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Article: Peripheral type benzodiazepine receptors in human and guinea pig lung: Characterization and autoradiographic mapping

TitlePeripheral type benzodiazepine receptors in human and guinea pig lung: Characterization and autoradiographic mapping
Authors
Issue Date1990
PublisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org
Citation
Journal Of Pharmacology And Experimental Therapeutics, 1990, v. 252 n. 2, p. 880-885 How to Cite?
AbstractBinding of [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to human and guinea pig lung membranes was characterized and the binding sites localized in lung sections by light microscopic autoradiograpy. Inhibition of [3H]Ro5-4864 binding by unlabeled Ro5-4864, PK11195, midazolam, diazepam, clonazepam and flumazenil indicated that [3H]Ro5-4864 bound to specific receptors and binding was reversible. Scatchard analysis indicated a single class of binding site, with a dissociation constant (K(d)) of 12.8 ± 2.1 nM with maximum binding capacity of 2.6 ± 0.6 pmol/mg of protein for human lung and K(d) of 8.4 ± 1.5 nM and maximum binding capacity of 3.5 ± 0.3 pmol/mg of protein for guinea pig lung. Autoradiograms showed specific labeling over discrete structures in both human and guinea pig lung. Labeling was particularly dense over submucosal glands in intrapulmonary bronchi of human. Airways were also labeled, with epithelium having a higher grain density than smooth muscle. Labeling over smooth muscle was denser in small compared to large airways. Vascular smooth muscle was not labeled in either human and guinea pig. The alveolar walls were uniformly labeled in both species. The functional significance of pulmonary peripheral-type benzodiazepine receptors remains to be determined.
Persistent Identifierhttp://hdl.handle.net/10722/161841
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.847
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, JCWen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:15:25Z-
dc.date.available2012-09-05T05:15:25Z-
dc.date.issued1990en_US
dc.identifier.citationJournal Of Pharmacology And Experimental Therapeutics, 1990, v. 252 n. 2, p. 880-885en_US
dc.identifier.issn0022-3565en_US
dc.identifier.urihttp://hdl.handle.net/10722/161841-
dc.description.abstractBinding of [3H]Ro5-4864, a ligand selective for peripheral-type benzodiazepine receptors, to human and guinea pig lung membranes was characterized and the binding sites localized in lung sections by light microscopic autoradiograpy. Inhibition of [3H]Ro5-4864 binding by unlabeled Ro5-4864, PK11195, midazolam, diazepam, clonazepam and flumazenil indicated that [3H]Ro5-4864 bound to specific receptors and binding was reversible. Scatchard analysis indicated a single class of binding site, with a dissociation constant (K(d)) of 12.8 ± 2.1 nM with maximum binding capacity of 2.6 ± 0.6 pmol/mg of protein for human lung and K(d) of 8.4 ± 1.5 nM and maximum binding capacity of 3.5 ± 0.3 pmol/mg of protein for guinea pig lung. Autoradiograms showed specific labeling over discrete structures in both human and guinea pig lung. Labeling was particularly dense over submucosal glands in intrapulmonary bronchi of human. Airways were also labeled, with epithelium having a higher grain density than smooth muscle. Labeling over smooth muscle was denser in small compared to large airways. Vascular smooth muscle was not labeled in either human and guinea pig. The alveolar walls were uniformly labeled in both species. The functional significance of pulmonary peripheral-type benzodiazepine receptors remains to be determined.en_US
dc.languageengen_US
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.orgen_US
dc.relation.ispartofJournal of Pharmacology and Experimental Therapeuticsen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBenzodiazepinones - Metabolismen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHumansen_US
dc.subject.meshIsoquinolines - Pharmacologyen_US
dc.subject.meshLung - Analysisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshReceptors, Gaba-A - Analysisen_US
dc.titlePeripheral type benzodiazepine receptors in human and guinea pig lung: Characterization and autoradiographic mappingen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2156066-
dc.identifier.scopuseid_2-s2.0-0025332587en_US
dc.identifier.volume252en_US
dc.identifier.issue2en_US
dc.identifier.spage880en_US
dc.identifier.epage885en_US
dc.identifier.isiWOS:A1990CT22200063-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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