Article: Metabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae

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TitleMetabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae
AuthorsHu, G2
Siu, SO1 2
Li, S2
Chu, IK1
Kwan, YW4
Chan, SW3
Leung, GPH1
Yan, R2
Lee, SMY2
KeywordsCalycosin
Conjugation
Isoflavone
Metabolism
Zebrafish larvae
Issue Date2012
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00498254.asp
CitationXenobiotica, 2012, v. 42 n. 3, p. 294-303 [How to Cite?]
DOI: http://dx.doi.org/10.3109/00498254.2011.617015
AbstractAlthough zebrafish has become a popular animal model for drug discovery and screening, drug metabolism in zebrafish remains largely unknown. In this study, we probed the metabolic capability of zebrafish larvae with calycosin, one of the major isoflavone constituents of Radix Astragali that was previously demonstrated to be angiogenic in the zebrafish model. The metabolism of calycosin and accumulation of its metabolites in zebrafish larvae were determined using an LCMS/MS method. Calycosin showed a slow but steady decrease from the culture medium as well as a steady accumulation in zebrafish larvae. Calycosin underwent major conjugation and minor oxidation in zebrafish larvae. A total of ten calycosin metabolites formed from glucuronidation, glucosylation, sulfation, oxidation or a combination of two of these metabolisms were identified, most of which were reported for the first time. Most metabolites increased steadily in the larvae over 24-h experimental period. The dominant phase II conjugation of calycosin in zebrafish larvae matched well with existing knowledge of isoflavone metabolism in mammalians. The findings shed a light in certain degree of similarity of phase II drug metabolism between zebrafish larvae and mammals and warrant further investigation on feasibility of adopting the zebrafish larvae as a whole-organism model for examining drug metabolism. © 2012 Informa UK, Ltd.
ISSN0049-8254
2011 Impact Factor: 1.791
2011 SCImago Journal Rankings: 0.191
DOIhttp://dx.doi.org/10.3109/00498254.2011.617015
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHu, G
dc.contributor.authorSiu, SO
dc.contributor.authorLi, S
dc.contributor.authorChu, IK
dc.contributor.authorKwan, YW
dc.contributor.authorChan, SW
dc.contributor.authorLeung, GPH
dc.contributor.authorYan, R
dc.contributor.authorLee, SMY
dc.date.accessioned2012-08-16T05:56:14Z
dc.date.available2012-08-16T05:56:14Z
dc.date.issued2012
dc.description.abstractAlthough zebrafish has become a popular animal model for drug discovery and screening, drug metabolism in zebrafish remains largely unknown. In this study, we probed the metabolic capability of zebrafish larvae with calycosin, one of the major isoflavone constituents of Radix Astragali that was previously demonstrated to be angiogenic in the zebrafish model. The metabolism of calycosin and accumulation of its metabolites in zebrafish larvae were determined using an LCMS/MS method. Calycosin showed a slow but steady decrease from the culture medium as well as a steady accumulation in zebrafish larvae. Calycosin underwent major conjugation and minor oxidation in zebrafish larvae. A total of ten calycosin metabolites formed from glucuronidation, glucosylation, sulfation, oxidation or a combination of two of these metabolisms were identified, most of which were reported for the first time. Most metabolites increased steadily in the larvae over 24-h experimental period. The dominant phase II conjugation of calycosin in zebrafish larvae matched well with existing knowledge of isoflavone metabolism in mammalians. The findings shed a light in certain degree of similarity of phase II drug metabolism between zebrafish larvae and mammals and warrant further investigation on feasibility of adopting the zebrafish larvae as a whole-organism model for examining drug metabolism. © 2012 Informa UK, Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationXenobiotica, 2012, v. 42 n. 3, p. 294-303 [How to Cite?]
DOI: http://dx.doi.org/10.3109/00498254.2011.617015
dc.identifier.doihttp://dx.doi.org/10.3109/00498254.2011.617015
dc.identifier.epage303
dc.identifier.hkuros203462
dc.identifier.hkuros204648
dc.identifier.isiWOS:000299696900007
dc.identifier.issn0049-8254
2011 Impact Factor: 1.791
2011 SCImago Journal Rankings: 0.191
dc.identifier.issue3
dc.identifier.pmid21961561
dc.identifier.scopuseid_2-s2.0-84863061192
dc.identifier.spage294
dc.identifier.urihttp://hdl.handle.net/10722/159770
dc.identifier.volume42
dc.languageeng
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00498254.asp
dc.publisher.placeUnited Kingdom
dc.relation.ispartofXenobiotica
dc.relation.referencesReferences in Scopus
dc.subjectCalycosin
dc.subjectConjugation
dc.subjectIsoflavone
dc.subjectMetabolism
dc.subjectZebrafish larvae
dc.titleMetabolism of calycosin, an isoflavone from Astragali Radix, in zebrafish larvae
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. University of Macau
  3. Hong Kong Polytechnic University
  4. Chinese University of Hong Kong