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Article: Transcriptional profiling of angiogenesis activities of calycosin in zebrafish

TitleTranscriptional profiling of angiogenesis activities of calycosin in zebrafish
Authors
Issue Date2011
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htm
Citation
Molecular Biosystems, 2011, v. 7 n. 11, p. 3112-3121 How to Cite?
AbstractAngiogenesis plays an important role in a wide range of physiological processes and many diseases are associated with the dysregulation of angiogenesis. The commonly used Chinese herbal medicine Radix Astragali (known as Huang qi in Chinese) is a potential candidate for treating this type of disease. Calycosin, a major isoflavonoid in Radix Astragali, was identified in our earlier study and shown to induce angiogenesis in human umbilical vein endothelial cells (HUVEC) in vitro and in zebrafish embryos in vivo. Using zebrafish as a testing model, we investigated the angiogenic effect of calycosin on the subintestinal vessels (SIVs) in zebrafish embryos. Our findings using transcriptional profiling by deep sequencing, and confirmed by quantitative real-time PCR (qPCR), demonstrate that calycosin modulated vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and ErbB signaling pathways. The inhibitory effects of calycosin-induced phenotypic responses by several pathway-specific inhibitors (VRI, SU5402, MEK1/2 Inhibitor, Wortmannin and LY294002) further identified the potential involvement of VEGF(R) and FGF(R) signaling pathways in the angiogenic activities of calycosin. We present a comprehensive framework of study using fluorescence microscopy, transcriptomics and qPCR to demonstrate the proangiogenic effects of calycosin in vivo. The data have elucidated the connection between morphological observations and genomic evidence, indicating the potential roles of several key signaling pathways in angiogenesis. This journal is © The Royal Society of Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/159767
ISSN
2014 Impact Factor: 3.210
2014 SCImago Journal Rankings: 1.205
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Sen_HK
dc.contributor.authorLou, Sen_HK
dc.contributor.authorLei, BUWen_HK
dc.contributor.authorChan, TFen_HK
dc.contributor.authorKwan, YWen_HK
dc.contributor.authorChan, SWen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorTsui, SKWen_HK
dc.contributor.authorLee, SMYen_HK
dc.date.accessioned2012-08-16T05:56:13Z-
dc.date.available2012-08-16T05:56:13Z-
dc.date.issued2011en_HK
dc.identifier.citationMolecular Biosystems, 2011, v. 7 n. 11, p. 3112-3121en_HK
dc.identifier.issn1742-206Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/159767-
dc.description.abstractAngiogenesis plays an important role in a wide range of physiological processes and many diseases are associated with the dysregulation of angiogenesis. The commonly used Chinese herbal medicine Radix Astragali (known as Huang qi in Chinese) is a potential candidate for treating this type of disease. Calycosin, a major isoflavonoid in Radix Astragali, was identified in our earlier study and shown to induce angiogenesis in human umbilical vein endothelial cells (HUVEC) in vitro and in zebrafish embryos in vivo. Using zebrafish as a testing model, we investigated the angiogenic effect of calycosin on the subintestinal vessels (SIVs) in zebrafish embryos. Our findings using transcriptional profiling by deep sequencing, and confirmed by quantitative real-time PCR (qPCR), demonstrate that calycosin modulated vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and ErbB signaling pathways. The inhibitory effects of calycosin-induced phenotypic responses by several pathway-specific inhibitors (VRI, SU5402, MEK1/2 Inhibitor, Wortmannin and LY294002) further identified the potential involvement of VEGF(R) and FGF(R) signaling pathways in the angiogenic activities of calycosin. We present a comprehensive framework of study using fluorescence microscopy, transcriptomics and qPCR to demonstrate the proangiogenic effects of calycosin in vivo. The data have elucidated the connection between morphological observations and genomic evidence, indicating the potential roles of several key signaling pathways in angiogenesis. This journal is © The Royal Society of Chemistry.en_HK
dc.languageengen_US
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htmen_HK
dc.relation.ispartofMolecular BioSystemsen_HK
dc.titleTranscriptional profiling of angiogenesis activities of calycosin in zebrafishen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1039/c1mb05206cen_HK
dc.identifier.pmid21909574-
dc.identifier.scopuseid_2-s2.0-80054026500en_HK
dc.identifier.hkuros203459en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80054026500&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue11en_HK
dc.identifier.spage3112en_HK
dc.identifier.epage3121en_HK
dc.identifier.isiWOS:000295844100020-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, S=36627361600en_HK
dc.identifier.scopusauthoridLou, S=36182669800en_HK
dc.identifier.scopusauthoridLei, BUW=53264097500en_HK
dc.identifier.scopusauthoridChan, TF=7402680855en_HK
dc.identifier.scopusauthoridKwan, YW=7005662153en_HK
dc.identifier.scopusauthoridChan, SW=7404255670en_HK
dc.identifier.scopusauthoridLeung, GPH=35963668200en_HK
dc.identifier.scopusauthoridTsui, SKW=7004961364en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK

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