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Article: Fluorescence in situ hybridization of metaphase chromosomes in suspension

TitleFluorescence in situ hybridization of metaphase chromosomes in suspension
Authors
Issue Date2001
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09553002.asp
Citation
International Journal Of Radiation Biology, 2001, v. 77 n. 7, p. 787-795 How to Cite?
AbstractPurpose: To describe a new method for FISH analysis of metaphase chromosomes in suspension. Materials and methods: Metaphase chromosomes in suspension were isolated from a Chinese hamster x human hybrid cell line, 314-2 (1) Y, and a human cell line, GM 130B. During suspension hybridization, specific chromosomes were labeled from these two cell lines using either biotin-labeled human genomic DNA, a directly labeled human pancentromere DNA probe or a chromosome #1 locus-specific probe. Results: The method allows, for the first time, recovery of large numbers of isolated individual hybridized chromosomes with good morphology for both human x hamster hybrid and human cell lines. The results showed that 46-73% of the starting number of total chromosomes can be recovered after a FISH in suspension procedure. The well-preserved morphology of hybridized metaphase chromosomes allowed (1) rapid detection of individual human and hamster chromosome aberrations, (2) rapid counting of the painted human chromosomes and (3) fast, clear detection of chromosome region-specific probes. This method offers a new tool to assay chromosomes and DNA: it offers the possibility to develop new techniques for sorting chromosomes based on FISH signals, for early detection and screening of genetic diseases and for bulk measurement of both balanced or unbalanced chromosomal exchanges and rearrangements. Conclusion: The potential of the method described should facilitate fast, sensitive population monitoring, and increase sensitivity of the measurements in chromosome-based biodosimetry.
Persistent Identifierhttp://hdl.handle.net/10722/161497
ISSN
2015 Impact Factor: 1.779
2015 SCImago Journal Rankings: 0.734
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHe, Hen_HK
dc.contributor.authorDeng, Wen_HK
dc.contributor.authorCassel, MJen_HK
dc.contributor.authorLucas, JNen_HK
dc.date.accessioned2012-08-27T04:49:05Z-
dc.date.available2012-08-27T04:49:05Z-
dc.date.issued2001en_HK
dc.identifier.citationInternational Journal Of Radiation Biology, 2001, v. 77 n. 7, p. 787-795en_HK
dc.identifier.issn0955-3002en_HK
dc.identifier.urihttp://hdl.handle.net/10722/161497-
dc.description.abstractPurpose: To describe a new method for FISH analysis of metaphase chromosomes in suspension. Materials and methods: Metaphase chromosomes in suspension were isolated from a Chinese hamster x human hybrid cell line, 314-2 (1) Y, and a human cell line, GM 130B. During suspension hybridization, specific chromosomes were labeled from these two cell lines using either biotin-labeled human genomic DNA, a directly labeled human pancentromere DNA probe or a chromosome #1 locus-specific probe. Results: The method allows, for the first time, recovery of large numbers of isolated individual hybridized chromosomes with good morphology for both human x hamster hybrid and human cell lines. The results showed that 46-73% of the starting number of total chromosomes can be recovered after a FISH in suspension procedure. The well-preserved morphology of hybridized metaphase chromosomes allowed (1) rapid detection of individual human and hamster chromosome aberrations, (2) rapid counting of the painted human chromosomes and (3) fast, clear detection of chromosome region-specific probes. This method offers a new tool to assay chromosomes and DNA: it offers the possibility to develop new techniques for sorting chromosomes based on FISH signals, for early detection and screening of genetic diseases and for bulk measurement of both balanced or unbalanced chromosomal exchanges and rearrangements. Conclusion: The potential of the method described should facilitate fast, sensitive population monitoring, and increase sensitivity of the measurements in chromosome-based biodosimetry.en_HK
dc.languageeng-
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/09553002.aspen_HK
dc.relation.ispartofInternational Journal of Radiation Biologyen_HK
dc.rightsInternational Journal of Radiation Biology . Copyright © Informa Healthcare.-
dc.subject.meshChromosomes - ultrastructure-
dc.subject.meshDNA - ultrastructure-
dc.subject.meshIn Situ Hybridization-
dc.subject.meshIn Situ Hybridization, Fluorescence - methods-
dc.subject.meshMetaphase-
dc.titleFluorescence in situ hybridization of metaphase chromosomes in suspensionen_HK
dc.typeArticleen_HK
dc.identifier.emailDeng, W: wdeng@hkucc.hku.hken_HK
dc.identifier.authorityDeng, W=rp01640en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/09553000110047546en_HK
dc.identifier.pmid11454279-
dc.identifier.scopuseid_2-s2.0-0034930755en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034930755&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume77en_HK
dc.identifier.issue7en_HK
dc.identifier.spage787en_HK
dc.identifier.epage795en_HK
dc.identifier.isiWOS:000169751700004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHe, H=7402291509en_HK
dc.identifier.scopusauthoridDeng, W=7202223673en_HK
dc.identifier.scopusauthoridCassel, MJ=55376258300en_HK
dc.identifier.scopusauthoridLucas, JN=7402441937en_HK

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