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Article: In vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii

TitleIn vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondii
Authors
Issue Date2012
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00436/index.htm
Citation
Parasitology Research, 2012, v. 110 n. 6, p. 2221-2227 How to Cite?
AbstractToxoplasma gondii is a parasite that infects animals and humans worldwide. The standard treatment for toxoplasmosis is limiting due to toxic adverse effects, thus there is a need to identify new drugs that are less toxic. Both Astragalus membranaceus and Scutellaria baicalensis GEORGI are popular traditional Chinese herbs widely used for the treatment of various inflammatory diseases in Asia, and we have previously demonstrated that water extracts of A. membranaceus (AmE) and S. baicalensis GEORGI (SbE) have good efficacy in controlling T. gondii replication in mouse models. This study was designed to further evaluate their effects against developing tachyzoites of the RH strain of T. gondii in HeLa cell cultures. AmE, SbE, and TMP-SMX (trimethoprim- sulfamethoxazole) were added into the wells containing both HeLa cells and replicating T. gondii of green fluorescent protein (GFP)-expressing RH tachyzoites. The proliferation and morphous of the tachyzoites were observed, the fluorescence intensity expressed as the fluorescence gray scale value was measured, and the living tachyzoites were counted at different culture times after treatment. The results showed that, compared to untreated controls, parasites treated with either AmE or SbE had significantly decreased intracellular replication at 72, 96, and 120 h after treatment (P<0.01); while compared to either AmE- or SbE-treated groups, SMX-treated groups had even significantly decreased replication (only a few living parasites were detected) at the above times (P<0.01). Our data demonstrated that both AmE and SbE had remarkable in vitro activities against T. gondii. © Springer-Verlag 2011.
Persistent Identifierhttp://hdl.handle.net/10722/160721
ISSN
2014 Impact Factor: 2.098
2014 SCImago Journal Rankings: 0.886
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Xen_HK
dc.contributor.authorHuang, Ben_HK
dc.contributor.authorChen, Jen_HK
dc.contributor.authorHuang, Sen_HK
dc.contributor.authorZheng, Hen_HK
dc.contributor.authorLun, ZRen_HK
dc.contributor.authorShen, Jen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLu, Fen_HK
dc.date.accessioned2012-08-16T06:17:20Z-
dc.date.available2012-08-16T06:17:20Z-
dc.date.issued2012en_HK
dc.identifier.citationParasitology Research, 2012, v. 110 n. 6, p. 2221-2227en_HK
dc.identifier.issn0932-0113en_HK
dc.identifier.urihttp://hdl.handle.net/10722/160721-
dc.description.abstractToxoplasma gondii is a parasite that infects animals and humans worldwide. The standard treatment for toxoplasmosis is limiting due to toxic adverse effects, thus there is a need to identify new drugs that are less toxic. Both Astragalus membranaceus and Scutellaria baicalensis GEORGI are popular traditional Chinese herbs widely used for the treatment of various inflammatory diseases in Asia, and we have previously demonstrated that water extracts of A. membranaceus (AmE) and S. baicalensis GEORGI (SbE) have good efficacy in controlling T. gondii replication in mouse models. This study was designed to further evaluate their effects against developing tachyzoites of the RH strain of T. gondii in HeLa cell cultures. AmE, SbE, and TMP-SMX (trimethoprim- sulfamethoxazole) were added into the wells containing both HeLa cells and replicating T. gondii of green fluorescent protein (GFP)-expressing RH tachyzoites. The proliferation and morphous of the tachyzoites were observed, the fluorescence intensity expressed as the fluorescence gray scale value was measured, and the living tachyzoites were counted at different culture times after treatment. The results showed that, compared to untreated controls, parasites treated with either AmE or SbE had significantly decreased intracellular replication at 72, 96, and 120 h after treatment (P<0.01); while compared to either AmE- or SbE-treated groups, SMX-treated groups had even significantly decreased replication (only a few living parasites were detected) at the above times (P<0.01). Our data demonstrated that both AmE and SbE had remarkable in vitro activities against T. gondii. © Springer-Verlag 2011.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00436/index.htmen_HK
dc.relation.ispartofParasitology Researchen_HK
dc.subject.meshAntiprotozoal Agents - isolation & purification - pharmacologyen_HK
dc.subject.meshAstragalus membranaceus - chemistryen_HK
dc.subject.meshEpithelial Cells - parasitologyen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPlant Extracts - isolation & purification - pharmacologyen_HK
dc.subject.meshScutellaria baicalensis - chemistryen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshToxoplasma - drug effects - growth & developmenten_HK
dc.titleIn vitro effects of aqueous extracts of Astragalus membranaceus and Scutellaria baicalensis GEORGI on Toxoplasma gondiien_HK
dc.typeArticleen_HK
dc.identifier.emailChen, J: abchen@hku.hken_HK
dc.identifier.authorityChen, J=rp01316en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00436-011-2752-2en_HK
dc.identifier.pmid22179265en_HK
dc.identifier.scopuseid_2-s2.0-84863427851en_HK
dc.identifier.hkuros203867en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863427851&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume110en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2221en_HK
dc.identifier.epage2227en_HK
dc.identifier.isiWOS:000304627300018-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridYang, X=22952286800en_HK
dc.identifier.scopusauthoridHuang, B=34769825900en_HK
dc.identifier.scopusauthoridChen, J=22733695400en_HK
dc.identifier.scopusauthoridHuang, S=7405422703en_HK
dc.identifier.scopusauthoridZheng, H=7403440550en_HK
dc.identifier.scopusauthoridLun, ZR=7003966590en_HK
dc.identifier.scopusauthoridShen, J=7404930725en_HK
dc.identifier.scopusauthoridWang, Y=36072381800en_HK
dc.identifier.scopusauthoridLu, F=7402968086en_HK
dc.identifier.citeulike10149672-

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