Article: Transcriptional profiling of angiogenesis activities of calycosin in zebrafish
| Title | Transcriptional profiling of angiogenesis activities of calycosin in zebrafish |
|---|---|
| Authors | Li, S2 Lou, S4 Lei, BUW2 Chan, TF4 Kwan, YW4 Chan, SW3 Leung, GPH1 Tsui, SKW4 Lee, SMY2 |
| Issue Date | 2011 |
| Publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htm |
| Citation | Molecular Biosystems, 2011, v. 7 n. 11, p. 3112-3121 [How to Cite?] DOI: http://dx.doi.org/10.1039/c1mb05206c |
| Abstract | Angiogenesis plays an important role in a wide range of physiological processes and many diseases are associated with the dysregulation of angiogenesis. The commonly used Chinese herbal medicine Radix Astragali (known as Huang qi in Chinese) is a potential candidate for treating this type of disease. Calycosin, a major isoflavonoid in Radix Astragali, was identified in our earlier study and shown to induce angiogenesis in human umbilical vein endothelial cells (HUVEC) in vitro and in zebrafish embryos in vivo. Using zebrafish as a testing model, we investigated the angiogenic effect of calycosin on the subintestinal vessels (SIVs) in zebrafish embryos. Our findings using transcriptional profiling by deep sequencing, and confirmed by quantitative real-time PCR (qPCR), demonstrate that calycosin modulated vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and ErbB signaling pathways. The inhibitory effects of calycosin-induced phenotypic responses by several pathway-specific inhibitors (VRI, SU5402, MEK1/2 Inhibitor, Wortmannin and LY294002) further identified the potential involvement of VEGF(R) and FGF(R) signaling pathways in the angiogenic activities of calycosin. We present a comprehensive framework of study using fluorescence microscopy, transcriptomics and qPCR to demonstrate the proangiogenic effects of calycosin in vivo. The data have elucidated the connection between morphological observations and genomic evidence, indicating the potential roles of several key signaling pathways in angiogenesis. This journal is © The Royal Society of Chemistry. |
| ISSN | 1742-206X 2011 Impact Factor: 3.534 2011 SCImago Journal Rankings: 0.522 |
| DOI | http://dx.doi.org/10.1039/c1mb05206c |
| References | References in Scopus |
| dc.contributor.author | Li, S |
|---|---|
| dc.contributor.author | Lou, S |
| dc.contributor.author | Lei, BUW |
| dc.contributor.author | Chan, TF |
| dc.contributor.author | Kwan, YW |
| dc.contributor.author | Chan, SW |
| dc.contributor.author | Leung, GPH |
| dc.contributor.author | Tsui, SKW |
| dc.contributor.author | Lee, SMY |
| dc.date.accessioned | 2012-08-16T05:56:13Z |
| dc.date.available | 2012-08-16T05:56:13Z |
| dc.date.issued | 2011 |
| dc.description.abstract | Angiogenesis plays an important role in a wide range of physiological processes and many diseases are associated with the dysregulation of angiogenesis. The commonly used Chinese herbal medicine Radix Astragali (known as Huang qi in Chinese) is a potential candidate for treating this type of disease. Calycosin, a major isoflavonoid in Radix Astragali, was identified in our earlier study and shown to induce angiogenesis in human umbilical vein endothelial cells (HUVEC) in vitro and in zebrafish embryos in vivo. Using zebrafish as a testing model, we investigated the angiogenic effect of calycosin on the subintestinal vessels (SIVs) in zebrafish embryos. Our findings using transcriptional profiling by deep sequencing, and confirmed by quantitative real-time PCR (qPCR), demonstrate that calycosin modulated vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and ErbB signaling pathways. The inhibitory effects of calycosin-induced phenotypic responses by several pathway-specific inhibitors (VRI, SU5402, MEK1/2 Inhibitor, Wortmannin and LY294002) further identified the potential involvement of VEGF(R) and FGF(R) signaling pathways in the angiogenic activities of calycosin. We present a comprehensive framework of study using fluorescence microscopy, transcriptomics and qPCR to demonstrate the proangiogenic effects of calycosin in vivo. The data have elucidated the connection between morphological observations and genomic evidence, indicating the potential roles of several key signaling pathways in angiogenesis. This journal is © The Royal Society of Chemistry. |
| dc.description.nature | Link_to_subscribed_fulltext |
| dc.identifier.citation | Molecular Biosystems, 2011, v. 7 n. 11, p. 3112-3121 [How to Cite?] DOI: http://dx.doi.org/10.1039/c1mb05206c |
| dc.identifier.doi | http://dx.doi.org/10.1039/c1mb05206c |
| dc.identifier.epage | 3121 |
| dc.identifier.hkuros | 203459 |
| dc.identifier.issn | 1742-206X 2011 Impact Factor: 3.534 2011 SCImago Journal Rankings: 0.522 |
| dc.identifier.issue | 11 |
| dc.identifier.pmid | 21909574 |
| dc.identifier.scopus | eid_2-s2.0-80054026500 |
| dc.identifier.spage | 3112 |
| dc.identifier.uri | http://hdl.handle.net/10722/159767 |
| dc.identifier.volume | 7 |
| dc.language | eng |
| dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/is/journals/current/mbs/mbspub.htm |
| dc.publisher.place | United Kingdom |
| dc.relation.ispartof | Molecular BioSystems |
| dc.relation.references | References in Scopus |
| dc.title | Transcriptional profiling of angiogenesis activities of calycosin in zebrafish |
| dc.type | Article |
Author Affiliations
- The University of Hong Kong
- University of Macau
- Hong Kong Polytechnic University
- Chinese University of Hong Kong