File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Folic acid conjugated mPEG-PEI600 as an efficient non-viral vector for targeted nucleic acid delivery
  • Basic View
  • Metadata View
  • XML View
TitleFolic acid conjugated mPEG-PEI600 as an efficient non-viral vector for targeted nucleic acid delivery
 
AuthorsXu, Z1 2 3
Jin, J1
Siu, LKS1
Yao, H2 3
Sze, J2
Sun, H1
Kung, HF3
Poon, WS2
Ng, SSM1 1
Lin, MC1 2
 
KeywordsPEG
Polyethylenimine
Polymer
Tumor gene delivery
 
Issue Date2012
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijpharm
 
CitationInternational Journal Of Pharmaceutics, 2012, v. 426 n. 1-2, p. 182-192 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ijpharm.2012.01.009
 
AbstractIn this study we describe a novel polymer, mPPS-FA, synthesized as a potential gene transfer vector. To complete mPPS-FA, folic acid was conjugated to a backbone (named mPPS) consisting of a copolymer of methyl PEG-2000, PEI-600, and sebacoyl chloride. 1H NMR, FT-IR, and UV spectroscopy were used to characterize the structure of mPPS-FA. It was revealed that mPPS-FA holds the ability to bind plasmid DNA yielding positively charged particles (polyplexes). Dynamic light scattering (DLS) and TEM techniques were used to study the size and morphology of the formed mPPS-FA/DNA nanocomplexes. The mPPS-FA/DNA nanoparticles exhibited low cytotoxicity as transfection of B16-F0, U87MG, CHO-1, and Ho-8910 cells produced >80% viability indicating low cytotoxicity of the polymer. The ability of mPPS-FA to deliver EGFP plasmid to melanoma B16-F0, U87, CHO-1, Ho-8910, and A549 cells was investigated in vitro as compared to the lipid-based transfection agent Lipofectamine™2000 and Linear PEI 22kDa (L-PEI 22kDa). We found that mPPS-FA/DNA complexes yielded the highest GFP transfection efficiency in B16-F0, U87, CHO-1, and Ho-8910 cells, which all highly express folate receptors (FR), at an mPPS-FA/DNA ratio (w/w) of 15. Furthermore, the transfection of mPPS-FA/DNA complexes in CHO-1 cells could be competitively blocked by free folic acid molecules. In contrast, in low FR expressing A549 cells, mPPS-FA showed similar low transfection efficiency as mPPS. Taken together, mPPS-FA showed the highest efficiency in vitro and the potential to be developed as a nonviral gene carrier. © 2012 Elsevier B.V. All rights reserved.
 
ISSN0378-5173
2012 Impact Factor: 3.458
2012 SCImago Journal Rankings: 1.311
 
DOIhttp://dx.doi.org/10.1016/j.ijpharm.2012.01.009
 
ISI Accession Number IDWOS:000302364300021
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorXu, Z
 
dc.contributor.authorJin, J
 
dc.contributor.authorSiu, LKS
 
dc.contributor.authorYao, H
 
dc.contributor.authorSze, J
 
dc.contributor.authorSun, H
 
dc.contributor.authorKung, HF
 
dc.contributor.authorPoon, WS
 
dc.contributor.authorNg, SSM
 
dc.contributor.authorLin, MC
 
dc.date.accessioned2012-08-16T05:48:59Z
 
dc.date.available2012-08-16T05:48:59Z
 
dc.date.issued2012
 
dc.description.abstractIn this study we describe a novel polymer, mPPS-FA, synthesized as a potential gene transfer vector. To complete mPPS-FA, folic acid was conjugated to a backbone (named mPPS) consisting of a copolymer of methyl PEG-2000, PEI-600, and sebacoyl chloride. 1H NMR, FT-IR, and UV spectroscopy were used to characterize the structure of mPPS-FA. It was revealed that mPPS-FA holds the ability to bind plasmid DNA yielding positively charged particles (polyplexes). Dynamic light scattering (DLS) and TEM techniques were used to study the size and morphology of the formed mPPS-FA/DNA nanocomplexes. The mPPS-FA/DNA nanoparticles exhibited low cytotoxicity as transfection of B16-F0, U87MG, CHO-1, and Ho-8910 cells produced >80% viability indicating low cytotoxicity of the polymer. The ability of mPPS-FA to deliver EGFP plasmid to melanoma B16-F0, U87, CHO-1, Ho-8910, and A549 cells was investigated in vitro as compared to the lipid-based transfection agent Lipofectamine™2000 and Linear PEI 22kDa (L-PEI 22kDa). We found that mPPS-FA/DNA complexes yielded the highest GFP transfection efficiency in B16-F0, U87, CHO-1, and Ho-8910 cells, which all highly express folate receptors (FR), at an mPPS-FA/DNA ratio (w/w) of 15. Furthermore, the transfection of mPPS-FA/DNA complexes in CHO-1 cells could be competitively blocked by free folic acid molecules. In contrast, in low FR expressing A549 cells, mPPS-FA showed similar low transfection efficiency as mPPS. Taken together, mPPS-FA showed the highest efficiency in vitro and the potential to be developed as a nonviral gene carrier. © 2012 Elsevier B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationInternational Journal Of Pharmaceutics, 2012, v. 426 n. 1-2, p. 182-192 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ijpharm.2012.01.009
 
