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Article: Molecular characterization of the 2011 Hong Kong scarlet fever outbreak

TitleMolecular characterization of the 2011 Hong Kong scarlet fever outbreak
Authors
Issue Date2012
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
Citation
Journal Of Infectious Diseases, 2012, v. 206 n. 3, p. 341-351 How to Cite?
AbstractA scarlet fever outbreak occurred in Hong Kong in 2011. The majority of cases resulted in the isolation of Streptococcus pyogenes emm12 with multiple antibiotic resistances. Phylogenetic analysis of 22 emm12 scarlet fever outbreak isolates, 7 temporally and geographically matched emm12 non-scarlet fever isolates, and 18 emm12 strains isolated during 2005-2010 indicated the outbreak was multiclonal. Genome sequencing of 2 nonclonal scarlet fever isolates (HKU16 and HKU30), coupled with diagnostic polymerase chain reaction assays, identified 2 mobile genetic elements distributed across the major lineages: a 64.9-kb integrative and conjugative element encoding tetracycline and macrolide resistance and a 46.4-kb prophage encoding superantigens SSA and SpeC and the DNase Spd1. Phenotypic comparison of HKU16 and HKU30 with the S. pyogenes M1T1 strain 5448 revealed that HKU16 displays increased adherence to HEp-2 human epithelial cells, whereas HKU16, HKU30, and 5448 exhibit equivalent resistance to neutrophils and virulence in a humanized plasminogen murine model. However, in contrast to M1T1, the virulence of HKU16 and HKU30 was not associated with covRS mutation. The multiclonal nature of the emm12 scarlet fever isolates suggests that factors such as mobile genetic elements, environmental factors, and host immune status may have contributed to the 2011 scarlet fever outbreak. © 2012 The Author.
Persistent Identifierhttp://hdl.handle.net/10722/159735
ISSN
2015 Impact Factor: 6.344
2015 SCImago Journal Rankings: 4.000
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, Hen_HK
dc.contributor.authorBao, JYJen_HK
dc.contributor.authorDavies, MRen_HK
dc.contributor.authorMaamary, Pen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorTong, AHYen_HK
dc.contributor.authorHo, TCCen_HK
dc.contributor.authorLin, CHen_HK
dc.contributor.authorGillen, CMen_HK
dc.contributor.authorBarnett, TCen_HK
dc.contributor.authorChen, JHKen_HK
dc.contributor.authorLee, Men_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorWong, CKen_HK
dc.contributor.authorOng, CLYen_HK
dc.contributor.authorChan, YWen_HK
dc.contributor.authorWu, CWen_HK
dc.contributor.authorNg, Ten_HK
dc.contributor.authorLim, WWLen_HK
dc.contributor.authorTsang, THFen_HK
dc.contributor.authorTse, CWSen_HK
dc.contributor.authorDougan, Gen_HK
dc.contributor.authorWalker, MJen_HK
dc.contributor.authorLok, Sen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2012-08-16T05:55:03Z-
dc.date.available2012-08-16T05:55:03Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Infectious Diseases, 2012, v. 206 n. 3, p. 341-351en_HK
dc.identifier.issn0022-1899en_HK
dc.identifier.urihttp://hdl.handle.net/10722/159735-
dc.description.abstractA scarlet fever outbreak occurred in Hong Kong in 2011. The majority of cases resulted in the isolation of Streptococcus pyogenes emm12 with multiple antibiotic resistances. Phylogenetic analysis of 22 emm12 scarlet fever outbreak isolates, 7 temporally and geographically matched emm12 non-scarlet fever isolates, and 18 emm12 strains isolated during 2005-2010 indicated the outbreak was multiclonal. Genome sequencing of 2 nonclonal scarlet fever isolates (HKU16 and HKU30), coupled with diagnostic polymerase chain reaction assays, identified 2 mobile genetic elements distributed across the major lineages: a 64.9-kb integrative and conjugative element encoding tetracycline and macrolide resistance and a 46.4-kb prophage encoding superantigens SSA and SpeC and the DNase Spd1. Phenotypic comparison of HKU16 and HKU30 with the S. pyogenes M1T1 strain 5448 revealed that HKU16 displays increased adherence to HEp-2 human epithelial cells, whereas HKU16, HKU30, and 5448 exhibit equivalent resistance to neutrophils and virulence in a humanized plasminogen murine model. However, in contrast to M1T1, the virulence of HKU16 and HKU30 was not associated with covRS mutation. The multiclonal nature of the emm12 scarlet fever isolates suggests that factors such as mobile genetic elements, environmental factors, and host immune status may have contributed to the 2011 scarlet fever outbreak. © 2012 The Author.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.orgen_HK
dc.relation.ispartofJournal of Infectious Diseasesen_HK
dc.titleMolecular characterization of the 2011 Hong Kong scarlet fever outbreaken_HK
dc.typeArticleen_HK
dc.identifier.emailTse, H: htse@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW: hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYam, WC: wcyam@hkucc.hku.hken_HK
dc.identifier.emailLok, S: silok@genome.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/infdis/jis362en_HK
dc.identifier.pmid22615319-
dc.identifier.scopuseid_2-s2.0-84863908165en_HK
dc.identifier.hkuros204613en_US
dc.identifier.hkuros225931-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863908165&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume206en_HK
dc.identifier.issue3en_HK
dc.identifier.spage341en_HK
dc.identifier.epage351en_HK
dc.identifier.eissn1537-6613-
dc.identifier.isiWOS:000306369100006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridBao, JYJ=45960893700en_HK
dc.identifier.scopusauthoridDavies, MR=7404208351en_HK
dc.identifier.scopusauthoridMaamary, P=24492021100en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridTong, AHY=55314966100en_HK
dc.identifier.scopusauthoridHo, TCC=50561201300en_HK
dc.identifier.scopusauthoridLin, CH=44761160100en_HK
dc.identifier.scopusauthoridGillen, CM=7004018682en_HK
dc.identifier.scopusauthoridBarnett, TC=8090628100en_HK
dc.identifier.scopusauthoridChen, JHK=35085819900en_HK
dc.identifier.scopusauthoridLee, M=55315195000en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridWong, CK=55315307200en_HK
dc.identifier.scopusauthoridOng, CLY=23480460500en_HK
dc.identifier.scopusauthoridChan, YW=7403676348en_HK
dc.identifier.scopusauthoridWu, CW=55316088000en_HK
dc.identifier.scopusauthoridNg, T=55315932900en_HK
dc.identifier.scopusauthoridLim, WWL=7202378267en_HK
dc.identifier.scopusauthoridTsang, THF=7101832378en_HK
dc.identifier.scopusauthoridTse, CWS=7103295064en_HK
dc.identifier.scopusauthoridDougan, G=7102366949en_HK
dc.identifier.scopusauthoridWalker, MJ=34974875300en_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike10996854-

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