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Article: The effects of blockade of interleukin 2 receptors and interleukin 4 receptors on cytokine production

TitleThe effects of blockade of interleukin 2 receptors and interleukin 4 receptors on cytokine production
Authors
Keywordsinterleukin 2
interleukin 4
interleukin 6
peripheral blood lymphocytes
receptor blockade
Issue Date1991
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 1991, v. 99 n. 5, p. 434-442 How to Cite?
AbstractThe understanding of the regulation of cytokine production and T-cell proliferation by other cytokines is far from complete. We have examined the supernatant concentration of different cytokines in cultured peripheral blood lymphocytes (PBL) incubated with monoclonal antibodies against the interleukin-2 receptor (IL-2R) or interleukin-4 receptor (IL-4R) complex in order to analyse the importance of these receptors in the production of other cytokines. The PBL cultures were stimulated with phytohaemagglutinin and phorbol myristate acetate. The addition of anti-Tac to PBL cultures resulted in increased free IL-2 due to blockade of IL-2R. MR6 (a monoclonal antibody against IL-4R) did not affect the supernatant concentration of IL-2 in cultured PBL. Blockade of IL-2R or IL-4R both resulted in increased IL-4 in supernatant. The increase in supernatant concentration of IL-4 in PBL cultured with MR6 was due to a direct interaction with the IL-4R complex but the increase in lL-4 with anti-Tac was due to the accumulation of free IL-4 as an effective blockade of IL-2R prevented the full expression of IL-4R. Neither anti-Tac nor MR6 affected the IL-6 production by activated PBL, suggesting the existence of an IL-2- and IL-4-independent pathway. Blockade of IL-2R or IL-4R both resulted in a reduction of interferon-gamma (IFN-gamma) production, indicating that both endogenously generated IL-2 and IL-4 are important mediators of IFN-gamma induction in PBL cultures stimulated with T-cell mitogens.
Persistent Identifierhttp://hdl.handle.net/10722/161856
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorLi, PKTen_HK
dc.contributor.authorLarche, Men_HK
dc.contributor.authorRitter, MAen_HK
dc.date.accessioned2012-09-05T05:15:33Z-
dc.date.available2012-09-05T05:15:33Z-
dc.date.issued1991en_HK
dc.identifier.citationApmis, 1991, v. 99 n. 5, p. 434-442en_HK
dc.identifier.issn0903-4641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/161856-
dc.description.abstractThe understanding of the regulation of cytokine production and T-cell proliferation by other cytokines is far from complete. We have examined the supernatant concentration of different cytokines in cultured peripheral blood lymphocytes (PBL) incubated with monoclonal antibodies against the interleukin-2 receptor (IL-2R) or interleukin-4 receptor (IL-4R) complex in order to analyse the importance of these receptors in the production of other cytokines. The PBL cultures were stimulated with phytohaemagglutinin and phorbol myristate acetate. The addition of anti-Tac to PBL cultures resulted in increased free IL-2 due to blockade of IL-2R. MR6 (a monoclonal antibody against IL-4R) did not affect the supernatant concentration of IL-2 in cultured PBL. Blockade of IL-2R or IL-4R both resulted in increased IL-4 in supernatant. The increase in supernatant concentration of IL-4 in PBL cultured with MR6 was due to a direct interaction with the IL-4R complex but the increase in lL-4 with anti-Tac was due to the accumulation of free IL-4 as an effective blockade of IL-2R prevented the full expression of IL-4R. Neither anti-Tac nor MR6 affected the IL-6 production by activated PBL, suggesting the existence of an IL-2- and IL-4-independent pathway. Blockade of IL-2R or IL-4R both resulted in a reduction of interferon-gamma (IFN-gamma) production, indicating that both endogenously generated IL-2 and IL-4 are important mediators of IFN-gamma induction in PBL cultures stimulated with T-cell mitogens.en_HK
dc.languageengen_US
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_HK
dc.relation.ispartofAPMISen_HK
dc.subjectinterleukin 2en_HK
dc.subjectinterleukin 4en_HK
dc.subjectinterleukin 6en_HK
dc.subjectperipheral blood lymphocytesen_HK
dc.subjectreceptor blockadeen_HK
dc.subject.meshAntibodies, Monoclonal - Diagnostic Useen_US
dc.subject.meshAntigens, Cd - Analysisen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCulture Techniques - Methodsen_US
dc.subject.meshCytokines - Biosynthesisen_US
dc.subject.meshDna Replicationen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-Gamma - Analysis - Biosynthesisen_US
dc.subject.meshInterleukin-2 - Analysis - Biosynthesisen_US
dc.subject.meshInterleukin-4 - Analysis - Biosynthesisen_US
dc.subject.meshKineticsen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshLymphocytes - Drug Effects - Immunologyen_US
dc.subject.meshReceptors, Interleukin-2 - Analysis - Immunology - Physiologyen_US
dc.subject.meshReceptors, Interleukin-4en_US
dc.subject.meshReceptors, Mitogen - Immunology - Physiologyen_US
dc.titleThe effects of blockade of interleukin 2 receptors and interleukin 4 receptors on cytokine productionen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1699-0463.1991.tb05172.x-
dc.identifier.pmid1904250-
dc.identifier.scopuseid_2-s2.0-0025729660en_HK
dc.identifier.volume99en_HK
dc.identifier.issue5en_HK
dc.identifier.spage434en_HK
dc.identifier.epage442en_HK
dc.identifier.isiWOS:A1991FQ49800006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridLi, PKT=25928016800en_HK
dc.identifier.scopusauthoridLarche, M=7006394560en_HK
dc.identifier.scopusauthoridRitter, MA=7402696170en_HK

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