dc.identifier.citeulike10280072
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ijpharm.2012.01.009
 
dc.identifier.epage192
 
dc.identifier.hkuros205092
 
dc.identifier.isiWOS:000302364300021
 
dc.identifier.issn0378-5173
2012 Impact Factor: 3.458
2012 SCImago Journal Rankings: 1.311
 
dc.identifier.issue1-2
 
dc.identifier.openurl
 
dc.identifier.pmid22265912
 
dc.identifier.scopuseid_2-s2.0-84862832308
 
dc.identifier.spage182
 
dc.identifier.urihttp://hdl.handle.net/10722/159383
 
dc.identifier.volume426
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijpharm
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofInternational Journal of Pharmaceutics
 
dc.relation.referencesReferences in Scopus
 
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#
 
dc.subject.meshAlkanes - chemistry - toxicity
 
dc.subject.meshAnimals
 
dc.subject.meshBinding, Competitive
 
dc.subject.meshCHO Cells
 
dc.subject.meshCell Survival - drug effects
 
dc.subject.meshCricetinae
 
dc.subject.meshCricetulus
 
dc.subject.meshDNA - chemistry - metabolism
 
dc.subject.meshFolic Acid - chemistry - metabolism - toxicity
 
dc.subject.meshGreen Fluorescent Proteins - biosynthesis - genetics
 
dc.subject.meshHumans
 
dc.subject.meshImines - chemistry - toxicity
 
dc.subject.meshLight
 
dc.subject.meshMagnetic Resonance Spectroscopy
 
dc.subject.meshMethylation
 
dc.subject.meshMice
 
dc.subject.meshMicroscopy, Electron, Transmission
 
dc.subject.meshNanoparticles
 
dc.subject.meshNanotechnology
 
dc.subject.meshParticle Size
 
dc.subject.meshPolyethylene Glycols - chemistry - toxicity
 
dc.subject.meshPolyethylenes - chemistry - toxicity
 
dc.subject.meshScattering, Radiation
 
dc.subject.meshSpectrophotometry, Ultraviolet
 
dc.subject.meshSpectroscopy, Fourier Transform Infrared
 
dc.subject.meshTransfection - methods
 
dc.subjectPEG
 
dc.subjectPolyethylenimine
 
dc.subjectPolymer
 
dc.subjectTumor gene delivery
 
dc.titleFolic acid conjugated mPEG-PEI600 as an efficient non-viral vector for targeted nucleic acid delivery
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Xu, Z</contributor.author>
<contributor.author>Jin, J</contributor.author>
<contributor.author>Siu, LKS</contributor.author>
<contributor.author>Yao, H</contributor.author>
<contributor.author>Sze, J</contributor.author>
<contributor.author>Sun, H</contributor.author>
<contributor.author>Kung, HF</contributor.author>
<contributor.author>Poon, WS</contributor.author>
<contributor.author>Ng, SSM</contributor.author>
<contributor.author>Lin, MC</contributor.author>
<date.accessioned>2012-08-16T05:48:59Z</date.accessioned>
<date.available>2012-08-16T05:48:59Z</date.available>
<date.issued>2012</date.issued>
<identifier.citation>International Journal Of Pharmaceutics, 2012, v. 426 n. 1-2, p. 182-192</identifier.citation>
<identifier.issn>0378-5173</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/159383</identifier.uri>
<description.abstract>In this study we describe a novel polymer, mPPS-FA, synthesized as a potential gene transfer vector. To complete mPPS-FA, folic acid was conjugated to a backbone (named mPPS) consisting of a copolymer of methyl PEG-2000, PEI-600, and sebacoyl chloride. 1H NMR, FT-IR, and UV spectroscopy were used to characterize the structure of mPPS-FA. It was revealed that mPPS-FA holds the ability to bind plasmid DNA yielding positively charged particles (polyplexes). Dynamic light scattering (DLS) and TEM techniques were used to study the size and morphology of the formed mPPS-FA/DNA nanocomplexes. The mPPS-FA/DNA nanoparticles exhibited low cytotoxicity as transfection of B16-F0, U87MG, CHO-1, and Ho-8910 cells produced &gt;80% viability indicating low cytotoxicity of the polymer. The ability of mPPS-FA to deliver EGFP plasmid to melanoma B16-F0, U87, CHO-1, Ho-8910, and A549 