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Article: Detection of immunoglobulin gene rearrangement in acute and chronic lymphoid leukemias of B-cell lineage by polymerase chain reaction gene amplification

TitleDetection of immunoglobulin gene rearrangement in acute and chronic lymphoid leukemias of B-cell lineage by polymerase chain reaction gene amplification
Authors
Issue Date1991
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35105
Citation
American Journal Of Hematology, 1991, v. 38 n. 3, p. 189-193 How to Cite?
AbstractPolymerase chain reaction (PCR) was used to amplify the DNA fragments of the complementarity determining region 3 of the immunoglobulin (Ig) gene heavy chain from the leukemic cell specimens of patients with acute and chronic lymphoid leukemias of B-cell lineage. Two different pairs of primers were tested. Fourteen of the 17 (82%) cases of acute lymphoblastic leukemia (ALL), and all 15 cases (100%) of B-cell chronic lymphocytic leukemia, who had rearrangement of the Ig gene heavy chain by Southern analysis, were positive by PCR with either one or both pairs of primers. This technique was able to detect leukemic cells at the level of 0.1%. Applying it to study the remission marrow specimens following induction chemotherapy was more useful than morphology alone in predicting early relapse of the leukemia.
Persistent Identifierhttp://hdl.handle.net/10722/161890
ISSN
2015 Impact Factor: 5.0
2015 SCImago Journal Rankings: 1.761
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiang, Ren_US
dc.contributor.authorChan, Ven_US
dc.contributor.authorChan, TKen_US
dc.contributor.authorWong, Ten_US
dc.contributor.authorChiu, Een_US
dc.contributor.authorTodd, Den_US
dc.date.accessioned2012-09-05T05:15:51Z-
dc.date.available2012-09-05T05:15:51Z-
dc.date.issued1991en_US
dc.identifier.citationAmerican Journal Of Hematology, 1991, v. 38 n. 3, p. 189-193en_US
dc.identifier.issn0361-8609en_US
dc.identifier.urihttp://hdl.handle.net/10722/161890-
dc.description.abstractPolymerase chain reaction (PCR) was used to amplify the DNA fragments of the complementarity determining region 3 of the immunoglobulin (Ig) gene heavy chain from the leukemic cell specimens of patients with acute and chronic lymphoid leukemias of B-cell lineage. Two different pairs of primers were tested. Fourteen of the 17 (82%) cases of acute lymphoblastic leukemia (ALL), and all 15 cases (100%) of B-cell chronic lymphocytic leukemia, who had rearrangement of the Ig gene heavy chain by Southern analysis, were positive by PCR with either one or both pairs of primers. This technique was able to detect leukemic cells at the level of 0.1%. Applying it to study the remission marrow specimens following induction chemotherapy was more useful than morphology alone in predicting early relapse of the leukemia.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35105en_US
dc.relation.ispartofAmerican Journal of Hematologyen_US
dc.subject.meshBurkitt Lymphoma - Geneticsen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshGene Rearrangementen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Variable Region - Geneticsen_US
dc.subject.meshImmunoglobulins - Geneticsen_US
dc.subject.meshImmunophenotypingen_US
dc.subject.meshLeukemia, Lymphocytic, Chronic, B-Cell - Geneticsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.titleDetection of immunoglobulin gene rearrangement in acute and chronic lymphoid leukemias of B-cell lineage by polymerase chain reaction gene amplificationen_US
dc.typeArticleen_US
dc.identifier.emailLiang, R:rliang@hku.hken_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityLiang, R=rp00345en_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ajh.2830380307-
dc.identifier.pmid1951317-
dc.identifier.scopuseid_2-s2.0-0026001123en_US
dc.identifier.volume38en_US
dc.identifier.issue3en_US
dc.identifier.spage189en_US
dc.identifier.epage193en_US
dc.identifier.isiWOS:A1991GL86300006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLiang, R=26643224900en_US
dc.identifier.scopusauthoridChan, V=7202654865en_US
dc.identifier.scopusauthoridChan, TK=7402687762en_US
dc.identifier.scopusauthoridWong, T=7403531434en_US
dc.identifier.scopusauthoridChiu, E=24827833600en_US
dc.identifier.scopusauthoridTodd, D=7201388182en_US

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