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Article: Calcitonin gene-related peptide vasodilation of human pulmonary vessels

TitleCalcitonin gene-related peptide vasodilation of human pulmonary vessels
Authors
Issue Date1989
Citation
Journal Of Applied Physiology, 1989, v. 67 n. 3, p. 1265-1270 How to Cite?
AbstractHuman calcitonin gene-related peptide (CGRP) is localized to sensory neurons in pulmonary vessels and is a potent vasodilator. We have characterized the effects of CGRP in human pulmonary vessels and localized the receptors for this peptide by autoradiography. Fresh human lung tissue was obtained from eight patients undergoing surgery and small (200-400 μm ID) pulmonary arteries and veins were dissected free of surrounding connective and pulmonary tissue. Pairs of vessels were studied and in one of each pair the endothelium was left intact and from the other of each pair the endothelium was removed by gentle abrasion. For functional studies arteries (n = 9) and veins (n = 9) were suspended in an organ bath, precontracted with 1 μM prostaglandin F(2α). CGRP (10 pM to 10 μM) was added in a cumulative manner. CGRP caused a dose-dependent relaxation of endothelium intact human pulmonary arteries and veins with log EC50 values of -8.01 ± 0.35 and -8.70 ± 0.40, respectively (not significant). Removal of the endothelium did not diminish the vasodilator potency of CGRP in either vessel. For autoradiographic studies, cryostat sections of the small human pulmonary vessels with or without endothelium were used. 125I-CGRP densely labeled CGRP receptors on vascular smooth muscle and endothelial removal did not have any effect on grain density. We concluded that CGRP is a potent vasodilator of human pulmonary arteries and veins that is not dependent on an intact endothelium. These functional studies correlate with the distribution of CGRP receptors as localized by autoradiography.
Persistent Identifierhttp://hdl.handle.net/10722/161775
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMccormack, DGen_US
dc.contributor.authorMak, JCWen_US
dc.contributor.authorCoupe, MOen_US
dc.contributor.authorBarnes, PJen_US
dc.date.accessioned2012-09-05T05:14:51Z-
dc.date.available2012-09-05T05:14:51Z-
dc.date.issued1989en_US
dc.identifier.citationJournal Of Applied Physiology, 1989, v. 67 n. 3, p. 1265-1270en_US
dc.identifier.issn0161-7567en_US
dc.identifier.urihttp://hdl.handle.net/10722/161775-
dc.description.abstractHuman calcitonin gene-related peptide (CGRP) is localized to sensory neurons in pulmonary vessels and is a potent vasodilator. We have characterized the effects of CGRP in human pulmonary vessels and localized the receptors for this peptide by autoradiography. Fresh human lung tissue was obtained from eight patients undergoing surgery and small (200-400 μm ID) pulmonary arteries and veins were dissected free of surrounding connective and pulmonary tissue. Pairs of vessels were studied and in one of each pair the endothelium was left intact and from the other of each pair the endothelium was removed by gentle abrasion. For functional studies arteries (n = 9) and veins (n = 9) were suspended in an organ bath, precontracted with 1 μM prostaglandin F(2α). CGRP (10 pM to 10 μM) was added in a cumulative manner. CGRP caused a dose-dependent relaxation of endothelium intact human pulmonary arteries and veins with log EC50 values of -8.01 ± 0.35 and -8.70 ± 0.40, respectively (not significant). Removal of the endothelium did not diminish the vasodilator potency of CGRP in either vessel. For autoradiographic studies, cryostat sections of the small human pulmonary vessels with or without endothelium were used. 125I-CGRP densely labeled CGRP receptors on vascular smooth muscle and endothelial removal did not have any effect on grain density. We concluded that CGRP is a potent vasodilator of human pulmonary arteries and veins that is not dependent on an intact endothelium. These functional studies correlate with the distribution of CGRP receptors as localized by autoradiography.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshCalcitonin Gene-Related Peptide - Pharmacology - Physiologyen_US
dc.subject.meshEndothelium, Vascular - Drug Effects - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMuscle, Smooth, Vascular - Drug Effects - Physiologyen_US
dc.subject.meshPulmonary Artery - Anatomy & Histology - Drug Effects - Physiologyen_US
dc.subject.meshPulmonary Circulation - Drug Effectsen_US
dc.subject.meshPulmonary Veins - Anatomy & Histology - Drug Effects - Physiologyen_US
dc.subject.meshReceptors, Calcitoninen_US
dc.subject.meshReceptors, Cell Surface - Physiologyen_US
dc.subject.meshVasodilation - Drug Effectsen_US
dc.titleCalcitonin gene-related peptide vasodilation of human pulmonary vesselsen_US
dc.typeArticleen_US
dc.identifier.emailMak, JCW:judymak@hku.hken_US
dc.identifier.authorityMak, JCW=rp00352en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2551879-
dc.identifier.scopuseid_2-s2.0-0024330385en_US
dc.identifier.volume67en_US
dc.identifier.issue3en_US
dc.identifier.spage1265en_US
dc.identifier.epage1270en_US
dc.identifier.isiWOS:A1989AR32600053-
dc.identifier.scopusauthoridMcCormack, DG=7102878837en_US
dc.identifier.scopusauthoridMak, JCW=7103323094en_US
dc.identifier.scopusauthoridCoupe, MO=7003394131en_US
dc.identifier.scopusauthoridBarnes, PJ=36064679400en_US

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