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Article: Electrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions

TitleElectrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions
Authors
KeywordsElectrospinning
Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)
Tissue-engineering scaffold
Surface-porous fibre
Composite fibre
Issue Date2012
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
Citation
Journal of Biomaterials Science Polymer Edition, 2012, v. 23 n. 6, p. 779-806 How to Cite?
AbstractWhile electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA nanoparticles in microfibres significantly enhanced the alkaline phosphatase activity of SaOS-2 cells. The control of fibre diameter, surface topography and composition is important in developing electrospun PHBV-based scaffolds for specific tissue-engineering applications.
Persistent Identifierhttp://hdl.handle.net/10722/159568
ISSN
2014 Impact Factor: 1.648
2014 SCImago Journal Rankings: 0.496
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTong, HWen_US
dc.contributor.authorWang, Men_US
dc.contributor.authorLu, WWen_US
dc.date.accessioned2012-08-16T05:52:39Z-
dc.date.available2012-08-16T05:52:39Z-
dc.date.issued2012en_US
dc.identifier.citationJournal of Biomaterials Science Polymer Edition, 2012, v. 23 n. 6, p. 779-806en_US
dc.identifier.issn0920-5063-
dc.identifier.urihttp://hdl.handle.net/10722/159568-
dc.description.abstractWhile electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA nanoparticles in microfibres significantly enhanced the alkaline phosphatase activity of SaOS-2 cells. The control of fibre diameter, surface topography and composition is important in developing electrospun PHBV-based scaffolds for specific tissue-engineering applications.-
dc.languageengen_US
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current-
dc.relation.ispartofJournal of Biomaterials Science Polymer Editionen_US
dc.subjectElectrospinning-
dc.subjectPoly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)-
dc.subjectTissue-engineering scaffold-
dc.subjectSurface-porous fibre-
dc.subjectComposite fibre-
dc.titleElectrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositionsen_US
dc.typeArticleen_US
dc.identifier.emailTong, HW: meboris@hku.hken_US
dc.identifier.emailWang, M: memwang@hku.hken_US
dc.identifier.emailLu, WW: wwlu@hku.hken_US
dc.identifier.authorityWang, M=rp00185en_US
dc.identifier.authorityLu, WW=rp00411en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1163/092050611X560708-
dc.identifier.scopuseid_2-s2.0-84863229338-
dc.identifier.hkuros204404en_US
dc.identifier.volume23en_US
dc.identifier.issue6-
dc.identifier.spage779en_US
dc.identifier.epage806en_US
dc.identifier.isiWOS:000300651300004-
dc.publisher.placeUnited Kingdom-

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