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Article: Cell-free synthesis of factor VIII related protein

TitleCell-free synthesis of factor VIII related protein
Authors
Issue Date1983
PublisherSchattauer GmbH. The Journal's web site is located at http://www.thrombosis-online.com
Citation
Thrombosis And Haemostasis, 1983, v. 50 n. 4, p. 835-837 How to Cite?
AbstractPolyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunoprecipitation, the translation product was found to contain 1.58% VIII related protein (VIII R), which has a molecular weight of 0.26 x 106, similar to the subunit of VIII R present in normal plasma. This indicated that VIII R messenger RNA is responsible for the synthesis of subunits only and intracellular post-translational events are required for the assembly of tetramer and multimers.
Persistent Identifierhttp://hdl.handle.net/10722/161673
ISSN
2015 Impact Factor: 5.255
2015 SCImago Journal Rankings: 2.089
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Ven_US
dc.contributor.authorChan, TKen_US
dc.date.accessioned2012-09-05T05:13:45Z-
dc.date.available2012-09-05T05:13:45Z-
dc.date.issued1983en_US
dc.identifier.citationThrombosis And Haemostasis, 1983, v. 50 n. 4, p. 835-837en_US
dc.identifier.issn0340-6245en_US
dc.identifier.urihttp://hdl.handle.net/10722/161673-
dc.description.abstractPolyadenylated ribonucleic acid (RNA) was isolated from confluent human endothelial cells in culture and translated in a cell-free rabbit reticulocyte lysate protein-synthesizing system. Using specific immunoprecipitation, the translation product was found to contain 1.58% VIII related protein (VIII R), which has a molecular weight of 0.26 x 106, similar to the subunit of VIII R present in normal plasma. This indicated that VIII R messenger RNA is responsible for the synthesis of subunits only and intracellular post-translational events are required for the assembly of tetramer and multimers.en_US
dc.languageengen_US
dc.publisherSchattauer GmbH. The Journal's web site is located at http://www.thrombosis-online.comen_US
dc.relation.ispartofThrombosis and Haemostasisen_US
dc.subject.meshAntigens - Biosynthesis - Geneticsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshCell-Free Systemen_US
dc.subject.meshElectrophoresis, Agar Gelen_US
dc.subject.meshFactor Viii - Biosynthesis - Genetics - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshPoly A - Isolation & Purificationen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRna - Isolation & Purificationen_US
dc.subject.meshRna, Messenger - Isolation & Purificationen_US
dc.subject.meshVon Willebrand Factoren_US
dc.titleCell-free synthesis of factor VIII related proteinen_US
dc.typeArticleen_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6198745-
dc.identifier.scopuseid_2-s2.0-0021072226en_US
dc.identifier.volume50en_US
dc.identifier.issue4en_US
dc.identifier.spage835en_US
dc.identifier.epage837en_US
dc.identifier.isiWOS:A1983RY33300017-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridChan, V=7202654865en_US
dc.identifier.scopusauthoridChan, TK=7402687762en_US

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