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Article: Heparin-antithrombin III binding. In vitro and in vivo studies

TitleHeparin-antithrombin III binding. In vitro and in vivo studies
Authors
Issue Date1979
Citation
Haemostasis, 1979, v. 8 n. 6, p. 373-389 How to Cite?
AbstractHeparin antithrombin III binding was studied by crossed immunoelectrophoresis. In plasma and purified antithrombin III standard, multicomponent patterns were obtained with low concentrations of mucosal heparin. There is evidence that antithrombin III may bind more than one heparin molecule. At high heparin concentration (>16 U/ml), single symmetrical peaks were obtained. Serum samples showed two antithrombin III peaks due to a decreased heparin binding of the slower peak (2.1-3.9 times), which was probably antithrombin III-activated procoagulant complexes. Heparin analogue (A 73025) also bound antithrombin III in vitro but the mobility of the peak was slower than with mucosal heparin and only a single peak was obtained in serum samples. Radioimmunoassay showed a decreased binding of antithrombin III antibody to heparin-antithrombin III complex. Venous occlusion to the forearm resulted in a slow second peak in the plasma. Heparin therapy gave rise to a double peak in the plasma antithrombin III profile and with continuous infusion, quantitative decreases were noted in all subjects studied, two of whom rethrombosed at the end of 7 days therapy.
Persistent Identifierhttp://hdl.handle.net/10722/161644
ISSN
2003 Impact Factor: 1.0
2004 SCImago Journal Rankings: 0.354
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Ven_US
dc.contributor.authorChan, TKen_US
dc.date.accessioned2012-09-05T05:13:27Z-
dc.date.available2012-09-05T05:13:27Z-
dc.date.issued1979en_US
dc.identifier.citationHaemostasis, 1979, v. 8 n. 6, p. 373-389en_US
dc.identifier.issn0301-0147en_US
dc.identifier.urihttp://hdl.handle.net/10722/161644-
dc.description.abstractHeparin antithrombin III binding was studied by crossed immunoelectrophoresis. In plasma and purified antithrombin III standard, multicomponent patterns were obtained with low concentrations of mucosal heparin. There is evidence that antithrombin III may bind more than one heparin molecule. At high heparin concentration (>16 U/ml), single symmetrical peaks were obtained. Serum samples showed two antithrombin III peaks due to a decreased heparin binding of the slower peak (2.1-3.9 times), which was probably antithrombin III-activated procoagulant complexes. Heparin analogue (A 73025) also bound antithrombin III in vitro but the mobility of the peak was slower than with mucosal heparin and only a single peak was obtained in serum samples. Radioimmunoassay showed a decreased binding of antithrombin III antibody to heparin-antithrombin III complex. Venous occlusion to the forearm resulted in a slow second peak in the plasma. Heparin therapy gave rise to a double peak in the plasma antithrombin III profile and with continuous infusion, quantitative decreases were noted in all subjects studied, two of whom rethrombosed at the end of 7 days therapy.en_US
dc.languageengen_US
dc.relation.ispartofHaemostasisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntithrombin Iii - Immunology - Pharmacologyen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshBinding Sites, Antibodyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshHeparin - Analogs & Derivatives - Pharmacology - Therapeutic Useen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoelectrophoresis, Two-Dimensionalen_US
dc.subject.meshRabbitsen_US
dc.subject.meshRadioimmunoassayen_US
dc.subject.meshThrombophlebitis - Drug Therapy - Immunologyen_US
dc.subject.meshTime Factorsen_US
dc.titleHeparin-antithrombin III binding. In vitro and in vivo studiesen_US
dc.typeArticleen_US
dc.identifier.emailChan, V:vnychana@hkucc.hku.hken_US
dc.identifier.authorityChan, V=rp00320en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid511016-
dc.identifier.scopuseid_2-s2.0-0018627176en_US
dc.identifier.volume8en_US
dc.identifier.issue6en_US
dc.identifier.spage373en_US
dc.identifier.epage389en_US
dc.identifier.isiWOS:A1979HV38300002-
dc.identifier.scopusauthoridChan, V=7202654865en_US
dc.identifier.scopusauthoridChan, TK=7402687762en_US

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