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Article: Identification and characterization of tropomyosin 3 associated with granulin-epithelin precursor in human hepatocellular carcinoma

TitleIdentification and characterization of tropomyosin 3 associated with granulin-epithelin precursor in human hepatocellular carcinoma
Authors
KeywordsCancer cell
Cancer survival
Case report
Cytoplasm
Immunohistochemistry
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2012, v. 7 n. 7 How to Cite?
AbstractBackground and Aim: Granulin-epithelin precursor (GEP) has previously been reported to control cancer growth, invasion, chemo-resistance, and served as novel therapeutic target for cancer treatment. However, the nature and characteristics of GEP interacting partner remain unclear. The present study aims to identify and characterize the novel predominant interacting partner of GEP using co-immunoprecipitation and mass spectrometry. Methods and Results: Specific anti-GEP monoclonal antibody was used to capture GEP and its interacting partner from the protein extract of the liver cancer cells Hep3B. The precipitated proteins were analyzed by SDS-PAGE, followed by mass spectrometry and the protein identity was demonstrated to be tropomyosin 3 (TPM3). The interaction has been validated in additional cell models using anti-TPM3 antibody and immunoblot to confirm GEP as the interacting partner. GEP and TPM3 expressions were then examined by real-time quantitative RT-PCR in clinical samples, and their transcript levels were significantly correlated. Elevated TPM3 levels were observed in liver cancer compared with the adjacent non-tumorous liver, and patients with elevated TPM3 levels were shown to have poor recurrence-free survival. Protein expression of GEP and TPM3 was observed only in the cytoplasm of liver cancer cells by immunohistochemical staining. Conclusions: TPM3 is an interacting partner of GEP and may play an important role in hepatocarcinogenesis. © 2012 Lam et al.
Persistent Identifierhttp://hdl.handle.net/10722/159921
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, CYen_HK
dc.contributor.authorYip, CWen_HK
dc.contributor.authorPoon, TCWen_HK
dc.contributor.authorCheng, CKCen_HK
dc.contributor.authorNg, EWYen_HK
dc.contributor.authorWong, NCLen_HK
dc.contributor.authorCheung, PFYen_HK
dc.contributor.authorLai, PBSen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorCheung, STen_HK
dc.date.accessioned2012-08-16T05:59:32Z-
dc.date.available2012-08-16T05:59:32Z-
dc.date.issued2012en_HK
dc.identifier.citationPlos One, 2012, v. 7 n. 7en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/159921-
dc.description.abstractBackground and Aim: Granulin-epithelin precursor (GEP) has previously been reported to control cancer growth, invasion, chemo-resistance, and served as novel therapeutic target for cancer treatment. However, the nature and characteristics of GEP interacting partner remain unclear. The present study aims to identify and characterize the novel predominant interacting partner of GEP using co-immunoprecipitation and mass spectrometry. Methods and Results: Specific anti-GEP monoclonal antibody was used to capture GEP and its interacting partner from the protein extract of the liver cancer cells Hep3B. The precipitated proteins were analyzed by SDS-PAGE, followed by mass spectrometry and the protein identity was demonstrated to be tropomyosin 3 (TPM3). The interaction has been validated in additional cell models using anti-TPM3 antibody and immunoblot to confirm GEP as the interacting partner. GEP and TPM3 expressions were then examined by real-time quantitative RT-PCR in clinical samples, and their transcript levels were significantly correlated. Elevated TPM3 levels were observed in liver cancer compared with the adjacent non-tumorous liver, and patients with elevated TPM3 levels were shown to have poor recurrence-free survival. Protein expression of GEP and TPM3 was observed only in the cytoplasm of liver cancer cells by immunohistochemical staining. Conclusions: TPM3 is an interacting partner of GEP and may play an important role in hepatocarcinogenesis. © 2012 Lam et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCancer cell-
dc.subjectCancer survival-
dc.subjectCase report-
dc.subjectCytoplasm-
dc.subjectImmunohistochemistry-
dc.titleIdentification and characterization of tropomyosin 3 associated with granulin-epithelin precursor in human hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailCheung, ST: stcheung@hkucc.hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityCheung, ST=rp00457en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0040324en_HK
dc.identifier.pmid22792281-
dc.identifier.pmcidPMC3391266-
dc.identifier.scopuseid_2-s2.0-84863646269en_HK
dc.identifier.hkuros202757en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863646269&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue7en_HK
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000306461800070-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLam, CY=55307554900en_HK
dc.identifier.scopusauthoridYip, CW=54685625700en_HK
dc.identifier.scopusauthoridPoon, TCW=55309022000en_HK
dc.identifier.scopusauthoridCheng, CKC=37030630100en_HK
dc.identifier.scopusauthoridNg, EWY=55308427200en_HK
dc.identifier.scopusauthoridWong, NCL=37032421100en_HK
dc.identifier.scopusauthoridCheung, PFY=37030665700en_HK
dc.identifier.scopusauthoridLai, PBS=55277763600en_HK
dc.identifier.scopusauthoridNg, IOL=55261303200en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridCheung, ST=7202473497en_HK

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