Article: Pro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo

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TitlePro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo
AuthorsZhang, Y2
Hu, G2
Li, S2
Li, ZH2
Lam, CO2
Hong, SJ2
Kwan, YW4
Chan, SW3
Leung, GPH1
Lee, SMY2
Issue Date2012
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
CitationMolecular Medicine Reports, 2012, v. 5 n. 3, p. 805-811 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2011.716
AbstractAstragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (HUVECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expression of vascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase--domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1/VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both VEGF- and Akt-dependent signaling pathways.
DescriptionThe article can be viewed at http://www.spandidos-publications.com/10.3892/mmr.2011.716
ISSN1791-2997
2011 Impact Factor: 0.418
2011 SCImago Journal Rankings: 0.041
DOIhttp://dx.doi.org/10.3892/mmr.2011.716
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhang, Y
dc.contributor.authorHu, G
dc.contributor.authorLi, S
dc.contributor.authorLi, ZH
dc.contributor.authorLam, CO
dc.contributor.authorHong, SJ
dc.contributor.authorKwan, YW
dc.contributor.authorChan, SW
dc.contributor.authorLeung, GPH
dc.contributor.authorLee, SMY
dc.date.accessioned2012-08-16T05:56:14Z
dc.date.available2012-08-16T05:56:14Z
dc.date.issued2012
dc.description.abstractAstragaloside IV (AS-IV) is a natural product isolated from the Chinese medical herb, Radix Astragali, which has been reported to be a potential candidate for treating diseases associated with abnormal angiogenesis; however, the effect of AS-IV on angiogenesis and its underlying mechanisms are yet to be fully elucidated. In the present study, we investigated the angiogenic effect of AS-IV in vitro using human umbilical vein endothelial cells (HUVECs), and in vivo using zebrafish. AS-IV was found to stimulate the proliferation and migration of HUVECs in an XTT assay and a wound healing migration assay, respectively. Moreover, AS-IV stimulated the invasive ability of HUVECs and significantly increased the mean tube length of HUVECs in Matrigel. AS-IV induced an angiogenic response in HUVECs and enhanced mRNA expression of vascular endothelial growth factor (VEGF) and a VEGF receptor known as kinase--domain region/fetal liver kinase-1/VEGF receptor 2 (KDR/Flk-1/VEGFR2), as well as activation of Akt as demonstrated by quantitative real-time PCR and Western blot analysis, respectively. The AS-IV-induced proliferation of HUVECs was capable of being suppressed by a KDR inhibitor (SU5416) and an Akt inhibitor (SH-6). AS-IV also rescued blood vessel loss in Tg (fli-1:EGFP) zebrafish. Altogether, our results suggest that AS-IV exerts potential pro-angiogenic effects in vitro and in vivo, and that its pro-angiogenic activity probably involves both VEGF- and Akt-dependent signaling pathways.
dc.description.natureLink_to_subscribed_fulltext
dc.descriptionThe article can be viewed at http://www.spandidos-publications.com/10.3892/mmr.2011.716
dc.identifier.citationMolecular Medicine Reports, 2012, v. 5 n. 3, p. 805-811 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2011.716
dc.identifier.doihttp://dx.doi.org/10.3892/mmr.2011.716
dc.identifier.epage811
dc.identifier.hkuros203461
dc.identifier.issn1791-2997
2011 Impact Factor: 0.418
2011 SCImago Journal Rankings: 0.041
dc.identifier.issue3
dc.identifier.pmid22179585
dc.identifier.scopuseid_2-s2.0-84863042713
dc.identifier.spage805
dc.identifier.urihttp://hdl.handle.net/10722/159769
dc.identifier.volume5
dc.languageeng
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
dc.relation.ispartofMolecular Medicine Reports
dc.relation.referencesReferences in Scopus
dc.subject.meshAngiogenesis Inducing Agents - pharmacology
dc.subject.meshAnimals
dc.subject.meshAstragalus Plant - chemistry
dc.subject.meshCell Movement
dc.subject.meshCell Proliferation - drug effects
dc.subject.meshCells, Cultured
dc.subject.meshDrugs, Chinese Herbal - chemistry
dc.subject.meshGene Expression Regulation - drug effects
dc.subject.meshHuman Umbilical Vein Endothelial Cells - drug effects - metabolism
dc.subject.meshHumans
dc.subject.meshIndoles - pharmacology
dc.subject.meshNeovascularization, Physiologic - drug effects
dc.subject.meshPhosphatidylinositols - pharmacology
dc.subject.meshProto-Oncogene Proteins c-akt - antagonists & inhibitors - genetics - metabolism
dc.subject.meshPyrroles - pharmacology
dc.subject.meshReceptors, Vascular Endothelial Growth Factor - genetics - metabolism
dc.subject.meshSaponins - pharmacology
dc.subject.meshTriterpenes - pharmacology
dc.subject.meshVascular Endothelial Growth Factor A - genetics - metabolism
dc.subject.meshVascular Endothelial Growth Factor Receptor-2 - genetics - metabolism
dc.subject.meshZebrafish
dc.titlePro-angiogenic activity of astragaloside IV in HUVECs in vitro and zebrafish in vivo
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. University of Macau
  3. Hong Kong Polytechnic University
  4. Chinese University of Hong Kong