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Article: Divergent effects of prolactin on estrogen and progesterone production by granulosa cells of rat Graafian follicles.

TitleDivergent effects of prolactin on estrogen and progesterone production by granulosa cells of rat Graafian follicles.
Authors
Issue Date1982
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 1982, v. 110 n. 4, p. 1085-1093 How to Cite?
AbstractThe effect of PRL on ovarian steroidogenesis was studied in cultured granulosa cells isolated from follicles of mature cycling rats on the morning of proestrus. Ovine PRL 10-1000 ng/ml) inhibited estradiol production but stimulated progesterone biosynthesis in a dose-dependent manner. The effect of PRL was most prominent after 4 days of culture: 1000 ng/ml PRL suppressed estradiol production by 80% but increased progesterone synthesis by 290%, whereas the lower dose of 10 ng/ml inhibited estrogen secretion by 20% without altering progesterone synthesis. The divergent effect of PRL was not shown to be species specific, since ovine, rat and human PRL had similar effects. Using increasing concentrations of androstenedione (the aromatase substrate), estrogen secretion remained suppressed and progesterone production was stimulated by PRL. FSH stimulated both estrogen and progesterone production. The FSH-induced increased in estrogen production was inhibited by concomitant treatment with PRL. In contrast, PRL and FSH had an additive action in stimulating progesterone production. Although LH alone had no effect on steroidogenesis, concomitant treatment with LH and PRL resulted in a stimulation of progesterone production that was additive. This study demonstrates that PRL acts directly on granulosa cells of Graafian follicles of adult cycling rats to stimulate the secretion of progesterone and to suppress estradiol production.
Persistent Identifierhttp://hdl.handle.net/10722/161665
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Cen_US
dc.contributor.authorChan, Ven_US
dc.date.accessioned2012-09-05T05:13:41Z-
dc.date.available2012-09-05T05:13:41Z-
dc.date.issued1982en_US
dc.identifier.citationEndocrinology, 1982, v. 110 n. 4, p. 1085-1093en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/161665-
dc.description.abstractThe effect of PRL on ovarian steroidogenesis was studied in cultured granulosa cells isolated from follicles of mature cycling rats on the morning of proestrus. Ovine PRL 10-1000 ng/ml) inhibited estradiol production but stimulated progesterone biosynthesis in a dose-dependent manner. The effect of PRL was most prominent after 4 days of culture: 1000 ng/ml PRL suppressed estradiol production by 80% but increased progesterone synthesis by 290%, whereas the lower dose of 10 ng/ml inhibited estrogen secretion by 20% without altering progesterone synthesis. The divergent effect of PRL was not shown to be species specific, since ovine, rat and human PRL had similar effects. Using increasing concentrations of androstenedione (the aromatase substrate), estrogen secretion remained suppressed and progesterone production was stimulated by PRL. FSH stimulated both estrogen and progesterone production. The FSH-induced increased in estrogen production was inhibited by concomitant treatment with PRL. In contrast, PRL and FSH had an additive action in stimulating progesterone production. Although LH alone had no effect on steroidogenesis, concomitant treatment with LH and PRL resulted in a stimulation of progesterone production that was additive. This study demonstrates that PRL acts directly on granulosa cells of Graafian follicles of adult cycling rats to stimulate the secretion of progesterone and to suppress estradiol production.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.subject.meshAndrostenedione - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDrug Interactionsen_US
dc.subject.meshEstradiol - Biosynthesisen_US
dc.subject.meshFemaleen_US
dc.subject.meshFollicle Stimulating Hormone - Pharmacologyen_US
dc.subject.meshGranulosa Cells - Drug Effects - Metabolismen_US
dc.subject.meshLuteinizing Hormone - Pharmacologyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshProestrusen_US
dc.subject.meshProgesterone - Biosynthesisen_US
dc.subject.meshProlactin - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.titleDivergent effects of prolactin on estrogen and progesterone production by granulosa cells of rat Graafian follicles.en_US
dc.typeArticleen_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/endo-110-4-1085-
dc.identifier.pmid6800769-
dc.identifier.scopuseid_2-s2.0-0020120213en_US
dc.identifier.volume110en_US
dc.identifier.issue4en_US
dc.identifier.spage1085en_US
dc.identifier.epage1093en_US
dc.identifier.isiWOS:A1982NF98800002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWang, C=7501631357en_US
dc.identifier.scopusauthoridChan, V=7202654865en_US

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