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Article: Molecular characterization of the 2011 Hong Kong scarlet fever outbreak
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TitleMolecular characterization of the 2011 Hong Kong scarlet fever outbreak
 
AuthorsTse, H4
Bao, JYJ4
Davies, MR2 5
Maamary, P2
Tsoi, HW4
Tong, AHY4
Ho, TCC4
Lin, CH4
Gillen, CM2
Barnett, TC2
Chen, JHK4
Lee, M4
Yam, WC4
Wong, CK4
Ong, CLY2
Chan, YW4
Wu, CW4
Ng, T3
Lim, WWL3
Tsang, THF3
Tse, CWS1
Dougan, G5
Walker, MJ2
Lok, S4
Yuen, KY4
 
Issue Date2012
 
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
 
CitationJournal Of Infectious Diseases, 2012, v. 206 n. 3, p. 341-351 [How to Cite?]
DOI: http://dx.doi.org/10.1093/infdis/jis362
 
AbstractA scarlet fever outbreak occurred in Hong Kong in 2011. The majority of cases resulted in the isolation of Streptococcus pyogenes emm12 with multiple antibiotic resistances. Phylogenetic analysis of 22 emm12 scarlet fever outbreak isolates, 7 temporally and geographically matched emm12 non-scarlet fever isolates, and 18 emm12 strains isolated during 2005-2010 indicated the outbreak was multiclonal. Genome sequencing of 2 nonclonal scarlet fever isolates (HKU16 and HKU30), coupled with diagnostic polymerase chain reaction assays, identified 2 mobile genetic elements distributed across the major lineages: a 64.9-kb integrative and conjugative element encoding tetracycline and macrolide resistance and a 46.4-kb prophage encoding superantigens SSA and SpeC and the DNase Spd1. Phenotypic comparison of HKU16 and HKU30 with the S. pyogenes M1T1 strain 5448 revealed that HKU16 displays increased adherence to HEp-2 human epithelial cells, whereas HKU16, HKU30, and 5448 exhibit equivalent resistance to neutrophils and virulence in a humanized plasminogen murine model. However, in contrast to M1T1, the virulence of HKU16 and HKU30 was not associated with covRS mutation. The multiclonal nature of the emm12 scarlet fever isolates suggests that factors such as mobile genetic elements, environmental factors, and host immune status may have contributed to the 2011 scarlet fever outbreak. © 2012 The Author.
 
ISSN0022-1899
2012 Impact Factor: 5.848
2012 SCImago Journal Rankings: 2.723
 
DOIhttp://dx.doi.org/10.1093/infdis/jis362
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTse, H
 
dc.contributor.authorBao, JYJ
 
dc.contributor.authorDavies, MR
 
dc.contributor.authorMaamary, P
 
dc.contributor.authorTsoi, HW
 
dc.contributor.authorTong, AHY
 
dc.contributor.authorHo, TCC
 
dc.contributor.authorLin, CH
 
dc.contributor.authorGillen, CM
 
dc.contributor.authorBarnett, TC
 
dc.contributor.authorChen, JHK
 
dc.contributor.authorLee, M
 
dc.contributor.authorYam, WC
 
dc.contributor.authorWong, CK
 
dc.contributor.authorOng, CLY
 
dc.contributor.authorChan, YW
 
dc.contributor.authorWu, CW
 
dc.contributor.authorNg, T
 
dc.contributor.authorLim, WWL
 
dc.contributor.authorTsang, THF
 
dc.contributor.authorTse, CWS
 
dc.contributor.authorDougan, G
 
dc.contributor.authorWalker, MJ
 
dc.contributor.authorLok, S
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2012-08-16T05:55:03Z
 
dc.date.available2012-08-16T05:55:03Z
 
dc.date.issued2012
 
dc.description.abstractA scarlet fever outbreak occurred in Hong Kong in 2011. The majority of cases resulted in the isolation of Streptococcus pyogenes emm12 with multiple antibiotic resistances. Phylogenetic analysis of 22 emm12 scarlet fever outbreak isolates, 7 temporally and geographically matched emm12 non-scarlet fever isolates, and 18 emm12 strains isolated during 2005-2010 indicated the outbreak was multiclonal. Genome sequencing of 2 nonclonal scarlet fever isolates (HKU16 and HKU30), coupled with diagnostic polymerase chain reaction assays, identified 2 mobile genetic elements distributed across the major lineages: a 64.9-kb integrative and conjugative element encoding tetracycline and macrolide resistance and a 46.4-kb prophage encoding superantigens SSA and SpeC and the DNase Spd1. Phenotypic comparison of HKU16 and HKU30 with the S. pyogenes M1T1 strain 5448 revealed that HKU16 displays increased adherence to HEp-2 human epithelial cells, whereas HKU16, HKU30, and 5448 exhibit equivalent resistance to neutrophils and virulence in a humanized plasminogen murine model. However, in contrast to M1T1, the virulence of HKU16 and HKU30 was not associated with covRS mutation. The multiclonal nature of the emm12 scarlet fever isolates suggests that factors such as mobile genetic elements, environmental factors, and host immune status may have contributed to the 2011 scarlet fever outbreak. © 2012 The Author.
 
dc.description.natureLink_to_OA_fulltext
 
dc.identifier.citationJournal Of Infectious Diseases, 2012, v. 206 n. 3, p. 341-351 [How to Cite?]
DOI: http://dx.doi.org/10.1093/infdis/jis362
 
dc.identifier.citeulike10996854
 
dc.identifier.doihttp://dx.doi.org/10.1093/infdis/jis362
 
dc.identifier.eissn1537-6613
 
dc.identifier.epage351
 
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dc.identifier.issn0022-1899
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2012 SCImago Journal Rankings: 2.723
 
dc.identifier.issue3
 
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dc.identifier.spage341
 
dc.identifier.urihttp://hdl.handle.net/10722/159735
 
dc.identifier.volume206
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Infectious Diseases
 
dc.relation.referencesReferences in Scopus
 
dc.titleMolecular characterization of the 2011 Hong Kong scarlet fever outbreak
 
dc.typeArticle
 
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Author Affiliations
  1. Kwong Wah Hospital
  2. University of Queensland
  3. Centre for Health Protection
  4. The University of Hong Kong
  5. Wellcome Trust Sanger Institute