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Conference Paper: Protective effects of lycium barbarum polysaccharides on cerebral edema and blood-brain barrier disruption after ischemic stroke
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TitleProtective effects of lycium barbarum polysaccharides on cerebral edema and blood-brain barrier disruption after ischemic stroke
 
AuthorsYang, FD
Li, SY
Yeung, CM
Chang, RCC
So, KF
Wong, D
Lo, ACY
 
Issue Date2012
 
PublisherHKSN & BPHK.
 
CitationThe Joint Annual Scientific Meeting of Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book, 2012, p. 41 [How to Cite?]
 
AbstractBACKGROUND: Ischemic stroke is a destructive cerebrovascular disease and one of the leading causes of death worldwide. The long term disability after stroke induces heavy burden both to the patients and the society. Yet, no effective neuroprotective agents are available. The polysaccharides extracted from the fruits of wolfberry, Lycium barbarum (LBP), showed neuroprotective and immune-modulative functions. We aim to evaluate the protective effects of LBP in experimental stroke using a focal cerebral ischemia/reperfusion (I/R) model. METHODS: C57BL/6N mice were subjected to 2 h of middle cerebral artery occlusion (MCAO) followed by 22 h of reperfusion. Prior to ischemia induction, animals were treated with either vehicle (PBS) or LBP daily for 7 days. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement and immunohistochemical analysis as well as Western blot experiments. Evans blue (EB) extravasation experiment was performed to determine blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP treatment significantly improved neurological scores and decreased infarct size, hemispheric swelling and water content as well as reduced EB extravasation. In addition, fewer apoptotic cells were identified in the LBP-treated brains by TUNEL assay. Immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were also significantly decreased in LBP-treated brains. We further observed a reduction of nuclear factor-κB translocation and IκB expression after LBP treatment. CONCLUSION: Seven-day LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin water channel up-regulation and glial activation. The protective effects of LBP might partially act through its anti-inflammatory effects. The present study suggests that LBP may be used as a preventive neuroprotectant for ischemic stroke.
 
DescriptionYoung Investigators Symposium I (Y3) - Di Yang
 
DC FieldValue
dc.contributor.authorYang, FD
 
dc.contributor.authorLi, SY
 
dc.contributor.authorYeung, CM
 
dc.contributor.authorChang, RCC
 
dc.contributor.authorSo, KF
 
dc.contributor.authorWong, D
 
dc.contributor.authorLo, ACY
 
dc.date.accessioned2012-08-16T06:04:10Z
 
dc.date.available2012-08-16T06:04:10Z
 
dc.date.issued2012
 
dc.description.abstractBACKGROUND: Ischemic stroke is a destructive cerebrovascular disease and one of the leading causes of death worldwide. The long term disability after stroke induces heavy burden both to the patients and the society. Yet, no effective neuroprotective agents are available. The polysaccharides extracted from the fruits of wolfberry, Lycium barbarum (LBP), showed neuroprotective and immune-modulative functions. We aim to evaluate the protective effects of LBP in experimental stroke using a focal cerebral ischemia/reperfusion (I/R) model. METHODS: C57BL/6N mice were subjected to 2 h of middle cerebral artery occlusion (MCAO) followed by 22 h of reperfusion. Prior to ischemia induction, animals were treated with either vehicle (PBS) or LBP daily for 7 days. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement and immunohistochemical analysis as well as Western blot experiments. Evans blue (EB) extravasation experiment was performed to determine blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP treatment significantly improved neurological scores and decreased infarct size, hemispheric swelling and water content as well as reduced EB extravasation. In addition, fewer apoptotic cells were identified in the LBP-treated brains by TUNEL assay. Immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were also significantly decreased in LBP-treated brains. We further observed a reduction of nuclear factor-κB translocation and IκB expression after LBP treatment. CONCLUSION: Seven-day LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin water channel up-regulation and glial activation. The protective effects of LBP might partially act through its anti-inflammatory effects. The present study suggests that LBP may be used as a preventive neuroprotectant for ischemic stroke.
 
dc.description.naturepostprint
 
dc.descriptionYoung Investigators Symposium I (Y3) - Di Yang
 
dc.identifier.citationThe Joint Annual Scientific Meeting of Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book, 2012, p. 41 [How to Cite?]
 
dc.identifier.epage41
 
dc.identifier.hkuros205743
 
dc.identifier.spage41
 
dc.identifier.urihttp://hdl.handle.net/10722/160136
 
dc.languageeng
 
dc.publisherHKSN & BPHK.
 
dc.publisher.placeHong Kong
 
dc.relation.ispartofJoint Annual Scientific Meeting of Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong 2012 Program book
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.titleProtective effects of lycium barbarum polysaccharides on cerebral edema and blood-brain barrier disruption after ischemic stroke
 
dc.typeConference_Paper
 
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<item><contributor.author>Yang, FD</contributor.author>
<contributor.author>Li, SY</contributor.author>
<contributor.author>Yeung, CM</contributor.author>
<contributor.author>Chang, RCC</contributor.author>
<contributor.author>So, KF</contributor.author>
<contributor.author>Wong, D</contributor.author>
<contributor.author>Lo, ACY</contributor.author>
<date.accessioned>2012-08-16T06:04:10Z</date.accessioned>
<date.available>2012-08-16T06:04:10Z</date.available>
<date.issued>2012</date.issued>
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<description>Young Investigators Symposium I (Y3) - Di Yang</description>
<description.abstract>BACKGROUND: Ischemic stroke is a destructive cerebrovascular disease and one of the leading causes of death worldwide. The long term disability after stroke induces heavy burden both to the patients and the society. Yet, no effective neuroprotective agents are available. The polysaccharides extracted from the fruits of wolfberry, Lycium barbarum (LBP), showed neuroprotective and immune-modulative functions. We aim to evaluate the protective effects of LBP in experimental stroke using a focal cerebral ischemia/reperfusion (I/R) model. METHODS: C57BL/6N mice were subjected to 2 h of middle cerebral artery occlusion (MCAO) followed by 22 h of reperfusion. Prior to ischemia induction, animals were treated with either vehicle (PBS) or LBP daily for 7 days. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement and immunohistochemical analysis as well as Western blot experiments. Evans blue (EB) extravasation experiment was performed to determine blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP treatment significantly improved neurological scores and decreased infarct size, hemispheric swelling and water content as well as reduced EB extravasation. In addition, fewer apoptotic cells were identified in the LBP-treated brains by TUNEL assay. Immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were also significantly decreased in LBP-treated brains. We further observed a reduction of nuclear factor-&#954;B translocation and I&#954;B expression after LBP treatment. CONCLUSION: Seven-day LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin water channel up-regulation and glial activation. The protective effects of LBP might partially act through its anti-inflammatory effects. The present study suggests that LBP may be used as a preventive neuroprotectant for ischemic stroke.</description.abstract>
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