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Article: Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo

TitlePro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo
Authors
Issue Date2012
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
Citation
Molecular Medicine Reports, 2012, v. 5 n. 3, p. 805-811 How to Cite?
Abstract
Astragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (HUVECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expression of vascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase--domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1/VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both VEGF- and Akt-dependent signaling pathways.
DescriptionThe article can be viewed at http://www.spandidos-publications.com/10.3892/mmr.2011.716
Persistent Identifierhttp://hdl.handle.net/10722/159769
ISSN
2013 Impact Factor: 1.484
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorHu, Gen_HK
dc.contributor.authorLi, Sen_HK
dc.contributor.authorLi, ZHen_HK
dc.contributor.authorLam, COen_HK
dc.contributor.authorHong, SJen_HK
dc.contributor.authorKwan, YWen_HK
dc.contributor.authorChan, SWen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorLee, SMYen_HK
dc.date.accessioned2012-08-16T05:56:14Z-
dc.date.available2012-08-16T05:56:14Z-
dc.date.issued2012en_HK
dc.identifier.citationMolecular Medicine Reports, 2012, v. 5 n. 3, p. 805-811en_HK
dc.identifier.issn1791-2997en_HK
dc.identifier.urihttp://hdl.handle.net/10722/159769-
dc.descriptionThe article can be viewed at http://www.spandidos-publications.com/10.3892/mmr.2011.716-
dc.description.abstractAstragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (HUVECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expression of vascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase--domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1/VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both VEGF- and Akt-dependent signaling pathways.en_HK
dc.languageengen_US
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/-
dc.relation.ispartofMolecular Medicine Reportsen_HK
dc.subject.meshAngiogenesis Inducing Agents - pharmacologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAstragalus Plant - chemistryen_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDrugs, Chinese Herbal - chemistryen_HK
dc.subject.meshGene Expression Regulation - drug effectsen_HK
dc.subject.meshHuman Umbilical Vein Endothelial Cells - drug effects - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIndoles - pharmacologyen_HK
dc.subject.meshNeovascularization, Physiologic - drug effectsen_HK
dc.subject.meshPhosphatidylinositols - pharmacologyen_HK
dc.subject.meshProto-Oncogene Proteins c-akt - antagonists & inhibitors - genetics - metabolismen_HK
dc.subject.meshPyrroles - pharmacologyen_HK
dc.subject.meshReceptors, Vascular Endothelial Growth Factor - genetics - metabolismen_HK
dc.subject.meshSaponins - pharmacologyen_HK
dc.subject.meshTriterpenes - pharmacologyen_HK
dc.subject.meshVascular Endothelial Growth Factor A - genetics - metabolismen_HK
dc.subject.meshVascular Endothelial Growth Factor Receptor-2 - genetics - metabolismen_HK
dc.subject.meshZebrafishen_HK
dc.titlePro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivoen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3892/mmr.2011.716en_HK
dc.identifier.pmid22179585en_HK
dc.identifier.scopuseid_2-s2.0-84863042713en_HK
dc.identifier.hkuros203461en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863042713&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue3en_HK
dc.identifier.spage805en_HK
dc.identifier.epage811en_HK
dc.identifier.isiWOS:000300272400038-
dc.identifier.scopusauthoridZhang, Y=55268958700en_HK
dc.identifier.scopusauthoridHu, G=55267312800en_HK
dc.identifier.scopusauthoridLi, S=36627361600en_HK
dc.identifier.scopusauthoridLi, ZH=8439008600en_HK
dc.identifier.scopusauthoridLam, CO=55267201400en_HK
dc.identifier.scopusauthoridHong, SJ=23396465100en_HK
dc.identifier.scopusauthoridKwan, YW=7005662153en_HK
dc.identifier.scopusauthoridChan, SW=7404255670en_HK
dc.identifier.scopusauthoridLeung, GPH=35963668200en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK

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