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Article: In vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii
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TitleIn vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii
 
AuthorsYang, X5
Huang, B5
Chen, J2
Huang, S6
Zheng, H5
Lun, ZR1 5
Shen, J3
Wang, Y4
Lu, F5
 
Issue Date2012
 
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00436/index.htm
 
CitationParasitology Research, 2012, v. 110 n. 6, p. 2221-2227 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00436-011-2752-2
 
AbstractToxoplasma gondii is a parasite that infects animals and humans worldwide. The standard treatment for toxoplasmosis is limiting due to toxic adverse effects, thus there is a need to identify new drugs that are less toxic. Both Astragalus membranaceus and Scutellaria baicalensis GEORGI are popular traditional Chinese herbs widely used for the treatment of various inflammatory diseases in Asia, and we have previously demonstrated that water extracts of A. membranaceus (AmE) and S. baicalensis GEORGI (SbE) have good efficacy in controlling T. gondii replication in mouse models. This study was designed to further evaluate their effects against developing tachyzoites of the RH strain of T. gondii in HeLa cell cultures. AmE, SbE, and TMP-SMX (trimethoprim- sulfamethoxazole) were added into the wells containing both HeLa cells and replicating T. gondii of green fluorescent protein (GFP)-expressing RH tachyzoites. The proliferation and morphous of the tachyzoites were observed, the fluorescence intensity expressed as the fluorescence gray scale value was measured, and the living tachyzoites were counted at different culture times after treatment. The results showed that, compared to untreated controls, parasites treated with either AmE or SbE had significantly decreased intracellular replication at 72, 96, and 120 h after treatment (P<0.01); while compared to either AmE- or SbE-treated groups, SMX-treated groups had even significantly decreased replication (only a few living parasites were detected) at the above times (P<0.01). Our data demonstrated that both AmE and SbE had remarkable in vitro activities against T. gondii. © Springer-Verlag 2011.
 
ISSN0932-0113
2013 Impact Factor: 2.327
 
DOIhttp://dx.doi.org/10.1007/s00436-011-2752-2
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYang, X
 
dc.contributor.authorHuang, B
 
dc.contributor.authorChen, J
 
dc.contributor.authorHuang, S
 
dc.contributor.authorZheng, H
 
dc.contributor.authorLun, ZR
 
dc.contributor.authorShen, J
 
dc.contributor.authorWang, Y
 
dc.contributor.authorLu, F
 
dc.date.accessioned2012-08-16T06:17:20Z
 
dc.date.available2012-08-16T06:17:20Z
 
dc.date.issued2012
 
dc.description.abstractToxoplasma gondii is a parasite that infects animals and humans worldwide. The standard treatment for toxoplasmosis is limiting due to toxic adverse effects, thus there is a need to identify new drugs that are less toxic. Both Astragalus membranaceus and Scutellaria baicalensis GEORGI are popular traditional Chinese herbs widely used for the treatment of various inflammatory diseases in Asia, and we have previously demonstrated that water extracts of A. membranaceus (AmE) and S. baicalensis GEORGI (SbE) have good efficacy in controlling T. gondii replication in mouse models. This study was designed to further evaluate their effects against developing tachyzoites of the RH strain of T. gondii in HeLa cell cultures. AmE, SbE, and TMP-SMX (trimethoprim- sulfamethoxazole) were added into the wells containing both HeLa cells and replicating T. gondii of green fluorescent protein (GFP)-expressing RH tachyzoites. The proliferation and morphous of the tachyzoites were observed, the fluorescence intensity expressed as the fluorescence gray scale value was measured, and the living tachyzoites were counted at different culture times after treatment. The results showed that, compared to untreated controls, parasites treated with either AmE or SbE had significantly decreased intracellular replication at 72, 96, and 120 h after treatment (P<0.01); while compared to either AmE- or SbE-treated groups, SMX-treated groups had even significantly decreased replication (only a few living parasites were detected) at the above times (P<0.01). Our data demonstrated that both AmE and SbE had remarkable in vitro activities against T. gondii. © Springer-Verlag 2011.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationParasitology Research, 2012, v. 110 n. 6, p. 2221-2227 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00436-011-2752-2
 
dc.identifier.citeulike10149672
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00436-011-2752-2
 
dc.identifier.epage2227
 
dc.identifier.hkuros203867
 
dc.identifier.issn0932-0113
2013 Impact Factor: 2.327
 
dc.identifier.issue6
 
dc.identifier.pmid22179265
 
dc.identifier.scopuseid_2-s2.0-84863427851
 
dc.identifier.spage2221
 
dc.identifier.urihttp://hdl.handle.net/10722/160721
 
dc.identifier.volume110
 
dc.languageeng
 
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00436/index.htm
 
dc.publisher.placeGermany
 
dc.relation.ispartofParasitology Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAntiprotozoal Agents - isolation & purification - pharmacology
 
dc.subject.meshAstragalus membranaceus - chemistry
 
dc.subject.meshEpithelial Cells - parasitology
 
dc.subject.meshHeLa Cells
 
dc.subject.meshHumans
 
dc.subject.meshPlant Extracts - isolation & purification - pharmacology
 
dc.subject.meshScutellaria baicalensis - chemistry
 
dc.subject.meshTime Factors
 
dc.subject.meshToxoplasma - drug effects - growth & development
 
dc.titleIn vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii
 
dc.typeArticle
 
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<contributor.author>Zheng, H</contributor.author>
<contributor.author>Lun, ZR</contributor.author>
<contributor.author>Shen, J</contributor.author>
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Author Affiliations
  1. Fudan University School of Life Sciences
  2. The University of Hong Kong
  3. Anhui Medical University
  4. Nanjing Medical University
  5. Sun Yat-Sen University
  6. Jinan University