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Article: Parental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC

TitleParental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BC
Authors
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS ONE, 2012, v. 7 n. 2, article no. e31233 How to Cite?
AbstractCirculating recombinant forms (CRFs) of HIV-1 have been identified in southern China in recent years. CRF08_BC is one of the most predominant subtypes circulating in China. In order to study HIV subtype biology and to provide a tool for biotechnological applications, the first full-length replication-competent infectious molecular clone harboring CRF08_BC is reported. The construction of this clone pBRGX indicates that a moderate-copy number vector is required for its amplification in E. coli. In addition, it is shown that the parental CRF08_BC LTRs are important for generating this efficient replication-competent infectious clone. These observations may aid in the construction of infectious clones from other subtypes. Both the pBRGX-derived virus and its parental isolate contain CCR5 tropism. Their full-length genomes were also sequenced, analyzed, compared and deposited in GenBank (JF719819 and JF719818, respectively). The availability of pBRGX as the first replication-competent molecular clone of CRF08_BC provides a useful tool for a wide range of studies of this newly emergent HIV subtype, including the development of HIV vaccine candidates, antiviral drug screening and drug resistance analysis.
Persistent Identifierhttp://hdl.handle.net/10722/159712
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, Qen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorWu, Hen_US
dc.contributor.authorSeto, Den_US
dc.contributor.authorZhang, HJen_US
dc.contributor.authorChen, Zen_US
dc.contributor.authorWan, Cen_US
dc.contributor.authorZheng, BJen_US
dc.date.accessioned2012-08-16T05:54:50Z-
dc.date.available2012-08-16T05:54:50Z-
dc.date.issued2012en_US
dc.identifier.citationPLoS ONE, 2012, v. 7 n. 2, article no. e31233en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/159712-
dc.description.abstractCirculating recombinant forms (CRFs) of HIV-1 have been identified in southern China in recent years. CRF08_BC is one of the most predominant subtypes circulating in China. In order to study HIV subtype biology and to provide a tool for biotechnological applications, the first full-length replication-competent infectious molecular clone harboring CRF08_BC is reported. The construction of this clone pBRGX indicates that a moderate-copy number vector is required for its amplification in E. coli. In addition, it is shown that the parental CRF08_BC LTRs are important for generating this efficient replication-competent infectious clone. These observations may aid in the construction of infectious clones from other subtypes. Both the pBRGX-derived virus and its parental isolate contain CCR5 tropism. Their full-length genomes were also sequenced, analyzed, compared and deposited in GenBank (JF719819 and JF719818, respectively). The availability of pBRGX as the first replication-competent molecular clone of CRF08_BC provides a useful tool for a wide range of studies of this newly emergent HIV subtype, including the development of HIV vaccine candidates, antiviral drug screening and drug resistance analysis.-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS ONEen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAmino Acid Substitution - genetics-
dc.subject.meshDNA, Viral - genetics-
dc.subject.meshHIV-1 - classification - genetics - physiology-
dc.subject.meshTerminal Repeat Sequences - genetics-
dc.subject.meshVirus Replication - genetics-
dc.titleParental LTRs are important in a construct of a stable and efficient replication-competent infectious molecular clone of HIV-1 CRF08_BCen_US
dc.typeArticleen_US
dc.identifier.emailZhang, Q: zhangqw@hkucc.hku.hk, zhang.qiwei@yahoo.comen_US
dc.identifier.emailZhang, HJ: zhanghj@hku.hken_US
dc.identifier.emailChen, Z: zchenai@hku.hk-
dc.identifier.emailZheng, BJ: bzheng@hkucc.hku.hk-
dc.identifier.authorityChen, Z=rp00243en_US
dc.identifier.authorityZheng, B=rp00353en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0031233-
dc.identifier.pmid22363589-
dc.identifier.pmcidPMC3281951-
dc.identifier.scopuseid_2-s2.0-84857170595-
dc.identifier.hkuros206305en_US
dc.identifier.volume7en_US
dc.identifier.issue2, article no. e31233en_US
dc.identifier.isiWOS:000302853600127-
dc.publisher.placeUnited States-

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