Article: Molecular characterization of the 2011 Hong Kong scarlet fever outbreak

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TitleMolecular characterization of the 2011 Hong Kong scarlet fever outbreak
AuthorsTse, H4
Bao, JYJ4
Davies, MR2 5
Maamary, P2
Tsoi, HW4
Tong, AHY4
Ho, TCC4
Lin, CH4
Gillen, CM2
Barnett, TC2
Chen, JHK4
Lee, M4
Yam, WC4
Wong, CK4
Ong, CLY2
Chan, YW4
Wu, CW4
Ng, T3
Lim, WWL3
Tsang, THF3
Tse, CWS1
Dougan, G5
Walker, MJ2
Lok, S4
Yuen, KY4
Issue Date2012
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
CitationJournal Of Infectious Diseases, 2012, v. 206 n. 3, p. 341-351 [How to Cite?]
DOI: http://dx.doi.org/10.1093/infdis/jis362
AbstractA scarlet fever outbreak occurred in Hong Kong in 2011. The majority of cases resulted in the isolation of Streptococcus pyogenes emm12 with multiple antibiotic resistances. Phylogenetic analysis of 22 emm12 scarlet fever outbreak isolates, 7 temporally and geographically matched emm12 non-scarlet fever isolates, and 18 emm12 strains isolated during 2005-2010 indicated the outbreak was multiclonal. Genome sequencing of 2 nonclonal scarlet fever isolates (HKU16 and HKU30), coupled with diagnostic polymerase chain reaction assays, identified 2 mobile genetic elements distributed across the major lineages: a 64.9-kb integrative and conjugative element encoding tetracycline and macrolide resistance and a 46.4-kb prophage encoding superantigens SSA and SpeC and the DNase Spd1. Phenotypic comparison of HKU16 and HKU30 with the S. pyogenes M1T1 strain 5448 revealed that HKU16 displays increased adherence to HEp-2 human epithelial cells, whereas HKU16, HKU30, and 5448 exhibit equivalent resistance to neutrophils and virulence in a humanized plasminogen murine model. However, in contrast to M1T1, the virulence of HKU16 and HKU30 was not associated with covRS mutation. The multiclonal nature of the emm12 scarlet fever isolates suggests that factors such as mobile genetic elements, environmental factors, and host immune status may have contributed to the 2011 scarlet fever outbreak. © 2012 The Author.
ISSN0022-1899
2011 Impact Factor: 6.41
2011 SCImago Journal Rankings: 0.831
DOIhttp://dx.doi.org/10.1093/infdis/jis362
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTse, H
dc.contributor.authorBao, JYJ
dc.contributor.authorDavies, MR
dc.contributor.authorMaamary, P
dc.contributor.authorTsoi, HW
dc.contributor.authorTong, AHY
dc.contributor.authorHo, TCC
dc.contributor.authorLin, CH
dc.contributor.authorGillen, CM
dc.contributor.authorBarnett, TC
dc.contributor.authorChen, JHK
dc.contributor.authorLee, M
dc.contributor.authorYam, WC
dc.contributor.authorWong, CK
dc.contributor.authorOng, CLY
dc.contributor.authorChan, YW
dc.contributor.authorWu, CW
dc.contributor.authorNg, T
dc.contributor.authorLim, WWL
dc.contributor.authorTsang, THF
dc.contributor.authorTse, CWS
dc.contributor.authorDougan, G
dc.contributor.authorWalker, MJ
dc.contributor.authorLok, S
dc.contributor.authorYuen, KY
dc.date.accessioned2012-08-16T05:55:03Z
dc.date.available2012-08-16T05:55:03Z
dc.date.issued2012
dc.description.abstractA scarlet fever outbreak occurred in Hong Kong in 2011. The majority of cases resulted in the isolation of Streptococcus pyogenes emm12 with multiple antibiotic resistances. Phylogenetic analysis of 22 emm12 scarlet fever outbreak isolates, 7 temporally and geographically matched emm12 non-scarlet fever isolates, and 18 emm12 strains isolated during 2005-2010 indicated the outbreak was multiclonal. Genome sequencing of 2 nonclonal scarlet fever isolates (HKU16 and HKU30), coupled with diagnostic polymerase chain reaction assays, identified 2 mobile genetic elements distributed across the major lineages: a 64.9-kb integrative and conjugative element encoding tetracycline and macrolide resistance and a 46.4-kb prophage encoding superantigens SSA and SpeC and the DNase Spd1. Phenotypic comparison of HKU16 and HKU30 with the S. pyogenes M1T1 strain 5448 revealed that HKU16 displays increased adherence to HEp-2 human epithelial cells, whereas HKU16, HKU30, and 5448 exhibit equivalent resistance to neutrophils and virulence in a humanized plasminogen murine model. However, in contrast to M1T1, the virulence of HKU16 and HKU30 was not associated with covRS mutation. The multiclonal nature of the emm12 scarlet fever isolates suggests that factors such as mobile genetic elements, environmental factors, and host immune status may have contributed to the 2011 scarlet fever outbreak. © 2012 The Author.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Infectious Diseases, 2012, v. 206 n. 3, p. 341-351 [How to Cite?]
DOI: http://dx.doi.org/10.1093/infdis/jis362
dc.identifier.citeulike10996854
dc.identifier.doihttp://dx.doi.org/10.1093/infdis/jis362
dc.identifier.epage351
dc.identifier.hkuros204613
dc.identifier.issn0022-1899
2011 Impact Factor: 6.41
2011 SCImago Journal Rankings: 0.831
dc.identifier.issue3
dc.identifier.pmid22615319
dc.identifier.scopuseid_2-s2.0-84863908165
dc.identifier.spage341
dc.identifier.urihttp://hdl.handle.net/10722/159735
dc.identifier.volume206
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Infectious Diseases
dc.relation.referencesReferences in Scopus
dc.titleMolecular characterization of the 2011 Hong Kong scarlet fever outbreak
dc.typeArticle
Author Affiliations
  1. Kwong Wah Hospital
  2. University of Queensland
  3. Centre for Health Protection
  4. The University of Hong Kong
  5. Wellcome Trust Sanger Institute