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Article: Autoradiographic visualization of muscarinic receptor subtypes in human and guinea pig lung

TitleAutoradiographic visualization of muscarinic receptor subtypes in human and guinea pig lung
Authors
Issue Date1990
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org
Citation
American Review Of Respiratory Disease, 1990, v. 141 n. 6, p. 1559-1568 How to Cite?
AbstractMuscarinic receptor subtypes have been localized in human and guinea pig lung sections by an autoradiographic technique, using [3H](-)quinuclidinyl benzilate ([3H]QNB) and selective muscarinic antagonists. [3H]QNB was incubated with tissue sections for 90 min at 25°C, and nonspecific binding was determined by incubating adjacent serial sections in the presence of 1 μM atropine. Binding to lung sections had the characterization expected for muscarinic receptors. Autoradiography revealed that muscarinic receptors were widely distributed in human lung, with dense labeling over submucosal glands and airway ganglia, and moderate labeling over nerves in intrapulmonary bronchi and of airway smooth muscle of large and small airways. In addition, alveolar walls were uniformly labeled. In guinea pig, labeling of airway smooth muscle was similar, but in contrast to human airways, epithelium was labeled but alveolar walls were not. The muscarinic receptors of human airway smooth muscle from large to small airways were entirely of the M3-subtype, whereas in guinea pig airway smooth muscle, the majority were the M3-subtype with a very small population of the M2-subtype present. In human bronchial submucosal glands, M1- and M3-subtypes appeared to coexist in the proportions of 36 and 64%, respectively. In human alveolar walls the muscarinic receptors were entirely of the M1-subtype, which is absent from the guinea pig lung. No M2-receptors were demonstrated in human lung. The localization of M1-receptors was confirmed by direct labeling with [3H]pirenzepine. With the exception of the alveolar walls in human lung, the localization of muscarinic receptor subtypes on structures in the lung is consistent with known functional studies.
Persistent Identifierhttp://hdl.handle.net/10722/161848
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, JCWen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:15:29Z-
dc.date.available2012-09-05T05:15:29Z-
dc.date.issued1990en_US
dc.identifier.citationAmerican Review Of Respiratory Disease, 1990, v. 141 n. 6, p. 1559-1568en_US
dc.identifier.issn0003-0805en_US
dc.identifier.urihttp://hdl.handle.net/10722/161848-
dc.description.abstractMuscarinic receptor subtypes have been localized in human and guinea pig lung sections by an autoradiographic technique, using [3H](-)quinuclidinyl benzilate ([3H]QNB) and selective muscarinic antagonists. [3H]QNB was incubated with tissue sections for 90 min at 25°C, and nonspecific binding was determined by incubating adjacent serial sections in the presence of 1 μM atropine. Binding to lung sections had the characterization expected for muscarinic receptors. Autoradiography revealed that muscarinic receptors were widely distributed in human lung, with dense labeling over submucosal glands and airway ganglia, and moderate labeling over nerves in intrapulmonary bronchi and of airway smooth muscle of large and small airways. In addition, alveolar walls were uniformly labeled. In guinea pig, labeling of airway smooth muscle was similar, but in contrast to human airways, epithelium was labeled but alveolar walls were not. The muscarinic receptors of human airway smooth muscle from large to small airways were entirely of the M3-subtype, whereas in guinea pig airway smooth muscle, the majority were the M3-subtype with a very small population of the M2-subtype present. In human bronchial submucosal glands, M1- and M3-subtypes appeared to coexist in the proportions of 36 and 64%, respectively. In human alveolar walls the muscarinic receptors were entirely of the M1-subtype, which is absent from the guinea pig lung. No M2-receptors were demonstrated in human lung. The localization of M1-receptors was confirmed by direct labeling with [3H]pirenzepine. With the exception of the alveolar walls in human lung, the localization of muscarinic receptor subtypes on structures in the lung is consistent with known functional studies.en_US
dc.languageengen_US
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.orgen_US
dc.relation.ispartofAmerican Review of Respiratory Diseaseen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshDiamines - Diagnostic Useen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshLung - Ultrastructureen_US
dc.subject.meshParasympatholytics - Diagnostic Useen_US
dc.subject.meshPiperidines - Diagnostic Useen_US
dc.subject.meshPirenzepine - Diagnostic Useen_US
dc.subject.meshQuinuclidinyl Benzilate - Diagnostic Useen_US
dc.subject.meshRadioligand Assayen_US
dc.subject.meshReceptors, Muscarinic - Isolation & Purificationen_US
dc.subject.meshTritium - Diagnostic Useen_US
dc.titleAutoradiographic visualization of muscarinic receptor subtypes in human and guinea pig lungen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1164/ajrccm/141.6.1559-
dc.identifier.pmid2350099-
dc.identifier.scopuseid_2-s2.0-0025375882en_US
dc.identifier.volume141en_US
dc.identifier.issue6en_US
dc.identifier.spage1559en_US
dc.identifier.epage1568en_US
dc.identifier.isiWOS:A1990DH87900034-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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