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Article: An inverse relationship to germline transcription defines centromeric chromatin in C. elegans

TitleAn inverse relationship to germline transcription defines centromeric chromatin in C. elegans
Authors
Issue Date2012
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nature
Citation
Nature, 2012, v. 484 n. 7395, p. 534-537 How to Cite?
AbstractCentromeres are chromosomal loci that direct segregation of the genome during cell division. The histone H3 variant CENP-A (also known as CenH3) defines centromeres in monocentric organisms, which confine centromere activity to a discrete chromosomal region, and holocentric organisms, which distribute centromere activity along the chromosome length. Because the highly repetitive DNA found at most centromeres is neither necessary nor sufficient for centromere function, stable inheritance of CENP-A nucleosomal chromatin is postulated to propagate centromere identity epigenetically. Here, we show that in the holocentric nematode Caenorhabditis elegans pre-existing CENP-A nucleosomes are not necessary to guide recruitment of new CENP-A nucleosomes. This is indicated by lack of CENP-A transmission by sperm during fertilization and by removal and subsequent reloading of CENP-A during oogenic meiotic prophase. Genome-wide mapping of CENP-A location in embryos and quantification of CENP-A molecules in nuclei revealed that CENP-A is incorporated at low density in domains that cumulatively encompass half the genome. Embryonic CENP-A domains are established in a pattern inverse to regions that are transcribed in the germline and early embryo, and ectopic transcription of genes in a mutant germline altered the pattern of CENP-A incorporation in embryos. Furthermore, regions transcribed in the germline but not embryos fail to incorporate CENP-A throughout embryogenesis. We propose that germline transcription defines genomic regions that exclude CENP-A incorporation in progeny, and that zygotic transcription during early embryogenesis remodels and reinforces this basal pattern. These findings link centromere identity to transcription and shed light on the evolutionary plasticity of centromeres. © 2012 Macmillan Publishers Limited. All rights reserved.
DescriptionLetter
Persistent Identifierhttp://hdl.handle.net/10722/160567
ISSN
2015 Impact Factor: 38.138
2015 SCImago Journal Rankings: 21.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGassmann, Ren_HK
dc.contributor.authorRechtsteiner, Aen_HK
dc.contributor.authorYuen, KWen_HK
dc.contributor.authorMuroyama, Aen_HK
dc.contributor.authorEgelhofer, Ten_HK
dc.contributor.authorGaydos, Len_HK
dc.contributor.authorBarron, Fen_HK
dc.contributor.authorMaddox, Pen_HK
dc.contributor.authorEssex, Aen_HK
dc.contributor.authorMonen, Jen_HK
dc.contributor.authorErcan, Sen_HK
dc.contributor.authorLieb, JDen_HK
dc.contributor.authorOegema, Ken_HK
dc.contributor.authorStrome, Sen_HK
dc.contributor.authorDesai, Aen_HK
dc.date.accessioned2012-08-16T06:14:41Z-
dc.date.available2012-08-16T06:14:41Z-
dc.date.issued2012en_HK
dc.identifier.citationNature, 2012, v. 484 n. 7395, p. 534-537en_HK
dc.identifier.issn0028-0836en_HK
dc.identifier.urihttp://hdl.handle.net/10722/160567-
dc.descriptionLetter-
dc.description.abstractCentromeres are chromosomal loci that direct segregation of the genome during cell division. The histone H3 variant CENP-A (also known as CenH3) defines centromeres in monocentric organisms, which confine centromere activity to a discrete chromosomal region, and holocentric organisms, which distribute centromere activity along the chromosome length. Because the highly repetitive DNA found at most centromeres is neither necessary nor sufficient for centromere function, stable inheritance of CENP-A nucleosomal chromatin is postulated to propagate centromere identity epigenetically. Here, we show that in the holocentric nematode Caenorhabditis elegans pre-existing CENP-A nucleosomes are not necessary to guide recruitment of new CENP-A nucleosomes. This is indicated by lack of CENP-A transmission by sperm during fertilization and by removal and subsequent reloading of CENP-A during oogenic meiotic prophase. Genome-wide mapping of CENP-A location in embryos and quantification of CENP-A molecules in nuclei revealed that CENP-A is incorporated at low density in domains that cumulatively encompass half the genome. Embryonic CENP-A domains are established in a pattern inverse to regions that are transcribed in the germline and early embryo, and ectopic transcription of genes in a mutant germline altered the pattern of CENP-A incorporation in embryos. Furthermore, regions transcribed in the germline but not embryos fail to incorporate CENP-A throughout embryogenesis. We propose that germline transcription defines genomic regions that exclude CENP-A incorporation in progeny, and that zygotic transcription during early embryogenesis remodels and reinforces this basal pattern. These findings link centromere identity to transcription and shed light on the evolutionary plasticity of centromeres. © 2012 Macmillan Publishers Limited. All rights reserved.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/natureen_HK
dc.relation.ispartofNatureen_HK
dc.subject.meshCaenorhabditis elegans - embryology - genetics-
dc.subject.meshCentromere - genetics-
dc.subject.meshChromatin - genetics-
dc.subject.meshChromosomal Proteins, Non-Histone - metabolism-
dc.subject.meshTranscription, Genetic-
dc.titleAn inverse relationship to germline transcription defines centromeric chromatin in C. elegansen_HK
dc.typeArticleen_HK
dc.identifier.emailYuen, KW: kwyyuen@hku.hken_HK
dc.identifier.authorityYuen, KW=rp01512en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/nature10973en_HK
dc.identifier.pmid22495302-
dc.identifier.scopuseid_2-s2.0-84860253153en_HK
dc.identifier.hkuros202275en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84860253153&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume484en_HK
dc.identifier.issue7395en_HK
dc.identifier.spage534en_HK
dc.identifier.epage537en_HK
dc.identifier.isiWOS:000303200400055-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.f100014267086-
dc.identifier.scopusauthoridGassmann, R=7003427025en_HK
dc.identifier.scopusauthoridRechtsteiner, A=14625456600en_HK
dc.identifier.scopusauthoridYuen, KW=8841935800en_HK
dc.identifier.scopusauthoridMuroyama, A=37031589300en_HK
dc.identifier.scopusauthoridEgelhofer, T=16300824900en_HK
dc.identifier.scopusauthoridGaydos, L=55178003000en_HK
dc.identifier.scopusauthoridBarron, F=37861033500en_HK
dc.identifier.scopusauthoridMaddox, P=7006625604en_HK
dc.identifier.scopusauthoridEssex, A=6506795017en_HK
dc.identifier.scopusauthoridMonen, J=9740039100en_HK
dc.identifier.scopusauthoridErcan, S=14719493400en_HK
dc.identifier.scopusauthoridLieb, JD=7005815585en_HK
dc.identifier.scopusauthoridOegema, K=6603708915en_HK
dc.identifier.scopusauthoridStrome, S=7004882319en_HK
dc.identifier.scopusauthoridDesai, A=7201793131en_HK
dc.identifier.citeulike11920809-

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