File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Interferon-γ inhibits thyrotropin-induced thyroglobulin gene transcription in cultured human thyrocytes

TitleInterferon-γ inhibits thyrotropin-induced thyroglobulin gene transcription in cultured human thyrocytes
Authors
Issue Date1990
PublisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.org
Citation
Journal Of Clinical Endocrinology And Metabolism, 1990, v. 70 n. 6, p. 1512-1517 How to Cite?
AbstractIt has been shown that interferon-γ (IFNγ) can induce HLA class II antigen expression by thyroid epithelial cells and may play a role in the pathogenesis of autoimmune thyroid disease. We have examined the metabolic effect of recombinant human IFNγ (0.001-500 U/mL) on thyroglobulin (TG) synthesis and secretion in cultured human thyrocytes. Thyrocytes dispersed from human Graves' thyroid tissues were cultured in the presence of TSH with or without IFNγ. IFNγ alone had not effect on either basal TG secretion or de novo TG synthesis, as measured by immunoprecipitation. At 100 U/mL and above, IFNγ inhibited TSH-induced TG secretion into the medium. At 500 U/mL, IFNγ inhibited TSH- or dibutyryl cAMP-induced TG synthesis at the gene transcription level, as evidenced by the decrease in steady state TG mRNA. IFNγ had no effect on either basal or TSH-induced cAMP release by the thyrocytes, suggesting that the inhibitory effect occurs at a site distal to cAMP formation. These data demonstrate that IFNγ directly inhibits TSH-stimulated TG gene expression and TG secretion. This provides further evidence that IFNγ has a metabolic effect on thyroid hormone synthesis.
Persistent Identifierhttp://hdl.handle.net/10722/161837
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.940
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKung, AWCen_US
dc.contributor.authorLau, KSen_US
dc.date.accessioned2012-09-05T05:15:24Z-
dc.date.available2012-09-05T05:15:24Z-
dc.date.issued1990en_US
dc.identifier.citationJournal Of Clinical Endocrinology And Metabolism, 1990, v. 70 n. 6, p. 1512-1517en_US
dc.identifier.issn0021-972Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/161837-
dc.description.abstractIt has been shown that interferon-γ (IFNγ) can induce HLA class II antigen expression by thyroid epithelial cells and may play a role in the pathogenesis of autoimmune thyroid disease. We have examined the metabolic effect of recombinant human IFNγ (0.001-500 U/mL) on thyroglobulin (TG) synthesis and secretion in cultured human thyrocytes. Thyrocytes dispersed from human Graves' thyroid tissues were cultured in the presence of TSH with or without IFNγ. IFNγ alone had not effect on either basal TG secretion or de novo TG synthesis, as measured by immunoprecipitation. At 100 U/mL and above, IFNγ inhibited TSH-induced TG secretion into the medium. At 500 U/mL, IFNγ inhibited TSH- or dibutyryl cAMP-induced TG synthesis at the gene transcription level, as evidenced by the decrease in steady state TG mRNA. IFNγ had no effect on either basal or TSH-induced cAMP release by the thyrocytes, suggesting that the inhibitory effect occurs at a site distal to cAMP formation. These data demonstrate that IFNγ directly inhibits TSH-stimulated TG gene expression and TG secretion. This provides further evidence that IFNγ has a metabolic effect on thyroid hormone synthesis.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.orgen_US
dc.relation.ispartofJournal of Clinical Endocrinology and Metabolismen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCyclic Amp - Biosynthesisen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGraves Disease - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-Gamma - Pharmacologyen_US
dc.subject.meshPrecipitin Testsen_US
dc.subject.meshThyroglobulin - Genetics - Secretionen_US
dc.subject.meshThyroid Gland - Secretionen_US
dc.subject.meshThyrotropin - Physiologyen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleInterferon-γ inhibits thyrotropin-induced thyroglobulin gene transcription in cultured human thyrocytesen_US
dc.typeArticleen_US
dc.identifier.emailKung, AWC:awckung@hku.hken_US
dc.identifier.authorityKung, AWC=rp00368en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/jcem-70-6-1512-
dc.identifier.pmid2161422-
dc.identifier.scopuseid_2-s2.0-0025301268en_US
dc.identifier.volume70en_US
dc.identifier.issue6en_US
dc.identifier.spage1512en_US
dc.identifier.epage1517en_US
dc.identifier.isiWOS:A1990DG58800005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKung, AWC=7102322339en_US
dc.identifier.scopusauthoridLau, KS=35205833900en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats