Article: Electrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions

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Publisher Website: 10.1163/092050611X560708
Scopus: eid_2-s2.0-84863229338

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Title	Electrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions
Authors	Tong, HW1 Wang, M1 Lu, WW1
Keywords	Electrospinning Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) Tissue-engineering scaffold Surface-porous fibre Composite fibre
Issue Date	2012
Publisher	Taylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
Citation	Journal of Biomaterials Science Polymer Edition, 2012, v. 23 n. 6, p. 779-806 [How to Cite?] DOI: http://dx.doi.org/10.1163/092050611X560708
Abstract	While electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA nanoparticles in microfibres significantly enhanced the alkaline phosphatase activity of SaOS-2 cells. The control of fibre diameter, surface topography and composition is important in developing electrospun PHBV-based scaffolds for specific tissue-engineering applications.
ISSN	0920-5063 2011 Impact Factor: 1.691 2011 SCImago Journal Rankings: 0.147
DOI	http://dx.doi.org/10.1163/092050611X560708
ISI Accession Number ID	WOS:000300651300004

DC Field	Value
dc.contributor.author	Tong, HW
dc.contributor.author	Wang, M
dc.contributor.author	Lu, WW
dc.date.accessioned	2012-08-16T05:52:39Z
dc.date.available	2012-08-16T05:52:39Z
dc.date.issued	2012
dc.description.abstract	While electrospinning is an effective technology for producing poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) micrometre-scale fibrous scaffolds for tissue regeneration, electrospinning of PHBV fibrous scaffolds composed of sub-micrometre fibres, surface-porous fibres or nanocomposite fibres is rarely explored. In this study, the average PHBV fibre diameter was successfully reduced to the sub-micrometre scale by dissolving a conductivity-enhancing salt in the polymer solution for electrospinning. Surface-porous fibres were made using a mixture of solvents, and carbonated hydroxyapatite (CHA) nanoparticles were incorporated into the fibres with the aid of an ultrasonic power source. Water contact angle measurements demonstrated that both fibre diameter reduction and CHA incorporation enhanced the wettability of the fibrous scaffolds. Tensile properties of the scaffolds were not undermined by the reduction of fibre diameter and the presence of surface pores. In vitro biological evaluation using a human osteoblast-like cell line (SaOS-2) demonstrated that all types of fibrous scaffolds supported cell attachment, spreading and proliferation. Analysis of cell morphology revealed similar projected cell areas on all types of scaffolds. However, cells on sub-micrometre fibres possessed a lower cell aspect ratio than cells on microfibres. The reduction of fibre diameter to the sub-micrometre scale enhanced cell proliferation after 14 days cell culture, while the incorporation of CHA nanoparticles in microfibres significantly enhanced the alkaline phosphatase activity of SaOS-2 cells. The control of fibre diameter, surface topography and composition is important in developing electrospun PHBV-based scaffolds for specific tissue-engineering applications.
dc.description.nature	Link_to_subscribed_fulltext
dc.identifier.citation	Journal of Biomaterials Science Polymer Edition, 2012, v. 23 n. 6, p. 779-806 [How to Cite?] DOI: http://dx.doi.org/10.1163/092050611X560708
dc.identifier.doi	http://dx.doi.org/10.1163/092050611X560708
dc.identifier.epage	806
dc.identifier.hkuros	204404
dc.identifier.isi	WOS:000300651300004
dc.identifier.issn	0920-5063 2011 Impact Factor: 1.691 2011 SCImago Journal Rankings: 0.147
dc.identifier.issue	6
dc.identifier.scopus	eid_2-s2.0-84863229338
dc.identifier.spage	779
dc.identifier.uri	http://hdl.handle.net/10722/159568
dc.identifier.volume	23
dc.language	eng
dc.publisher	Taylor & Francis Ltd. The Journal's web site is located at http://www.tandfonline.com/toc/tbsp20/current
dc.publisher.place	United Kingdom
dc.relation.ispartof	Journal of Biomaterials Science Polymer Edition
dc.subject	Electrospinning
dc.subject	Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV)
dc.subject	Tissue-engineering scaffold
dc.subject	Surface-porous fibre
dc.subject	Composite fibre
dc.title	Electrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions
dc.type	Article

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The University of Hong Kong

Article: Electrospinning and evaluation of PHBV-based tissue engineering scaffolds with different fibre diameters, surface topography and compositions

The HKU Scholars Hub has contact details for these author(s).