Article: Hypoxia impairs primordial germ cell migration in zebrafish (danio rerio) embryos

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Publisher Website: 10.1371/journal.pone.0024540
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PMID: 21931746

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Title	Hypoxia impairs primordial germ cell migration in zebrafish (danio rerio) embryos
Authors	Lo, KH2 Hui, MNY2 Yu, RMK3 Wu, RSS1 2 Cheng, SH2
Issue Date	2011
Publisher	Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation	Plos One, 2011, v. 6 n. 9 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0024540
Abstract	Background: As a global environmental concern, hypoxia is known to be associated with many biological and physiological impairments in aquatic ecosystems. Previous studies have mainly focused on the effect of hypoxia in adult animals. However, the effect of hypoxia and the underlying mechanism of how hypoxia affects embryonic development of aquatic animals remain unclear. Methodology/Principal Findings: In the current study, the effect of hypoxia on primordial germ cell (PGC) migration in zebrafish embryos was investigated. Hypoxic embryos showed PGC migration defect as indicated by the presence of mis-migrated ectopic PGCs. Insulin-like growth factor (IGF) signaling is required for embryonic germ line development. Using real-time PCR, we found that the mRNA expression levels of insulin-like growth factor binding protein (IGFBP-1), an inhibitor of IGF bioactivity, were significantly increased in hypoxic embryos. Morpholino knockdown of IGFBP-1 rescued the PGC migration defect phenotype in hypoxic embryos, suggesting the role of IGFBP-1 in inducing PGC mis-migration. Conclusions/Significance: This study provides novel evidence that hypoxia disrupts PGC migration during embryonic development in fish. IGF signaling is shown to be one of the possible mechanisms for the causal link between hypoxia and PGC migration. We propose that hypoxia causes PGC migration defect by inhibiting IGF signaling through the induction of IGFBP-1. © 2011 Lo et al.
ISSN	1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519
DOI	http://dx.doi.org/10.1371/journal.pone.0024540
PubMed Central ID	PMC3169607
References	References in Scopus

DC Field	Value
dc.contributor.author	Lo, KH
dc.contributor.author	Hui, MNY
dc.contributor.author	Yu, RMK
dc.contributor.author	Wu, RSS
dc.contributor.author	Cheng, SH
dc.date.accessioned	2012-08-28T01:30:50Z
dc.date.available	2012-08-28T01:30:50Z
dc.date.issued	2011
dc.description.abstract	Background: As a global environmental concern, hypoxia is known to be associated with many biological and physiological impairments in aquatic ecosystems. Previous studies have mainly focused on the effect of hypoxia in adult animals. However, the effect of hypoxia and the underlying mechanism of how hypoxia affects embryonic development of aquatic animals remain unclear. Methodology/Principal Findings: In the current study, the effect of hypoxia on primordial germ cell (PGC) migration in zebrafish embryos was investigated. Hypoxic embryos showed PGC migration defect as indicated by the presence of mis-migrated ectopic PGCs. Insulin-like growth factor (IGF) signaling is required for embryonic germ line development. Using real-time PCR, we found that the mRNA expression levels of insulin-like growth factor binding protein (IGFBP-1), an inhibitor of IGF bioactivity, were significantly increased in hypoxic embryos. Morpholino knockdown of IGFBP-1 rescued the PGC migration defect phenotype in hypoxic embryos, suggesting the role of IGFBP-1 in inducing PGC mis-migration. Conclusions/Significance: This study provides novel evidence that hypoxia disrupts PGC migration during embryonic development in fish. IGF signaling is shown to be one of the possible mechanisms for the causal link between hypoxia and PGC migration. We propose that hypoxia causes PGC migration defect by inhibiting IGF signaling through the induction of IGFBP-1. © 2011 Lo et al.
dc.description.nature	published_or_final_version
dc.identifier.citation	Plos One, 2011, v. 6 n. 9 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0024540
dc.identifier.doi	http://dx.doi.org/10.1371/journal.pone.0024540
dc.identifier.hkuros	203341
dc.identifier.hkuros	208958
dc.identifier.issn	1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519
dc.identifier.issue	9
dc.identifier.pmcid	PMC3169607
dc.identifier.pmid	21931746
dc.identifier.scopus	eid_2-s2.0-80052541174
dc.identifier.uri	http://hdl.handle.net/10722/161499
dc.identifier.volume	6
dc.language	eng
dc.publisher	Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
dc.publisher.place	United States
dc.relation.ispartof	PLoS ONE
dc.relation.references	References in Scopus
dc.rights	Creative Commons: Attribution 3.0 Hong Kong License
dc.subject.mesh	Gene Expression Profiling
dc.subject.mesh	Gene Expression Regulation, Developmental
dc.subject.mesh	Germ Cells - cytology
dc.subject.mesh	Insulin-Like Growth Factor Binding Protein 1 - metabolism
dc.subject.mesh	RNA, Messenger - metabolism
dc.title	Hypoxia impairs primordial germ cell migration in zebrafish (danio rerio) embryos
dc.type	Article

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Author Affiliations

The University of Hong Kong
City University of Hong Kong
University of Newcastle, Australia