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Article: Electrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions
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TitleElectrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions
 
AuthorsTong, HW1
Wang, M1
Lu, WW1
 
KeywordsElectrospinning
Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)
Tissue-engineering scaffold
Surface-porous fibre
Composite fibre
 
Issue Date2012
 
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
 
CitationJournal of Biomaterials Science Polymer Edition, 2012, v. 23 n. 6, p. 779-806 [How to Cite?]
DOI: http://dx.doi.org/10.1163/092050611X560708
 
AbstractWhile electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA nanoparticles in microfibres significantly enhanced the alkaline phosphatase activity of SaOS-2 cells. The control of fibre diameter, surface topography and composition is important in developing electrospun PHBV-based scaffolds for specific tissue-engineering applications.
 
ISSN0920-5063
2013 Impact Factor: 1.357
 
DOIhttp://dx.doi.org/10.1163/092050611X560708
 
ISI Accession Number IDWOS:000300651300004
 
DC FieldValue
dc.contributor.authorTong, HW
 
dc.contributor.authorWang, M
 
dc.contributor.authorLu, WW
 
dc.date.accessioned2012-08-16T05:52:39Z
 
dc.date.available2012-08-16T05:52:39Z
 
dc.date.issued2012
 
dc.description.abstractWhile electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA nanoparticles in microfibres significantly enhanced the alkaline phosphatase activity of SaOS-2 cells. The control of fibre diameter, surface topography and composition is important in developing electrospun PHBV-based scaffolds for specific tissue-engineering applications.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal of Biomaterials Science Polymer Edition, 2012, v. 23 n. 6, p. 779-806 [How to Cite?]
DOI: http://dx.doi.org/10.1163/092050611X560708
 
dc.identifier.doihttp://dx.doi.org/10.1163/092050611X560708
 
dc.identifier.epage806
 
dc.identifier.hkuros204404
 
dc.identifier.isiWOS:000300651300004
 
dc.identifier.issn0920-5063
2013 Impact Factor: 1.357
 
dc.identifier.issue6
 
dc.identifier.scopuseid_2-s2.0-84863229338
 
dc.identifier.spage779
 
dc.identifier.urihttp://hdl.handle.net/10722/159568
 
dc.identifier.volume23
 
dc.languageeng
 
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Biomaterials Science Polymer Edition
 
dc.subjectElectrospinning
 
dc.subjectPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)
 
dc.subjectTissue-engineering scaffold
 
dc.subjectSurface-porous fibre
 
dc.subjectComposite fibre
 
dc.titleElectrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions
 
dc.typeArticle
 
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<item><contributor.author>Tong, HW</contributor.author>
<contributor.author>Wang, M</contributor.author>
<contributor.author>Lu, WW</contributor.author>
<date.accessioned>2012-08-16T05:52:39Z</date.accessioned>
<date.available>2012-08-16T05:52:39Z</date.available>
<date.issued>2012</date.issued>
<identifier.citation>Journal of Biomaterials Science Polymer Edition, 2012, v. 23 n. 6, p. 779-806</identifier.citation>
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<description.abstract>While electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA nanoparticles in microfibres significantly enhanced the alkaline phosphatase activity of SaOS-2 cells. The control of fibre diameter, surface topography and composition is important in developing electrospun PHBV-based scaffolds for specific tissue-engineering applications.</description.abstract>
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<subject>Electrospinning</subject>
<subject>Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)</subject>
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Author Affiliations
  1. The University of Hong Kong