives - Antagonists & Inhibitors - Metabolism - Pharmacologyen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshKineticsen_US
dc.subject.meshLung - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshReceptors, Bradykininen_US
dc.subject.meshReceptors, Neurotransmitter - Metabolismen_US
dc.subject.meshSpecies Specificityen_US
dc.titleAutoradiographic visualization of bradykinin receptors in human and guinea pig lungen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0014-2999(91)90121-6-
dc.identifier.pmid1647963en_US
dc.identifier.scopuseid_2-s2.0-0026021802en_US
dc.identifier.volume194en_US
dc.identifier.issue1en_US
dc.identifier.spage37en_US
dc.identifier.epage43en_US
dc.identifier.isiWOS:A1991FA84700007-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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