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Article: Effect of Graves' IgG on gene transcription in human thyroid cell cultures. Thyroglobulin gene activation

TitleEffect of Graves' IgG on gene transcription in human thyroid cell cultures. Thyroglobulin gene activation
Authors
Issue Date1988
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 1988, v. 232 n. 1, p. 12-16 How to Cite?
AbstractIn Graves' disease (GD) the presence of antibodies to the thyroid stimulating hormone (TSH) receptor leads to stimulation of the thyroid gland. The thyroid stimulating activity of Graves' IgG is normally ascertained by bioassays measuring cAMP production. We have investigated the effect of Graves' IgG on the quantitative activation of thyroglobulin (TG) gene in cultured human thyroid cells by RNA hybridisation. TG mRNA expression was activated by TSH and Graves' IgG. Nuclear transcription assays showed that the increase in cytoplasmic mRNA levels was due to increased transcription of TG specific mRNA in nuclei of thyroid cells. Whilst TSH led to a dose dependent increase in TG mRNA levels, Graves' IgG led to a variable activation of TG gene. A significant correlation between the increased TG mRNA transcription and cAMP production was observed with Graves' IgG. Thus the activation of the TG gene by Graves' IgG occurs in parallel with elevation of cAMP.
Persistent Identifierhttp://hdl.handle.net/10722/161766
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKung, AWCen_US
dc.contributor.authorCollison, Ken_US
dc.contributor.authorBanga, JPen_US
dc.contributor.authorMcgregor, AMen_US
dc.date.accessioned2012-09-05T05:14:46Z-
dc.date.available2012-09-05T05:14:46Z-
dc.date.issued1988en_US
dc.identifier.citationFebs Letters, 1988, v. 232 n. 1, p. 12-16en_US
dc.identifier.issn0014-5793en_US
dc.identifier.urihttp://hdl.handle.net/10722/161766-
dc.description.abstractIn Graves' disease (GD) the presence of antibodies to the thyroid stimulating hormone (TSH) receptor leads to stimulation of the thyroid gland. The thyroid stimulating activity of Graves' IgG is normally ascertained by bioassays measuring cAMP production. We have investigated the effect of Graves' IgG on the quantitative activation of thyroglobulin (TG) gene in cultured human thyroid cells by RNA hybridisation. TG mRNA expression was activated by TSH and Graves' IgG. Nuclear transcription assays showed that the increase in cytoplasmic mRNA levels was due to increased transcription of TG specific mRNA in nuclei of thyroid cells. Whilst TSH led to a dose dependent increase in TG mRNA levels, Graves' IgG led to a variable activation of TG gene. A significant correlation between the increased TG mRNA transcription and cAMP production was observed with Graves' IgG. Thus the activation of the TG gene by Graves' IgG occurs in parallel with elevation of cAMP.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_US
dc.relation.ispartofFEBS Lettersen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclic Amp - Biosynthesisen_US
dc.subject.meshForskolin - Pharmacologyen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshGraves Disease - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G - Physiologyen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshTetradecanoylphorbol Acetate - Pharmacologyen_US
dc.subject.meshThyroglobulin - Geneticsen_US
dc.subject.meshThyroid Gland - Metabolismen_US
dc.subject.meshTranscription, Genetic - Drug Effectsen_US
dc.subject.meshTranscriptional Activationen_US
dc.titleEffect of Graves' IgG on gene transcription in human thyroid cell cultures. Thyroglobulin gene activationen_US
dc.typeArticleen_US
dc.identifier.emailKung, AWC:awckung@hku.hken_US
dc.identifier.authorityKung, AWC=rp00368en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0014-5793(88)80377-6en_US
dc.identifier.pmid2835260-
dc.identifier.scopuseid_2-s2.0-0023938710en_US
dc.identifier.volume232en_US
dc.identifier.issue1en_US
dc.identifier.spage12en_US
dc.identifier.epage16en_US
dc.identifier.isiWOS:A1988N425500003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridKung, AWC=7102322339en_US
dc.identifier.scopusauthoridCollison, K=36902959400en_US
dc.identifier.scopusauthoridBanga, JP=7102475139en_US
dc.identifier.scopusauthoridMcGregor, AM=7102812103en_US

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