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Article: Metabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae

TitleMetabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae
Authors
KeywordsCalycosin
Conjugation
Isoflavone
Metabolism
Zebrafish larvae
Issue Date2012
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00498254.asp
Citation
Xenobiotica, 2012, v. 42 n. 3, p. 294-303 How to Cite?
AbstractAlthough zebrafish has become a popular animal model for drug discovery and screening, drug metabolism in zebrafish remains largely unknown. In this study, we probed the metabolic capability of zebrafish larvae with calycosin, one of the major isoflavone constituents of Radix Astragali that was previously demonstrated to be angiogenic in the zebrafish model. The metabolism of calycosin and accumulation of its metabolites in zebrafish larvae were determined using an LCMS/MS method. Calycosin showed a slow but steady decrease from the culture medium as well as a steady accumulation in zebrafish larvae. Calycosin underwent major conjugation and minor oxidation in zebrafish larvae. A total of ten calycosin metabolites formed from glucuronidation, glucosylation, sulfation, oxidation or a combination of two of these metabolisms were identified, most of which were reported for the first time. Most metabolites increased steadily in the larvae over 24-h experimental period. The dominant phase II conjugation of calycosin in zebrafish larvae matched well with existing knowledge of isoflavone metabolism in mammalians. The findings shed a light in certain degree of similarity of phase II drug metabolism between zebrafish larvae and mammals and warrant further investigation on feasibility of adopting the zebrafish larvae as a whole-organism model for examining drug metabolism. © 2012 Informa UK, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/159770
ISSN
2014 Impact Factor: 2.199
2014 SCImago Journal Rankings: 0.617
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, Gen_HK
dc.contributor.authorSiu, SOen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorChu, IKen_HK
dc.contributor.authorKwan, YWen_HK
dc.contributor.authorChan, SWen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorYan, Ren_HK
dc.contributor.authorLee, SMYen_HK
dc.date.accessioned2012-08-16T05:56:14Z-
dc.date.available2012-08-16T05:56:14Z-
dc.date.issued2012en_HK
dc.identifier.citationXenobiotica, 2012, v. 42 n. 3, p. 294-303en_HK
dc.identifier.issn0049-8254en_HK
dc.identifier.urihttp://hdl.handle.net/10722/159770-
dc.description.abstractAlthough zebrafish has become a popular animal model for drug discovery and screening, drug metabolism in zebrafish remains largely unknown. In this study, we probed the metabolic capability of zebrafish larvae with calycosin, one of the major isoflavone constituents of Radix Astragali that was previously demonstrated to be angiogenic in the zebrafish model. The metabolism of calycosin and accumulation of its metabolites in zebrafish larvae were determined using an LCMS/MS method. Calycosin showed a slow but steady decrease from the culture medium as well as a steady accumulation in zebrafish larvae. Calycosin underwent major conjugation and minor oxidation in zebrafish larvae. A total of ten calycosin metabolites formed from glucuronidation, glucosylation, sulfation, oxidation or a combination of two of these metabolisms were identified, most of which were reported for the first time. Most metabolites increased steadily in the larvae over 24-h experimental period. The dominant phase II conjugation of calycosin in zebrafish larvae matched well with existing knowledge of isoflavone metabolism in mammalians. The findings shed a light in certain degree of similarity of phase II drug metabolism between zebrafish larvae and mammals and warrant further investigation on feasibility of adopting the zebrafish larvae as a whole-organism model for examining drug metabolism. © 2012 Informa UK, Ltd.en_HK
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00498254.aspen_HK
dc.relation.ispartofXenobioticaen_HK
dc.subjectCalycosinen_HK
dc.subjectConjugationen_HK
dc.subjectIsoflavoneen_HK
dc.subjectMetabolismen_HK
dc.subjectZebrafish larvaeen_HK
dc.titleMetabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvaeen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3109/00498254.2011.617015en_HK
dc.identifier.pmid21961561-
dc.identifier.scopuseid_2-s2.0-84863061192en_HK
dc.identifier.hkuros203462en_US
dc.identifier.hkuros204648-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863061192&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue3en_HK
dc.identifier.spage294en_HK
dc.identifier.epage303en_HK
dc.identifier.isiWOS:000299696900007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHu, G=55267315700en_HK
dc.identifier.scopusauthoridSiu, SO=55267757800en_HK
dc.identifier.scopusauthoridLi, S=36627361600en_HK
dc.identifier.scopusauthoridChu, IK=55267215600en_HK
dc.identifier.scopusauthoridKwan, YW=7005662153en_HK
dc.identifier.scopusauthoridChan, SW=55346861600en_HK
dc.identifier.scopusauthoridLeung, GPH=35963668200en_HK
dc.identifier.scopusauthoridYan, R=36024582100en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK

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