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Article: In vitro study of expression of interleukin-2 receptors in T-lymphocytes from patients with IgA nephropathy

TitleIn vitro study of expression of interleukin-2 receptors in T-lymphocytes from patients with IgA nephropathy
Authors
Issue Date1988
PublisherDustri-Verlag Dr. Karl Feistle. The Journal's web site is located at http://www.clinnephrol.com
Citation
Clinical Nephrology, 1988, v. 30 n. 6, p. 330-334 How to Cite?
AbstractThe present study was undertaken to examine the expression of interleukin-2 receptor (IL-2R)/CD25 antigen in cultured T-lymphocyte subsets in IgA nephropathy. Twenty-four IgA nephritic patients, 12 patients with chronic glomerulonephritis (non-IgA nephropathy), and 17 healthy controls were studied in an infection-free interval. T-cell subsets in peripheral blood mononuclear cells (PBMC) and activated T-lymphcoyte subsets expressing IL-2R were determined by double immunofluorescence staining with fluorochromes conjugated to monoclonal antibodies against T-helper/inducer (CD4+) cell, T-suppressor/cytotoxic (CD8+) cell, B (CD20+) lymphocytes, and IL-2R. The percentages of CD4+ and CD8+ lymphocytes, CD4/CD8 ratio, and total activated lymphocytes (with IL-2R/CD25 antigen) did not differ between the IgA nephritic patients, patients with chronic glomerulonephritis, and healthy controls in freshly isolated, unstimulated lymphocytes or PBMC cultured with pokeweed mitogen. Following pokeweed mitogen stimulation for 5 days, 17.3 ± 10.3%, 16.6 ± 8.4%, and 16.7 ± 9.4% of PBMC from IgA nephritic patients, patients with chronic glomerulonephritis, and controls respectively expressed IL-2R (p > 0.05). However, the individual T-cell subsets bearing IL-2R were distinctly different between the IgA nephritic patients and patients with chronic glomerulonephritis or healthy controls. IgA nephritic patients had increased activated CD4+ lymphocytes (with IL-2R) (p < 0.025) and reduced activated CD8+ lymphocytes (p < 0.025). Our study suggests a defective immunoregulation in IgA nephropathy with enhanced T-helper/inducer and reduced T-suppressor/cytotoxic activity when stimulated with mitogen and probably, during clinical exacerbation.
Persistent Identifierhttp://hdl.handle.net/10722/161772
ISSN
2015 Impact Factor: 1.065
2015 SCImago Journal Rankings: 0.498
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorMacMoune Lai, Fen_HK
dc.date.accessioned2012-09-05T05:14:51Z-
dc.date.available2012-09-05T05:14:51Z-
dc.date.issued1988en_HK
dc.identifier.citationClinical Nephrology, 1988, v. 30 n. 6, p. 330-334en_HK
dc.identifier.issn0301-0430en_HK
dc.identifier.urihttp://hdl.handle.net/10722/161772-
dc.description.abstractThe present study was undertaken to examine the expression of interleukin-2 receptor (IL-2R)/CD25 antigen in cultured T-lymphocyte subsets in IgA nephropathy. Twenty-four IgA nephritic patients, 12 patients with chronic glomerulonephritis (non-IgA nephropathy), and 17 healthy controls were studied in an infection-free interval. T-cell subsets in peripheral blood mononuclear cells (PBMC) and activated T-lymphcoyte subsets expressing IL-2R were determined by double immunofluorescence staining with fluorochromes conjugated to monoclonal antibodies against T-helper/inducer (CD4+) cell, T-suppressor/cytotoxic (CD8+) cell, B (CD20+) lymphocytes, and IL-2R. The percentages of CD4+ and CD8+ lymphocytes, CD4/CD8 ratio, and total activated lymphocytes (with IL-2R/CD25 antigen) did not differ between the IgA nephritic patients, patients with chronic glomerulonephritis, and healthy controls in freshly isolated, unstimulated lymphocytes or PBMC cultured with pokeweed mitogen. Following pokeweed mitogen stimulation for 5 days, 17.3 ± 10.3%, 16.6 ± 8.4%, and 16.7 ± 9.4% of PBMC from IgA nephritic patients, patients with chronic glomerulonephritis, and controls respectively expressed IL-2R (p > 0.05). However, the individual T-cell subsets bearing IL-2R were distinctly different between the IgA nephritic patients and patients with chronic glomerulonephritis or healthy controls. IgA nephritic patients had increased activated CD4+ lymphocytes (with IL-2R) (p < 0.025) and reduced activated CD8+ lymphocytes (p < 0.025). Our study suggests a defective immunoregulation in IgA nephropathy with enhanced T-helper/inducer and reduced T-suppressor/cytotoxic activity when stimulated with mitogen and probably, during clinical exacerbation.en_HK
dc.languageengen_US
dc.publisherDustri-Verlag Dr. Karl Feistle. The Journal's web site is located at http://www.clinnephrol.comen_HK
dc.relation.ispartofClinical Nephrologyen_HK
dc.subject.meshAdulten_US
dc.subject.meshFemaleen_US
dc.subject.meshFluoroimmunoassayen_US
dc.subject.meshGlomerulonephritis - Immunologyen_US
dc.subject.meshGlomerulonephritis, Iga - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshPokeweed Mitogens - Pharmacologyen_US
dc.subject.meshReceptors, Interleukin-2 - Metabolismen_US
dc.subject.meshT-Lymphocytes - Classification - Immunologyen_US
dc.subject.meshT-Lymphocytes, Helper-Inducer - Immunologyen_US
dc.subject.meshT-Lymphocytes, Regulatory - Immunologyen_US
dc.titleIn vitro study of expression of interleukin-2 receptors in T-lymphocytes from patients with IgA nephropathyen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2977310-
dc.identifier.scopuseid_2-s2.0-0024267499en_HK
dc.identifier.volume30en_HK
dc.identifier.issue6en_HK
dc.identifier.spage330en_HK
dc.identifier.epage334en_HK
dc.identifier.isiWOS:A1988R392600006-
dc.publisher.placeMünchen-Deisenhofen, Germanyen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridMacMoune Lai, F=6701570301en_HK

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