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Postgraduate Thesis: Characterization of hMSCs transmigrated through collagen barrier
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TitleCharacterization of hMSCs transmigrated through collagen barrier
 
AuthorsWong, Yin-kwai.
王現葵.
 
Issue Date2011
 
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
AbstractStem cell therapy is a promising approach for tissue regeneration but there exists a problem of low engraftment rate to the injury site. Our laboratory has shown that hMSCs that were capable to penetrate through collagen barrier have higher migration capacity and engraftment efficiency than those remained inside the collagen matrix and those in traditional 2D culture. These cells capable of penetrating through collagen barrier might be hopeful candidate for improving engraftment efficacy. Major processes of engraftment, such as transmigration through basement membrane and invasion to the site of injury, involve cell-extracellular matrix-interacting proteins. As matrix metalloproteinases (MMP) and integrins are the key players in these processes, MMP and integrin profiles of the hMSCs were studied In this study, we demonstrated that hMSCs that were capable of penetrating through the collagen barrier have distinctive MMP profile to traditional 2D culture. These cells secrete significantly higher amount of MMP-1 than 2D culture, but the amount of MMP-2 secreted is comparable to traditional 2D culture. On the other hand, MMP-9 and MMP-13 were below detection limit by ELISA in both groups. Moreover, we have investigated the subcellular localization of MMPs and integrins. The cells were seeded on dishes with or without ECM coatings. It was demonstrated that hMSCs capable of penetrating through collagen barrier exhibit higher amount of subcellular MT1-MMP and integrin 6271 on ECM coated dish. Moreover, these cells exhibit a prominent feature of perinuclear localization of MT1-MMP., whereas the level of subcellular MMP-2, integrin 65 and 6v73 is comparable to that in 2D culture. We have also investigated the stem properties of hMSCs penetrated through collagen barrier. These properties include proliferation capacity, self-renewal capacity and differentiation potential towards chondrogenic, osteogenic and adipogenic lineages. It has been demonstrated that these properties are not compromised for superior migratory activities.
 
AdvisorsChan, BP
 
DegreeMaster of Philosophy
 
SubjectStem cells.
Mesenchyme.
 
Dept/ProgramMechanical Engineering
 
DC FieldValue
dc.contributor.advisorChan, BP
 
dc.contributor.authorWong, Yin-kwai.
 
dc.contributor.author王現葵.
 
dc.date.hkucongregation2012
 
dc.date.issued2011
 
dc.description.abstractStem cell therapy is a promising approach for tissue regeneration but there exists a problem of low engraftment rate to the injury site. Our laboratory has shown that hMSCs that were capable to penetrate through collagen barrier have higher migration capacity and engraftment efficiency than those remained inside the collagen matrix and those in traditional 2D culture. These cells capable of penetrating through collagen barrier might be hopeful candidate for improving engraftment efficacy. Major processes of engraftment, such as transmigration through basement membrane and invasion to the site of injury, involve cell-extracellular matrix-interacting proteins. As matrix metalloproteinases (MMP) and integrins are the key players in these processes, MMP and integrin profiles of the hMSCs were studied In this study, we demonstrated that hMSCs that were capable of penetrating through the collagen barrier have distinctive MMP profile to traditional 2D culture. These cells secrete significantly higher amount of MMP-1 than 2D culture, but the amount of MMP-2 secreted is comparable to traditional 2D culture. On the other hand, MMP-9 and MMP-13 were below detection limit by ELISA in both groups. Moreover, we have investigated the subcellular localization of MMPs and integrins. The cells were seeded on dishes with or without ECM coatings. It was demonstrated that hMSCs capable of penetrating through collagen barrier exhibit higher amount of subcellular MT1-MMP and integrin 6271 on ECM coated dish. Moreover, these cells exhibit a prominent feature of perinuclear localization of MT1-MMP., whereas the level of subcellular MMP-2, integrin 65 and 6v73 is comparable to that in 2D culture. We have also investigated the stem properties of hMSCs penetrated through collagen barrier. These properties include proliferation capacity, self-renewal capacity and differentiation potential towards chondrogenic, osteogenic and adipogenic lineages. It has been demonstrated that these properties are not compromised for superior migratory activities.
 
dc.description.naturepublished_or_final_version
 
dc.description.thesisdisciplineMechanical Engineering
 
dc.description.thesislevelmaster's
 
dc.description.thesisnameMaster of Philosophy
 
dc.identifier.hkulb4786972
 
dc.languageeng
 
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)
 
dc.relation.ispartofHKU Theses Online (HKUTO)
 
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.source.urihttp://hub.hku.hk/bib/B47869720
 
dc.subject.lcshStem cells.
 
dc.subject.lcshMesenchyme.
 
dc.titleCharacterization of hMSCs transmigrated through collagen barrier
 
dc.typePG_Thesis
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.advisor>Chan, BP</contributor.advisor>
<contributor.author>Wong, Yin-kwai.</contributor.author>
<contributor.author>&#29579;&#29694;&#33909;.</contributor.author>
<date.issued>2011</date.issued>
<description.abstract>&#65279;Stem cell therapy is a promising approach for tissue regeneration but there exists a

problem of low engraftment rate to the injury site. Our laboratory has shown that

hMSCs that were capable to penetrate through collagen barrier have higher

migration capacity and engraftment efficiency than those remained inside the

collagen matrix and those in traditional 2D culture. These cells capable of

penetrating through collagen barrier might be hopeful candidate for improving

engraftment efficacy. Major processes of engraftment, such as transmigration

through basement membrane and invasion to the site of injury, involve

cell-extracellular matrix-interacting proteins. As matrix metalloproteinases (MMP)

and integrins are the key players in these processes, MMP and integrin profiles of

the hMSCs were studied

In this study, we demonstrated that hMSCs that were capable of penetrating

through the collagen barrier have distinctive MMP profile to traditional 2D culture.

These cells secrete significantly higher amount of MMP-1 than 2D culture, but the

amount of MMP-2 secreted is comparable to traditional 2D culture. On the other

hand, MMP-9 and MMP-13 were below detection limit by ELISA in both groups.

Moreover, we have investigated the subcellular localization of MMPs and

integrins. The cells were seeded on dishes with or without ECM coatings. It was

demonstrated that hMSCs capable of penetrating through collagen barrier exhibit

higher amount of subcellular MT1-MMP and integrin 6271 on ECM coated dish.

Moreover, these cells exhibit a prominent feature of perinuclear localization of

MT1-MMP., whereas the level of subcellular MMP-2, integrin 65 and 6v73 is

comparable to that in 2D culture.

We have also investigated the stem properties of hMSCs penetrated through

collagen barrier. These properties include proliferation capacity, self-renewal

capacity and differentiation potential towards chondrogenic, osteogenic and

adipogenic lineages. It has been demonstrated that these properties are not

compromised for superior migratory activities.</description.abstract>
<language>eng</language>
<publisher>The University of Hong Kong (Pokfulam, Hong Kong)</publisher>
<relation.ispartof>HKU Theses Online (HKUTO)</relation.ispartof>
<rights>The author retains all proprietary rights, (such as patent rights) and the right to use in future works.</rights>
<rights>Creative Commons: Attribution 3.0 Hong Kong License</rights>
<source.uri>http://hub.hku.hk/bib/B47869720</source.uri>
<subject.lcsh>Stem cells.</subject.lcsh>
<subject.lcsh>Mesenchyme.</subject.lcsh>
<title>Characterization of hMSCs transmigrated through collagen barrier</title>
<type>PG_Thesis</type>
<identifier.hkul>b4786972</identifier.hkul>
<description.thesisname>Master of Philosophy</description.thesisname>
<description.thesislevel>master&apos;s</description.thesislevel>
<description.thesisdiscipline>Mechanical Engineering</description.thesisdiscipline>
<description.nature>published_or_final_version</description.nature>
<date.hkucongregation>2012</date.hkucongregation>
<bitstream.url>http://hub.hku.hk/bitstream/10722/161540/1/FullText.pdf</bitstream.url>
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