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Article: Transcriptional profiling of angiogenesis activities of calycosin in zebrafish
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TitleTranscriptional profiling of angiogenesis activities of calycosin in zebrafish
 
AuthorsLi, S2
Lou, S4
Lei, BUW2
Chan, TF4
Kwan, YW4
Chan, SW3
Leung, GPH1
Tsui, SKW4
Lee, SMY2
 
Issue Date2011
 
PublisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htm
 
CitationMolecular Biosystems, 2011, v. 7 n. 11, p. 3112-3121 [How to Cite?]
DOI: http://dx.doi.org/10.1039/c1mb05206c
 
AbstractAngiogenesis plays an important role in a wide range of physiological processes and many diseases are associated with the dysregulation of angiogenesis. The commonly used Chinese herbal medicine Radix Astragali (known as Huang qi in Chinese) is a potential candidate for treating this type of disease. Calycosin, a major isoflavonoid in Radix Astragali, was identified in our earlier study and shown to induce angiogenesis in human umbilical vein endothelial cells (HUVEC) in vitro and in zebrafish embryos in vivo. Using zebrafish as a testing model, we investigated the angiogenic effect of calycosin on the subintestinal vessels (SIVs) in zebrafish embryos. Our findings using transcriptional profiling by deep sequencing, and confirmed by quantitative real-time PCR (qPCR), demonstrate that calycosin modulated vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and ErbB signaling pathways. The inhibitory effects of calycosin-induced phenotypic responses by several pathway-specific inhibitors (VRI, SU5402, MEK1/2 Inhibitor, Wortmannin and LY294002) further identified the potential involvement of VEGF(R) and FGF(R) signaling pathways in the angiogenic activities of calycosin. We present a comprehensive framework of study using fluorescence microscopy, transcriptomics and qPCR to demonstrate the proangiogenic effects of calycosin in vivo. The data have elucidated the connection between morphological observations and genomic evidence, indicating the potential roles of several key signaling pathways in angiogenesis. This journal is © The Royal Society of Chemistry.
 
ISSN1742-206X
2012 Impact Factor: 3.35
2012 SCImago Journal Rankings: 1.388
 
DOIhttp://dx.doi.org/10.1039/c1mb05206c
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLi, S
 
dc.contributor.authorLou, S
 
dc.contributor.authorLei, BUW
 
dc.contributor.authorChan, TF
 
dc.contributor.authorKwan, YW
 
dc.contributor.authorChan, SW
 
dc.contributor.authorLeung, GPH
 
dc.contributor.authorTsui, SKW
 
dc.contributor.authorLee, SMY
 
dc.date.accessioned2012-08-16T05:56:13Z
 
dc.date.available2012-08-16T05:56:13Z
 
dc.date.issued2011
 
dc.description.abstractAngiogenesis plays an important role in a wide range of physiological processes and many diseases are associated with the dysregulation of angiogenesis. The commonly used Chinese herbal medicine Radix Astragali (known as Huang qi in Chinese) is a potential candidate for treating this type of disease. Calycosin, a major isoflavonoid in Radix Astragali, was identified in our earlier study and shown to induce angiogenesis in human umbilical vein endothelial cells (HUVEC) in vitro and in zebrafish embryos in vivo. Using zebrafish as a testing model, we investigated the angiogenic effect of calycosin on the subintestinal vessels (SIVs) in zebrafish embryos. Our findings using transcriptional profiling by deep sequencing, and confirmed by quantitative real-time PCR (qPCR), demonstrate that calycosin modulated vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and ErbB signaling pathways. The inhibitory effects of calycosin-induced phenotypic responses by several pathway-specific inhibitors (VRI, SU5402, MEK1/2 Inhibitor, Wortmannin and LY294002) further identified the potential involvement of VEGF(R) and FGF(R) signaling pathways in the angiogenic activities of calycosin. We present a comprehensive framework of study using fluorescence microscopy, transcriptomics and qPCR to demonstrate the proangiogenic effects of calycosin in vivo. The data have elucidated the connection between morphological observations and genomic evidence, indicating the potential roles of several key signaling pathways in angiogenesis. This journal is © The Royal Society of Chemistry.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMolecular Biosystems, 2011, v. 7 n. 11, p. 3112-3121 [How to Cite?]
DOI: http://dx.doi.org/10.1039/c1mb05206c
 
dc.identifier.doihttp://dx.doi.org/10.1039/c1mb05206c
 
dc.identifier.epage3121
 
dc.identifier.hkuros203459
 
dc.identifier.issn1742-206X
2012 Impact Factor: 3.35
2012 SCImago Journal Rankings: 1.388
 
dc.identifier.issue11
 
dc.identifier.pmid21909574
 
dc.identifier.scopuseid_2-s2.0-80054026500
 
dc.identifier.spage3112
 
dc.identifier.urihttp://hdl.handle.net/10722/159767
 
dc.identifier.volume7
 
dc.languageeng
 
dc.publisherRoyal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htm
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofMolecular BioSystems
 
dc.relation.referencesReferences in Scopus
 
dc.titleTranscriptional profiling of angiogenesis activities of calycosin in zebrafish
 
dc.typeArticle
 
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<contributor.author>Lei, BUW</contributor.author>
<contributor.author>Chan, TF</contributor.author>
<contributor.author>Kwan, YW</contributor.author>
<contributor.author>Chan, SW</contributor.author>
<contributor.author>Leung, GPH</contributor.author>
<contributor.author>Tsui, SKW</contributor.author>
<contributor.author>Lee, SMY</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. University of Macau
  3. Hong Kong Polytechnic University
  4. Chinese University of Hong Kong