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Article: Hypoxia impairs primordial germ cell migration in zebrafish (danio rerio) embryos

TitleHypoxia impairs primordial germ cell migration in zebrafish (danio rerio) embryos
Authors
Issue Date2011
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2011, v. 6 n. 9 How to Cite?
Abstract
Background: As a global environmental concern, hypoxia is known to be associated with many biological and physiological impairments in aquatic ecosystems. Previous studies have mainly focused on the effect of hypoxia in adult animals. However, the effect of hypoxia and the underlying mechanism of how hypoxia affects embryonic development of aquatic animals remain unclear. Methodology/Principal Findings: In the current study, the effect of hypoxia on primordial germ cell (PGC) migration in zebrafish embryos was investigated. Hypoxic embryos showed PGC migration defect as indicated by the presence of mis-migrated ectopic PGCs. Insulin-like growth factor (IGF) signaling is required for embryonic germ line development. Using real-time PCR, we found that the mRNA expression levels of insulin-like growth factor binding protein (IGFBP-1), an inhibitor of IGF bioactivity, were significantly increased in hypoxic embryos. Morpholino knockdown of IGFBP-1 rescued the PGC migration defect phenotype in hypoxic embryos, suggesting the role of IGFBP-1 in inducing PGC mis-migration. Conclusions/Significance: This study provides novel evidence that hypoxia disrupts PGC migration during embryonic development in fish. IGF signaling is shown to be one of the possible mechanisms for the causal link between hypoxia and PGC migration. We propose that hypoxia causes PGC migration defect by inhibiting IGF signaling through the induction of IGFBP-1. © 2011 Lo et al.
Persistent Identifierhttp://hdl.handle.net/10722/161499
ISSN
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
PubMed Central ID
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong
  3. University of Newcastle, Australia
DC FieldValueLanguage
dc.contributor.authorLo, KHen_HK
dc.contributor.authorHui, MNYen_HK
dc.contributor.authorYu, RMKen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorCheng, SHen_HK
dc.date.accessioned2012-08-28T01:30:50Z-
dc.date.available2012-08-28T01:30:50Z-
dc.date.issued2011en_HK
dc.identifier.citationPlos One, 2011, v. 6 n. 9en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/161499-
dc.description.abstractBackground: As a global environmental concern, hypoxia is known to be associated with many biological and physiological impairments in aquatic ecosystems. Previous studies have mainly focused on the effect of hypoxia in adult animals. However, the effect of hypoxia and the underlying mechanism of how hypoxia affects embryonic development of aquatic animals remain unclear. Methodology/Principal Findings: In the current study, the effect of hypoxia on primordial germ cell (PGC) migration in zebrafish embryos was investigated. Hypoxic embryos showed PGC migration defect as indicated by the presence of mis-migrated ectopic PGCs. Insulin-like growth factor (IGF) signaling is required for embryonic germ line development. Using real-time PCR, we found that the mRNA expression levels of insulin-like growth factor binding protein (IGFBP-1), an inhibitor of IGF bioactivity, were significantly increased in hypoxic embryos. Morpholino knockdown of IGFBP-1 rescued the PGC migration defect phenotype in hypoxic embryos, suggesting the role of IGFBP-1 in inducing PGC mis-migration. Conclusions/Significance: This study provides novel evidence that hypoxia disrupts PGC migration during embryonic development in fish. IGF signaling is shown to be one of the possible mechanisms for the causal link between hypoxia and PGC migration. We propose that hypoxia causes PGC migration defect by inhibiting IGF signaling through the induction of IGFBP-1. © 2011 Lo et al.en_HK
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshGene Expression Profiling-
dc.subject.meshGene Expression Regulation, Developmental-
dc.subject.meshGerm Cells - cytology-
dc.subject.meshInsulin-Like Growth Factor Binding Protein 1 - metabolism-
dc.subject.meshRNA, Messenger - metabolism-
dc.titleHypoxia impairs primordial germ cell migration in zebrafish (danio rerio) embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0024540en_HK
dc.identifier.pmid21931746-
dc.identifier.pmcidPMC3169607-
dc.identifier.scopuseid_2-s2.0-80052541174en_HK
dc.identifier.hkuros203341-
dc.identifier.hkuros208958-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052541174&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue9en_HK
dc.identifier.isiWOS:000294802800062-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLo, KH=51763883100en_HK
dc.identifier.scopusauthoridHui, MNY=17342146300en_HK
dc.identifier.scopusauthoridYu, RMK=9278574900en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridCheng, SH=7404684691en_HK

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