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Article: Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses

TitlePlatycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses
Authors
Issue Date2008
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/intimp
Citation
International Immunopharmacology, 2008, v. 8 n. 8, p. 1143-1150 How to Cite?
AbstractPlatycodin D2 (PD2), a saponin isolated from the root of Platycodon grandiflorum, was evaluated for its haemolytic activity and adjuvant potential on the cellular and humoral immune responses to ovalbumin (OVA) in ICR mice. The HD 50 values of PD2 and Quil A were 18.57 ± 1.37 and 5.76 ± 0.23 μg/ml against 0.5% rabbit red blood cell, respectively, implicating the less haemolytic activity for PD2 than Quil A. ICR mice were immunized subcutaneously with OVA 100 μg alone or with OVA 100 μg dissolved in saline containing Alum (200 μg), or PD2 (25, 50, 75 or 100 μg) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS-) and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. PD2 significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice (P < 0.05, P < 0.01 or P < 0.001). OVA-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in serum were also significantly enhanced by PD2 compared with OVA control group (P < 0.05, P < 0.01 or P < 0.001). Further, the effects of PD2 on mRNA expression of cytokines and transcription factors in Con A-stimulated mice splenocytes were evaluated by RT-PCR analysis. PD2 significantly promoted the mRNA expression of cytokines IL-2, IFN-γ, IL-4, and IL-10 and transcription factors T-bet and GATA-3 (P < 0.05, P < 0.01 or P < 0.001). In conclusion, the results suggest that PD2 could be safely used as adjuvant eliciting Th1 and Th2 immune responses. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/161283
ISSN
2015 Impact Factor: 2.551
2015 SCImago Journal Rankings: 0.941
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXie, Yen_US
dc.contributor.authorDeng, Wen_US
dc.contributor.authorSun, Hen_US
dc.contributor.authorLi, Den_US
dc.date.accessioned2012-08-24T08:29:25Z-
dc.date.available2012-08-24T08:29:25Z-
dc.date.issued2008en_US
dc.identifier.citationInternational Immunopharmacology, 2008, v. 8 n. 8, p. 1143-1150en_US
dc.identifier.issn1567-5769en_US
dc.identifier.urihttp://hdl.handle.net/10722/161283-
dc.description.abstractPlatycodin D2 (PD2), a saponin isolated from the root of Platycodon grandiflorum, was evaluated for its haemolytic activity and adjuvant potential on the cellular and humoral immune responses to ovalbumin (OVA) in ICR mice. The HD 50 values of PD2 and Quil A were 18.57 ± 1.37 and 5.76 ± 0.23 μg/ml against 0.5% rabbit red blood cell, respectively, implicating the less haemolytic activity for PD2 than Quil A. ICR mice were immunized subcutaneously with OVA 100 μg alone or with OVA 100 μg dissolved in saline containing Alum (200 μg), or PD2 (25, 50, 75 or 100 μg) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS-) and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. PD2 significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice (P < 0.05, P < 0.01 or P < 0.001). OVA-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in serum were also significantly enhanced by PD2 compared with OVA control group (P < 0.05, P < 0.01 or P < 0.001). Further, the effects of PD2 on mRNA expression of cytokines and transcription factors in Con A-stimulated mice splenocytes were evaluated by RT-PCR analysis. PD2 significantly promoted the mRNA expression of cytokines IL-2, IFN-γ, IL-4, and IL-10 and transcription factors T-bet and GATA-3 (P < 0.05, P < 0.01 or P < 0.001). In conclusion, the results suggest that PD2 could be safely used as adjuvant eliciting Th1 and Th2 immune responses. © 2008 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/intimpen_US
dc.relation.ispartofInternational Immunopharmacologyen_US
dc.subject.meshAdjuvants, Immunologicen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies - Blooden_US
dc.subject.meshAntibody Formation - Drug Effectsen_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshCytokines - Immunology - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshHemolysisen_US
dc.subject.meshImmunity, Cellular - Drug Effectsen_US
dc.subject.meshImmunizationen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshMiceen_US
dc.subject.meshOvalbumin - Immunology - Metabolismen_US
dc.subject.meshSaponins - Chemistry - Immunology - Isolation & Purification - Pharmacologyen_US
dc.subject.meshTh1 Cells - Drug Effects - Immunology - Metabolismen_US
dc.subject.meshTh2 Cells - Drug Effects - Immunology - Metabolismen_US
dc.subject.meshTranscription Factors - Metabolismen_US
dc.subject.meshTriterpenes - Chemistry - Immunology - Isolation & Purification - Pharmacologyen_US
dc.titlePlatycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responsesen_US
dc.typeArticleen_US
dc.identifier.emailDeng, W:wdeng@hkucc.hku.hken_US
dc.identifier.authorityDeng, W=rp01640en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.intimp.2008.04.006en_US
dc.identifier.pmid18550019-
dc.identifier.scopuseid_2-s2.0-50949087401en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-50949087401&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume8en_US
dc.identifier.issue8en_US
dc.identifier.spage1143en_US
dc.identifier.epage1150en_US
dc.identifier.isiWOS:000257577000011-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridXie, Y=35199376800en_US
dc.identifier.scopusauthoridDeng, W=55160364700en_US
dc.identifier.scopusauthoridSun, H=7404827412en_US
dc.identifier.scopusauthoridLi, D=8154232600en_US

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