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Article: Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo

TitlePro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo
Authors
Issue Date2012
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
Citation
Molecular Medicine Reports, 2012, v. 5 n. 3, p. 805-811 How to Cite?
Abstract
Astragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (HUVECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expression of vascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase--domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1/VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both VEGF- and Akt-dependent signaling pathways.
DescriptionThe article can be viewed at http://www.spandidos-publications.com/10.3892/mmr.2011.716
Persistent Identifierhttp://hdl.handle.net/10722/159769
ISSN
2013 Impact Factor: 1.484
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. University of Macau
  3. Hong Kong Polytechnic University
  4. Chinese University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorHu, Gen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorLi, ZHen_HK
dc.contributor.authorLam, COen_HK
dc.contributor.authorHong, SJen_HK
dc.contributor.authorKwan, YWen_HK
dc.contributor.authorChan, SWen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorLee, SMYen_HK
dc.date.accessioned2012-08-16T05:56:14Z-
dc.date.available2012-08-16T05:56:14Z-
dc.date.issued2012en_HK
dc.identifier.citationMolecular Medicine Reports, 2012, v. 5 n. 3, p. 805-811en_HK
dc.identifier.issn1791-2997en_HK
dc.identifier.urihttp://hdl.handle.net/10722/159769-
dc.descriptionThe article can be viewed at http://www.spandidos-publications.com/10.3892/mmr.2011.716-
dc.description.abstractAstragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (HUVECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expression of vascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase--domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1/VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both VEGF- and Akt-dependent signaling pathways.en_HK
dc.languageengen_US
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/-
dc.relation.ispartofMolecular Medicine Reportsen_HK
dc.subject.meshAngiogenesis Inducing Agents - pharmacologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAstragalus Plant - chemistryen_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDrugs, Chinese Herbal - chemistryen_HK
dc.subject.meshGene Expression Regulation - drug effectsen_HK
dc.subject.meshHuman Umbilical Vein Endothelial Cells - drug effects - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIndoles - pharmacologyen_HK
dc.subject.meshNeovascularization, Physiologic - drug effectsen_HK
dc.subject.meshPhosphatidylinositols - pharmacologyen_HK
dc.subject.meshProto-Oncogene Proteins c-akt - antagonists & inhibitors - genetics - metabolismen_HK
dc.subject.meshPyrroles - pharmacologyen_HK
dc.subject.meshReceptors, Vascular Endothelial Growth Factor - genetics - metabolismen_HK
dc.subject.meshSaponins - pharmacologyen_HK
dc.subject.meshTriterpenes - pharmacologyen_HK
dc.subject.meshVascular Endothelial Growth Factor A - genetics - metabolismen_HK
dc.subject.meshVascular Endothelial Growth Factor Receptor-2 - genetics - metabolismen_HK
dc.subject.meshZebrafishen_HK
dc.titlePro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivoen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3892/mmr.2011.716en_HK
dc.identifier.pmid22179585en_HK
dc.identifier.scopuseid_2-s2.0-84863042713en_HK
dc.identifier.hkuros203461en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863042713&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue3en_HK
dc.identifier.spage805en_HK
dc.identifier.epage811en_HK
dc.identifier.isiWOS:000300272400038-
dc.identifier.scopusauthoridZhang, Y=55268958700en_HK
dc.identifier.scopusauthoridHu, G=55267312800en_HK
dc.identifier.scopusauthoridLi, S=36627361600en_HK
dc.identifier.scopusauthoridLi, ZH=8439008600en_HK
dc.identifier.scopusauthoridLam, CO=55267201400en_HK
dc.identifier.scopusauthoridHong, SJ=23396465100en_HK
dc.identifier.scopusauthoridKwan, YW=7005662153en_HK
dc.identifier.scopusauthoridChan, SW=7404255670en_HK
dc.identifier.scopusauthoridLeung, GPH=35963668200en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK

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