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Article: Pharmacological induction of leukotriene B4-12-hydroxydehydrogenase suppresses the oncogenic transformation of human hepatoma HepG2 cells

TitlePharmacological induction of leukotriene B4-12-hydroxydehydrogenase suppresses the oncogenic transformation of human hepatoma HepG2 cells
Authors
KeywordsBioactivity-guided fractionation
Cell cycle arrest
Chemoprevention
Gallic acid
Leukotriene B4
Leukotriene B4-12-hydroxydehydrogenase
Oncogenic transformation
Radix astragali
Radix paeoniae rubra
Issue Date2011
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 2011, v. 39 n. 3, p. 735-745 How to Cite?
AbstractLeukotriene B4-12-hydroxydehydrogenase (LTB4DH) is characterized as a chemopreventive and tumor suppressor gene. The aim of this study was to investigate the pharmacological induction of LTB4DH and potential anticancer activity. Using HepG2 cells as a cellular detector, we successfully isolated the active compounds from the herbs Radix Astragali and Radix Paeoniae Rubra through a bioactivity-guided fractionation procedure. Using various analytical techniques including electronic spray ionization-mass spectrometry (ESIMS) and nuclear magnetic resonance (NMR), gallic acid (GA) was identified as the active compound from Radix Paeoniae Rubra whereas the active compound from Radix Astragali, designated as RA-C, was also purified to the extent that it is now suitable for further identification. We found that the active compounds from these two different herbs synergistically induced LTB4DH expression in a dose- and time-dependent manner. A key finding was that commercial GA in combination with purified RA-C attenuated the focus formation and anchorage-independent growth, two indexes of in vitro oncogenic transformation, of HepG2 cells via the induction of LTB4DH expression. Moreover, the combination of GA and purified RA-C significantly induced G2/M cell cycle arrest in HepG2 cells. Our results demonstrated for the first time that GA and purified RA-C suppress the in vitro oncogenic transformation of HepG2 cells via the induction of LTB4DH expression. Importantly, pharmacological induction of LTB4DH represents a potential alternative strategy for the therapy of hepatocellular carcinoma.
Persistent Identifierhttp://hdl.handle.net/10722/161596
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWei, Len_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorLe, XCen_HK
dc.contributor.authorHan, Yen_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorLau, ASYen_HK
dc.contributor.authorRong, Jen_HK
dc.date.accessioned2012-09-03T07:20:40Z-
dc.date.available2012-09-03T07:20:40Z-
dc.date.issued2011en_HK
dc.identifier.citationInternational Journal Of Oncology, 2011, v. 39 n. 3, p. 735-745en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/161596-
dc.description.abstractLeukotriene B4-12-hydroxydehydrogenase (LTB4DH) is characterized as a chemopreventive and tumor suppressor gene. The aim of this study was to investigate the pharmacological induction of LTB4DH and potential anticancer activity. Using HepG2 cells as a cellular detector, we successfully isolated the active compounds from the herbs Radix Astragali and Radix Paeoniae Rubra through a bioactivity-guided fractionation procedure. Using various analytical techniques including electronic spray ionization-mass spectrometry (ESIMS) and nuclear magnetic resonance (NMR), gallic acid (GA) was identified as the active compound from Radix Paeoniae Rubra whereas the active compound from Radix Astragali, designated as RA-C, was also purified to the extent that it is now suitable for further identification. We found that the active compounds from these two different herbs synergistically induced LTB4DH expression in a dose- and time-dependent manner. A key finding was that commercial GA in combination with purified RA-C attenuated the focus formation and anchorage-independent growth, two indexes of in vitro oncogenic transformation, of HepG2 cells via the induction of LTB4DH expression. Moreover, the combination of GA and purified RA-C significantly induced G2/M cell cycle arrest in HepG2 cells. Our results demonstrated for the first time that GA and purified RA-C suppress the in vitro oncogenic transformation of HepG2 cells via the induction of LTB4DH expression. Importantly, pharmacological induction of LTB4DH represents a potential alternative strategy for the therapy of hepatocellular carcinoma.en_HK
dc.languageeng-
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational Journal of Oncologyen_HK
dc.subjectBioactivity-guided fractionationen_HK
dc.subjectCell cycle arresten_HK
dc.subjectChemopreventionen_HK
dc.subjectGallic aciden_HK
dc.subjectLeukotriene B4en_HK
dc.subjectLeukotriene B4-12-hydroxydehydrogenaseen_HK
dc.subjectOncogenic transformationen_HK
dc.subjectRadix astragalien_HK
dc.subjectRadix paeoniae rubraen_HK
dc.subject.meshAlcohol Oxidoreductases - biosynthesis-
dc.subject.meshCarcinoma, Hepatocellular - enzymology - genetics - pathology - prevention and control-
dc.subject.meshCell Transformation, Neoplastic - drug effects - genetics - metabolism-
dc.subject.meshEnzyme Induction - drug effects-
dc.subject.meshHep G2 Cells - metabolism-
dc.titlePharmacological induction of leukotriene B4-12-hydroxydehydrogenase suppresses the oncogenic transformation of human hepatoma HepG2 cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.emailLau, ASY: asylau@hku.hken_HK
dc.identifier.emailRong, J: jrong@hku.hken_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.identifier.authorityLau, ASY=rp00474en_HK
dc.identifier.authorityRong, J=rp00515en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3892/ijo.2011.1082en_HK
dc.identifier.pmid21687936-
dc.identifier.scopuseid_2-s2.0-79959949258en_HK
dc.identifier.hkuros187397-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79959949258&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue3en_HK
dc.identifier.spage735en_HK
dc.identifier.epage745en_HK
dc.identifier.isiWOS:000293492900024-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridWei, L=43861929300en_HK
dc.identifier.scopusauthoridLiu, J=43861639000en_HK
dc.identifier.scopusauthoridLe, XC=7007054458en_HK
dc.identifier.scopusauthoridHan, Y=8527680500en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridLau, ASY=7202626202en_HK
dc.identifier.scopusauthoridRong, J=7005980047en_HK

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