cells was investigated in vitro as compared to the lipid-based transfection agent Lipofectamine&#8482;2000 and Linear PEI 22kDa (L-PEI 22kDa). We found that mPPS-FA/DNA complexes yielded the highest GFP transfection efficiency in B16-F0, U87, CHO-1, and Ho-8910 cells, which all highly express folate receptors (FR), at an mPPS-FA/DNA ratio (w/w) of 15. Furthermore, the transfection of mPPS-FA/DNA complexes in CHO-1 cells could be competitively blocked by free folic acid molecules. In contrast, in low FR expressing A549 cells, mPPS-FA showed similar low transfection efficiency as mPPS. Taken together, mPPS-FA showed the highest efficiency in vitro and the potential to be developed as a nonviral gene carrier. &#169; 2012 Elsevier B.V. All rights reserved.</description.abstract>
<language>eng</language>
<publisher>Elsevier BV. The Journal&apos;s web site is located at http://www.elsevier.com/locate/ijpharm</publisher>
<relation.ispartof>International Journal of Pharmaceutics</relation.ispartof>
<rights>NOTICE: this is the author&#8217;s version of a work that was accepted for publication in &lt;Journal title&gt;. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#</rights>
<subject>PEG</subject>
<subject>Polyethylenimine</subject>
<subject>Polymer</subject>
<subject>Tumor gene delivery</subject>
<subject.mesh>Alkanes - chemistry - toxicity</subject.mesh>
<subject.mesh>Animals</subject.mesh>
<subject.mesh>Binding, Competitive</subject.mesh>
<subject.mesh>CHO Cells</subject.mesh>
<subject.mesh>Cell Survival - drug effects</subject.mesh>
<subject.mesh>Cricetinae</subject.mesh>
<subject.mesh>Cricetulus</subject.mesh>
<subject.mesh>DNA - chemistry - metabolism</subject.mesh>
<subject.mesh>Folic Acid - chemistry - metabolism - toxicity</subject.mesh>
<subject.mesh>Green Fluorescent Proteins - biosynthesis - genetics</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Imines - chemistry - toxicity</subject.mesh>
<subject.mesh>Light</subject.mesh>
<subject.mesh>Magnetic Resonance Spectroscopy</subject.mesh>
<subject.mesh>Methylation</subject.mesh>
<subject.mesh>Mice</subject.mesh>
<subject.mesh>Microscopy, Electron, Transmission</subject.mesh>
<subject.mesh>Nanoparticles</subject.mesh>
<subject.mesh>Nanotechnology</subject.mesh>
<subject.mesh>Particle Size</subject.mesh>
<subject.mesh>Polyethylene Glycols - chemistry - toxicity</subject.mesh>
<subject.mesh>Polyethylenes - chemistry - toxicity</subject.mesh>
<subject.mesh>Scattering, Radiation</subject.mesh>
<subject.mesh>Spectrophotometry, Ultraviolet</subject.mesh>
<subject.mesh>Spectroscopy, Fourier Transform Infrared</subject.mesh>
<subject.mesh>Transfection - methods</subject.mesh>
<title>Folic acid conjugated mPEG-PEI600 as an efficient non-viral vector for targeted nucleic acid delivery</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=0378-5173&amp;volume=426&amp;spage=182&amp;epage=192&amp;date=2012&amp;atitle=Folic+acid+conjugated+mPEG-PEI600+as+an+efficient+non-viral+vector+for+targeted+nucleic+acid+delivery</identifier.openurl>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1016/j.ijpharm.2012.01.009</identifier.doi>
<identifier.pmid>22265912</identifier.pmid>
<identifier.scopus>eid_2-s2.0-84862832308</identifier.scopus>
<identifier.hkuros>205092</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-84862832308&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>426</identifier.volume>
<identifier.issue>1-2</identifier.issue>
<identifier.spage>182</identifier.spage>
<identifier.epage>192</identifier.epage>
<identifier.isi>WOS:000302364300021</identifier.isi>
<publisher.place>Netherlands</publisher.place>
<identifier.citeulike>10280072</identifier.citeulike>
</item>
Author Affiliations
  1. The University of Hong Kong
  2. Prince of Wales Hospital Hong Kong
  3. Chinese University of Hong Kong