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Article: Genome-wide survey of recurrent HBV integration in hepatocellular carcinoma

TitleGenome-wide survey of recurrent HBV integration in hepatocellular carcinoma
Authors
Issue Date2012
PublisherNature Publishing Group. The Journal's web site is located at http://www.genetics.nature.com
Citation
Nature Genetics, 2012, v. 44 n. 7, p. 765-769 How to Cite?
Abstract
To survey hepatitis B virus (HBV) integration in liver cancer genomes, we conducted massively parallel sequencing of 81 HBV-positive and 7 HBV-negative hepatocellular carcinomas (HCCs) and adjacent normal tissues. We found that HBV integration is observed more frequently in the tumors (86.4%) than in adjacent liver tissues (30.7%). Copy-number variations (CNVs) were significantly increased at HBV breakpoint locations where chromosomal instability was likely induced. Approximately 40% of HBV breakpoints within the HBV genome were located within a 1,800-bp region where the viral enhancer, X gene and core gene are located. We also identified recurrent HBV integration events (in ≥4 HCCs) that were validated by RNA sequencing (RNA-seq) and Sanger sequencing at the known and putative cancer-related TERT, MLL4 and CCNE1 genes, which showed upregulated gene expression in tumor versus normal tissue. We also report evidence that suggests that the number of HBV integrations is associated with patient survival. © 2012 Nature America, Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/159928
ISSN
2013 Impact Factor: 29.648
2013 SCImago Journal Rankings: 24.052
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSung, WKen_HK
dc.contributor.authorZheng, Hen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorChen, Ren_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorLee, NPen_HK
dc.contributor.authorLee, WHen_HK
dc.contributor.authorAriyaratne, PNen_HK
dc.contributor.authorTennakoon, Cen_HK
dc.contributor.authorMulawadi, FHen_HK
dc.contributor.authorWong, KFen_HK
dc.contributor.authorLiu, AMen_HK
dc.contributor.authorPoon, RTen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorChan, KLen_HK
dc.contributor.authorGong, Zen_HK
dc.contributor.authorHu, Yen_HK
dc.contributor.authorLin, Zen_HK
dc.contributor.authorWang, Gen_HK
dc.contributor.authorZhang, Qen_HK
dc.contributor.authorBarber, TDen_HK
dc.contributor.authorChou, WCen_HK
dc.contributor.authorAggarwal, Aen_HK
dc.contributor.authorHao, Ken_HK
dc.contributor.authorZhou, Wen_HK
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorHardwick, Jen_HK
dc.contributor.authorBuser, Cen_HK
dc.contributor.authorXu, Jen_HK
dc.contributor.authorKan, Zen_HK
dc.contributor.authorDai, Hen_HK
dc.contributor.authorMao, Men_HK
dc.contributor.authorReinhard, Cen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorLuk, JMen_HK
dc.date.accessioned2012-08-16T05:59:36Z-
dc.date.available2012-08-16T05:59:36Z-
dc.date.issued2012en_HK
dc.identifier.citationNature Genetics, 2012, v. 44 n. 7, p. 765-769en_HK
dc.identifier.issn1061-4036en_HK
dc.identifier.urihttp://hdl.handle.net/10722/159928-
dc.description.abstractTo survey hepatitis B virus (HBV) integration in liver cancer genomes, we conducted massively parallel sequencing of 81 HBV-positive and 7 HBV-negative hepatocellular carcinomas (HCCs) and adjacent normal tissues. We found that HBV integration is observed more frequently in the tumors (86.4%) than in adjacent liver tissues (30.7%). Copy-number variations (CNVs) were significantly increased at HBV breakpoint locations where chromosomal instability was likely induced. Approximately 40% of HBV breakpoints within the HBV genome were located within a 1,800-bp region where the viral enhancer, X gene and core gene are located. We also identified recurrent HBV integration events (in ≥4 HCCs) that were validated by RNA sequencing (RNA-seq) and Sanger sequencing at the known and putative cancer-related TERT, MLL4 and CCNE1 genes, which showed upregulated gene expression in tumor versus normal tissue. We also report evidence that suggests that the number of HBV integrations is associated with patient survival. © 2012 Nature America, Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.genetics.nature.comen_HK
dc.relation.ispartofNature Geneticsen_HK
dc.titleGenome-wide survey of recurrent HBV integration in hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailPoon, RT: poontp@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailReinhard, C: reinhard_christoph@lilly.com-
dc.identifier.emailWang, J: wangj@genomics.org.cn-
dc.identifier.authorityPoon, RT=rp00446en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/ng.2295en_HK
dc.identifier.pmid22634754-
dc.identifier.scopuseid_2-s2.0-84862999344en_HK
dc.identifier.hkuros202939en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862999344&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue7en_HK
dc.identifier.spage765en_HK
dc.identifier.epage769en_HK
dc.identifier.eissn1546-1718-
dc.identifier.isiWOS:000305886900009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSung, WK=13310059700en_HK
dc.identifier.scopusauthoridZheng, H=25637991300en_HK
dc.identifier.scopusauthoridLi, S=55054179100en_HK
dc.identifier.scopusauthoridChen, R=16205085300en_HK
dc.identifier.scopusauthoridLiu, X=36480200200en_HK
dc.identifier.scopusauthoridLi, Y=23766780600en_HK
dc.identifier.scopusauthoridLee, NP=55266265700en_HK
dc.identifier.scopusauthoridLee, WH=55266206700en_HK
dc.identifier.scopusauthoridAriyaratne, PN=14619096800en_HK
dc.identifier.scopusauthoridTennakoon, C=36100598900en_HK
dc.identifier.scopusauthoridMulawadi, FH=36100474400en_HK
dc.identifier.scopusauthoridWong, KF=49362744900en_HK
dc.identifier.scopusauthoridLiu, AM=36134439500en_HK
dc.identifier.scopusauthoridPoon, RT=7103097223en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridChan, KL=55266003200en_HK
dc.identifier.scopusauthoridGong, Z=55063247600en_HK
dc.identifier.scopusauthoridHu, Y=10141048500en_HK
dc.identifier.scopusauthoridLin, Z=55224461700en_HK
dc.identifier.scopusauthoridWang, G=55224881200en_HK
dc.identifier.scopusauthoridZhang, Q=37002296000en_HK
dc.identifier.scopusauthoridBarber, TD=7102704248en_HK
dc.identifier.scopusauthoridChou, WC=55224836100en_HK
dc.identifier.scopusauthoridAggarwal, A=55224844600en_HK
dc.identifier.scopusauthoridHao, K=55224512300en_HK
dc.identifier.scopusauthoridZhou, W=55224747200en_HK
dc.identifier.scopusauthoridZhang, C=9747304800en_HK
dc.identifier.scopusauthoridHardwick, J=7101940088en_HK
dc.identifier.scopusauthoridBuser, C=7004680051en_HK
dc.identifier.scopusauthoridXu, J=54409688500en_HK
dc.identifier.scopusauthoridKan, Z=55224518000en_HK
dc.identifier.scopusauthoridDai, H=55224644500en_HK
dc.identifier.scopusauthoridMao, M=55224776400en_HK
dc.identifier.scopusauthoridReinhard, C=55224540400en_HK
dc.identifier.scopusauthoridWang, J=36984389800en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.citeulike10717104-

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