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Article: Concomitant induction of heme oxygenase-1 attenuates the cytotoxicity of arsenic species from Lumbricus extract in human liver HepG2 cells

TitleConcomitant induction of heme oxygenase-1 attenuates the cytotoxicity of arsenic species from Lumbricus extract in human liver HepG2 cells
Authors
Issue Date2012
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/106056929
Citation
Chemistry And Biodiversity, 2012, v. 9 n. 4, p. 739-754 How to Cite?
Abstract
Heme oxygenase-1 (HO-1) is an inducible antioxidant enzyme that degrades heme to three products, biliverdin, carbon monoxide (CO), and iron ion. The present study was originally designed to characterize the HO-1 induction by Lumbricus extract as a potential cytoprotective mechanism. Through bioactivity-guided fractionation, with human HepG2 cells as the cellular detector, surprisingly, we found that arsenic was enriched in the active fractions isolated from Lumbricus extract. Arsenic speciation was further carried out by liquid chromatography with inductively coupled plasma mass spectrometry (LC/ ICP-MS). Our results showed that Lumbricus extract contained two major arsenic species, arsenite (As III; 53.7%) and arsenate (As V; 34.2%), and six minor arsenic species. Commercial sodium arsenite (NaAsO 2) was used to verify the effects of Lumbricus extract on HO-1 expression and related intracellular signaling pathways. Both p38MAP kinase and NF-E2-related factor 2 (Nrf2) pathways were found to modulate HO-1 induction by Lumbricus extract and NaAsO 2. The cytotoxicity of arsenite was augmented by p38 MAP kinase inhibitor SB202190 and HO-1 inhibitor tin protoporphyrin IX (SnPP), whereas p38 MAP kinase inhibitor SB202190 also inhibited HO-1 induction by NaAsO 2. These results suggest that arsenic-containing compounds are responsible for HO-1 induction by Lumbricus extract. Although the exact role of toxic arsenic compounds in the treatment of oxidative injury remains unclear, concomitant HO-1 induction may be a key mechanism to antagonize the cytotoxicity of arsenic compounds in human cells. © 2012 Verlag Helvetica Chimica Acta AG.
Persistent Identifierhttp://hdl.handle.net/10722/160718
ISSN
2013 Impact Factor: 1.795
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. University of Alberta
DC FieldValueLanguage
dc.contributor.authorQi, Hen_HK
dc.contributor.authorChen, Ben_HK
dc.contributor.authorLe, XCen_HK
dc.contributor.authorRong, Jen_HK
dc.date.accessioned2012-08-16T06:17:18Z-
dc.date.available2012-08-16T06:17:18Z-
dc.date.issued2012en_HK
dc.identifier.citationChemistry And Biodiversity, 2012, v. 9 n. 4, p. 739-754en_HK
dc.identifier.issn1612-1872en_HK
dc.identifier.urihttp://hdl.handle.net/10722/160718-
dc.description.abstractHeme oxygenase-1 (HO-1) is an inducible antioxidant enzyme that degrades heme to three products, biliverdin, carbon monoxide (CO), and iron ion. The present study was originally designed to characterize the HO-1 induction by Lumbricus extract as a potential cytoprotective mechanism. Through bioactivity-guided fractionation, with human HepG2 cells as the cellular detector, surprisingly, we found that arsenic was enriched in the active fractions isolated from Lumbricus extract. Arsenic speciation was further carried out by liquid chromatography with inductively coupled plasma mass spectrometry (LC/ ICP-MS). Our results showed that Lumbricus extract contained two major arsenic species, arsenite (As III; 53.7%) and arsenate (As V; 34.2%), and six minor arsenic species. Commercial sodium arsenite (NaAsO 2) was used to verify the effects of Lumbricus extract on HO-1 expression and related intracellular signaling pathways. Both p38MAP kinase and NF-E2-related factor 2 (Nrf2) pathways were found to modulate HO-1 induction by Lumbricus extract and NaAsO 2. The cytotoxicity of arsenite was augmented by p38 MAP kinase inhibitor SB202190 and HO-1 inhibitor tin protoporphyrin IX (SnPP), whereas p38 MAP kinase inhibitor SB202190 also inhibited HO-1 induction by NaAsO 2. These results suggest that arsenic-containing compounds are responsible for HO-1 induction by Lumbricus extract. Although the exact role of toxic arsenic compounds in the treatment of oxidative injury remains unclear, concomitant HO-1 induction may be a key mechanism to antagonize the cytotoxicity of arsenic compounds in human cells. © 2012 Verlag Helvetica Chimica Acta AG.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/106056929en_HK
dc.relation.ispartofChemistry and Biodiversityen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntineoplastic Agents - chemistry - isolation & purification - pharmacologyen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshArsenicals - chemistry - isolation & purification - pharmacologyen_HK
dc.subject.meshHeme Oxygenase-1 - metabolismen_HK
dc.subject.meshHep G2 Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshNeoplasms - drug therapy - enzymologyen_HK
dc.subject.meshOligochaeta - chemistryen_HK
dc.subject.meshSignal Transduction - drug effectsen_HK
dc.titleConcomitant induction of heme oxygenase-1 attenuates the cytotoxicity of arsenic species from Lumbricus extract in human liver HepG2 cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailRong, J: jrong@hku.hken_HK
dc.identifier.authorityRong, J=rp00515en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cbdv.201100133en_HK
dc.identifier.pmid22492492en_HK
dc.identifier.scopuseid_2-s2.0-84859794329en_HK
dc.identifier.hkuros203762en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84859794329&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue4en_HK
dc.identifier.spage739en_HK
dc.identifier.epage754en_HK
dc.identifier.isiWOS:000302617500007-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridQi, H=35367105300en_HK
dc.identifier.scopusauthoridChen, B=7408609812en_HK
dc.identifier.scopusauthoridLe, XC=7007054458en_HK
dc.identifier.scopusauthoridRong, J=7005980047en_HK

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