File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Localization of β 2-adrenoceptor messenger RNA in human and rat lung using in situ hybridization: Correlation with receptor autoradiography

TitleLocalization of β 2-adrenoceptor messenger RNA in human and rat lung using in situ hybridization: Correlation with receptor autoradiography
Authors
Issue Date1991
Citation
European Journal Of Pharmacology - Molecular Pharmacology Section, 1991, v. 206 n. 2, p. 133-138 How to Cite?
AbstractWe have used in situ hybridization to study the localization of mRNA encoding the β 2-adrenoceptor in tissue sections of the human and rat lung and compared this with the distribution of β 2-receptor binding sites using receptor autoradiography. To localize β 2-receptor mRNA, a [ 32P]labeled antisense RNA probe (riboprobe) was generated from human or rat β 2-receptor cDNA. A similar distribution of β 2-receptor mRNA was identified in both species. The highest intensity of β 2-receptor mRNA was detected in smooth muscle of small airways, airway epithelium and pulmonary blood vessels. Lower intensity of β 2-receptor mRNA was identified in smooth muscle of large airways, and alveolar epithelium (presumably type I and type II pneumocytes). No significant hybridization signal was detected in interstitial tissue. The specificity of the hybridization signal was confirmed with a sense probe (having identical sequence to the mRNA) and preincubation with RNase A, and by Northern blot analysis which revealed a single band of mRNA of 2.2 kb. There was a correspondence between mRNA localization and the distribution of β 2-receptors visualized by [ 125I]iodocyanopindolol autoradiographically in the presence of CGP 20712 (a β 1-selective antagonist). However, alveolar walls that showed a high β 2-receptor density had relatively low levels of mRNA. This cellular heterogeneity may reflect differences in RNA stability or transcription rate in different lung cells. This approach opens up new options in the investigation of the regulation of pulmonary β 2-receptor gene expression in health and disease.
Persistent Identifierhttp://hdl.handle.net/10722/161889
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHamid, QAen_US
dc.contributor.authorMak, JCWen_US
dc.contributor.authorSheppard, MNen_US
dc.contributor.authorCorrin, Ben_US
dc.contributor.authorVenter, JCen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:15:50Z-
dc.date.available2012-09-05T05:15:50Z-
dc.date.issued1991en_US
dc.identifier.citationEuropean Journal Of Pharmacology - Molecular Pharmacology Section, 1991, v. 206 n. 2, p. 133-138en_US
dc.identifier.issn0922-4106en_US
dc.identifier.urihttp://hdl.handle.net/10722/161889-
dc.description.abstractWe have used in situ hybridization to study the localization of mRNA encoding the β 2-adrenoceptor in tissue sections of the human and rat lung and compared this with the distribution of β 2-receptor binding sites using receptor autoradiography. To localize β 2-receptor mRNA, a [ 32P]labeled antisense RNA probe (riboprobe) was generated from human or rat β 2-receptor cDNA. A similar distribution of β 2-receptor mRNA was identified in both species. The highest intensity of β 2-receptor mRNA was detected in smooth muscle of small airways, airway epithelium and pulmonary blood vessels. Lower intensity of β 2-receptor mRNA was identified in smooth muscle of large airways, and alveolar epithelium (presumably type I and type II pneumocytes). No significant hybridization signal was detected in interstitial tissue. The specificity of the hybridization signal was confirmed with a sense probe (having identical sequence to the mRNA) and preincubation with RNase A, and by Northern blot analysis which revealed a single band of mRNA of 2.2 kb. There was a correspondence between mRNA localization and the distribution of β 2-receptors visualized by [ 125I]iodocyanopindolol autoradiographically in the presence of CGP 20712 (a β 1-selective antagonist). However, alveolar walls that showed a high β 2-receptor density had relatively low levels of mRNA. This cellular heterogeneity may reflect differences in RNA stability or transcription rate in different lung cells. This approach opens up new options in the investigation of the regulation of pulmonary β 2-receptor gene expression in health and disease.en_US
dc.languageengen_US
dc.relation.ispartofEuropean Journal of Pharmacology - Molecular Pharmacology Sectionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshHumansen_US
dc.subject.meshLung - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshReceptors, Adrenergic, Beta - Genetics - Metabolismen_US
dc.subject.meshTissue Distributionen_US
dc.titleLocalization of β 2-adrenoceptor messenger RNA in human and rat lung using in situ hybridization: Correlation with receptor autoradiographyen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0922-4106(91)90021-9en_US
dc.identifier.pmid1648500-
dc.identifier.scopuseid_2-s2.0-0025981748en_US
dc.identifier.volume206en_US
dc.identifier.issue2en_US
dc.identifier.spage133en_US
dc.identifier.epage138en_US
dc.identifier.isiWOS:A1991FG90300007-
dc.identifier.scopusauthoridHamid, QA=7102995930en_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridSheppard, MN=7202508828en_US
dc.identifier.scopusauthoridCorrin, B=7004522487en_US
dc.identifier.scopusauthoridVenter, JC=55254617200en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats