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postgraduate thesis: Characterization of hMSCs transmigrated through collagen barrier

TitleCharacterization of hMSCs transmigrated through collagen barrier
Authors
Advisors
Advisor(s):Chan, BP
Issue Date2011
PublisherThe University of Hong Kong (Pokfulam, Hong Kong)
Abstract
Stem cell therapy is a promising approach for tissue regeneration but there exists a problem of low engraftment rate to the injury site. Our laboratory has shown that hMSCs that were capable to penetrate through collagen barrier have higher migration capacity and engraftment efficiency than those remained inside the collagen matrix and those in traditional 2D culture. These cells capable of penetrating through collagen barrier might be hopeful candidate for improving engraftment efficacy. Major processes of engraftment, such as transmigration through basement membrane and invasion to the site of injury, involve cell-extracellular matrix-interacting proteins. As matrix metalloproteinases (MMP) and integrins are the key players in these processes, MMP and integrin profiles of the hMSCs were studied In this study, we demonstrated that hMSCs that were capable of penetrating through the collagen barrier have distinctive MMP profile to traditional 2D culture. These cells secrete significantly higher amount of MMP-1 than 2D culture, but the amount of MMP-2 secreted is comparable to traditional 2D culture. On the other hand, MMP-9 and MMP-13 were below detection limit by ELISA in both groups. Moreover, we have investigated the subcellular localization of MMPs and integrins. The cells were seeded on dishes with or without ECM coatings. It was demonstrated that hMSCs capable of penetrating through collagen barrier exhibit higher amount of subcellular MT1-MMP and integrin 6271 on ECM coated dish. Moreover, these cells exhibit a prominent feature of perinuclear localization of MT1-MMP., whereas the level of subcellular MMP-2, integrin 65 and 6v73 is comparable to that in 2D culture. We have also investigated the stem properties of hMSCs penetrated through collagen barrier. These properties include proliferation capacity, self-renewal capacity and differentiation potential towards chondrogenic, osteogenic and adipogenic lineages. It has been demonstrated that these properties are not compromised for superior migratory activities.
DegreeMaster of Philosophy
SubjectStem cells.
Mesenchyme.
Dept/ProgramMechanical Engineering

 

DC FieldValueLanguage
dc.contributor.advisorChan, BP-
dc.contributor.authorWong, Yin-kwai.-
dc.contributor.author王現葵.-
dc.date.issued2011-
dc.description.abstractStem cell therapy is a promising approach for tissue regeneration but there exists a problem of low engraftment rate to the injury site. Our laboratory has shown that hMSCs that were capable to penetrate through collagen barrier have higher migration capacity and engraftment efficiency than those remained inside the collagen matrix and those in traditional 2D culture. These cells capable of penetrating through collagen barrier might be hopeful candidate for improving engraftment efficacy. Major processes of engraftment, such as transmigration through basement membrane and invasion to the site of injury, involve cell-extracellular matrix-interacting proteins. As matrix metalloproteinases (MMP) and integrins are the key players in these processes, MMP and integrin profiles of the hMSCs were studied In this study, we demonstrated that hMSCs that were capable of penetrating through the collagen barrier have distinctive MMP profile to traditional 2D culture. These cells secrete significantly higher amount of MMP-1 than 2D culture, but the amount of MMP-2 secreted is comparable to traditional 2D culture. On the other hand, MMP-9 and MMP-13 were below detection limit by ELISA in both groups. Moreover, we have investigated the subcellular localization of MMPs and integrins. The cells were seeded on dishes with or without ECM coatings. It was demonstrated that hMSCs capable of penetrating through collagen barrier exhibit higher amount of subcellular MT1-MMP and integrin 6271 on ECM coated dish. Moreover, these cells exhibit a prominent feature of perinuclear localization of MT1-MMP., whereas the level of subcellular MMP-2, integrin 65 and 6v73 is comparable to that in 2D culture. We have also investigated the stem properties of hMSCs penetrated through collagen barrier. These properties include proliferation capacity, self-renewal capacity and differentiation potential towards chondrogenic, osteogenic and adipogenic lineages. It has been demonstrated that these properties are not compromised for superior migratory activities.-
dc.languageeng-
dc.publisherThe University of Hong Kong (Pokfulam, Hong Kong)-
dc.relation.ispartofHKU Theses Online (HKUTO)-
dc.rightsThe author retains all proprietary rights, (such as patent rights) and the right to use in future works.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.source.urihttp://hub.hku.hk/bib/B47869720-
dc.subject.lcshStem cells.-
dc.subject.lcshMesenchyme.-
dc.titleCharacterization of hMSCs transmigrated through collagen barrier-
dc.typePG_Thesis-
dc.identifier.hkulb4786972-
dc.description.thesisnameMaster of Philosophy-
dc.description.thesislevelmaster's-
dc.description.thesisdisciplineMechanical Engineering-
dc.description.naturepublished_or_final_version-
dc.date.hkucongregation2012-

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