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Article: Polymer-coated NaYF4:Yb3+, Er3+ upconversion nanoparticles for charge-dependent cellular imaging

TitlePolymer-coated NaYF4:Yb3+, Er3+ upconversion nanoparticles for charge-dependent cellular imaging
Authors
KeywordsCharge-dependent cellular uptake
Clathrin-mediated endocytosis
Ligand exchange
Upconversion nanoparticles
Issue Date2011
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html
Citation
ACS NANO, 2011, v. 5 n. 10, p. 7838-7847 How to Cite?
AbstractLanthanide-doped upconversion nanoparticles (UCNPs) are considered promising novel near-infrared (NIR) bioimaging agents with the characteristics of high contrast and high penetration depth. However, the interactions between charged UCNPs and mammalian cells have not been thoroughly studied, and the corresponding intracellular uptake pathways remain unclear. Herein, our research work involved the use of a hydrothermal method to synthesize polyvinylpyrrolidone-coated UCNPs (UCNP-PVP), and then a ligand exchange reaction was performed on UCNP-PVP, with the help of polyethylenimine (PEI) and poly(acrylic acid) (PAA), to generate UCNP-PEI and UCNP-PAA. These polymer-coated UCNPs demonstrated good dispersibility in aqueous medium, had the same elemental composition and crystal phase, shared similar TEM and dynamic light scattering (DLS) size distribution, and exhibited similar upconversion luminescence efficiency. However, the positively charged UCNP-PEI evinced greatly enhanced cellular uptake in comparison with its neutral or negative counterparts, as shown by multiphoton confocal microscopy and inductively coupled plasma mass spectrometry (ICP-MS) measurements. Meanwhile, we found that cationic UCNP-PEI can be effectively internalized mainly through the clathrin endocytic mechanism, as revealed by colocalization, chemical, and genetic inhibitor studies. This study elucidates the role of the surface polymer coatings in governing UCNP-cell interactions, and it is the first report on the endocytic mechanism of positively charged lanthanide-doped UCNPs. Furthermore, this study provides important guidance for the development of UCNPs as specific intracellular nanoprobes, allowing us to control the UCNP-cell interactions by tuning surface properties.
Persistent Identifierhttp://hdl.handle.net/10722/159321
ISSN
2015 Impact Factor: 13.334
2015 SCImago Journal Rankings: 7.120
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJin, Jen_US
dc.contributor.authorGu, YJen_US
dc.contributor.authorMan, CWYen_US
dc.contributor.authorCheng, Jen_US
dc.contributor.authorXu, Zen_US
dc.contributor.authorZhang, Yen_US
dc.contributor.authorWang, Hen_US
dc.contributor.authorLee, VHYen_US
dc.contributor.authorCheng, SHen_US
dc.contributor.authorWong, WTen_US
dc.date.accessioned2012-08-16T05:48:38Z-
dc.date.available2012-08-16T05:48:38Z-
dc.date.issued2011en_US
dc.identifier.citationACS NANO, 2011, v. 5 n. 10, p. 7838-7847en_US
dc.identifier.issn1936-0851-
dc.identifier.urihttp://hdl.handle.net/10722/159321-
dc.description.abstractLanthanide-doped upconversion nanoparticles (UCNPs) are considered promising novel near-infrared (NIR) bioimaging agents with the characteristics of high contrast and high penetration depth. However, the interactions between charged UCNPs and mammalian cells have not been thoroughly studied, and the corresponding intracellular uptake pathways remain unclear. Herein, our research work involved the use of a hydrothermal method to synthesize polyvinylpyrrolidone-coated UCNPs (UCNP-PVP), and then a ligand exchange reaction was performed on UCNP-PVP, with the help of polyethylenimine (PEI) and poly(acrylic acid) (PAA), to generate UCNP-PEI and UCNP-PAA. These polymer-coated UCNPs demonstrated good dispersibility in aqueous medium, had the same elemental composition and crystal phase, shared similar TEM and dynamic light scattering (DLS) size distribution, and exhibited similar upconversion luminescence efficiency. However, the positively charged UCNP-PEI evinced greatly enhanced cellular uptake in comparison with its neutral or negative counterparts, as shown by multiphoton confocal microscopy and inductively coupled plasma mass spectrometry (ICP-MS) measurements. Meanwhile, we found that cationic UCNP-PEI can be effectively internalized mainly through the clathrin endocytic mechanism, as revealed by colocalization, chemical, and genetic inhibitor studies. This study elucidates the role of the surface polymer coatings in governing UCNP-cell interactions, and it is the first report on the endocytic mechanism of positively charged lanthanide-doped UCNPs. Furthermore, this study provides important guidance for the development of UCNPs as specific intracellular nanoprobes, allowing us to control the UCNP-cell interactions by tuning surface properties.-
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/ancac3/index.html-
dc.relation.ispartofACS NANOen_US
dc.subjectCharge-dependent cellular uptake-
dc.subjectClathrin-mediated endocytosis-
dc.subjectLigand exchange-
dc.subjectUpconversion nanoparticles-
dc.subject.meshErbium - chemistry-
dc.subject.meshFluorides - chemistry-
dc.subject.meshMolecular Imaging - methods-
dc.subject.meshNanoparticles - chemistry - toxicity-
dc.subject.meshPolymers - chemistry - metabolism - toxicity-
dc.titlePolymer-coated NaYF4:Yb3+, Er3+ upconversion nanoparticles for charge-dependent cellular imagingen_US
dc.typeArticleen_US
dc.identifier.emailLee, VHY: h0230446@hkusua.hku.hk-
dc.identifier.emailCheng, SH: bhcheng@cityu.edu.hk-
dc.identifier.emailWong, WT: wtwong@hku.hk-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1021/nn201896m-
dc.identifier.pmid21905691-
dc.identifier.scopuseid_2-s2.0-80054992737-
dc.identifier.hkuros203839en_US
dc.identifier.volume5en_US
dc.identifier.issue10-
dc.identifier.spage7838en_US
dc.identifier.epage7847en_US
dc.identifier.isiWOS:000296208700031-
dc.publisher.placeUnited States-

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