Article: Functional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells

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TitleFunctional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells
AuthorsTong, WY1
Shen, W2
Yeung, CWF2
Zhao, Y2
Cheng, SH2
Chu, PK2
Chan, D1
Chan, GCF1
Cheung, KMC1
Yeung, KWK1
Lam, YW2
KeywordsBioimprinting
Biomimetic material
Mesenchymal stem cells
Tendon
Tissue microenvironment
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
CitationBiomaterials, 2012, v. 33 n. 31, p. 7686-7698 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.biomaterials.2012.07.002
AbstractAlthough many studies have demonstrated that cell phenotype is affected by the surface properties of biomaterials, these materials often fail to mimic the complexity of the native tissue microenvironment (TME). In this study, we have developed a new experimental model that allows the characterisation and functional reconstruction of natural TME. We discovered that mesenchymal stem cells (MSC) cultured on cryostat sections of bovine Achilles tendon adopted an elongated and aligned morphology, and expressed tenocyte marker tenomodulin (TNMD). This suggests that tendon sections contain the signalling cues that guide MSCs to commit to the tenogenic lineage. To reconstruct this instructive niche, we prepared PDMS replica by using tendon sections as template. The resulting bioimprint faithfully copied the physical topography and elasticity of the section. This replica, when coated with collagen 1, supported tenogenesis of MSC without requiring exogenous growth factors. This study illustrates how extracellular biophysical and biochemical features intertwines to form a niche that influences the cell fate and demonstrated that such complex information could be conveniently reconstructed with synthetic materials and purified extracellular matrix proteins.
ISSN0142-9612
2011 Impact Factor: 7.404
2011 SCImago Journal Rankings: 0.633
DOIhttp://dx.doi.org/10.1016/j.biomaterials.2012.07.002
DC Field
Value
dc.contributor.authorTong, WY
dc.contributor.authorShen, W
dc.contributor.authorYeung, CWF
dc.contributor.authorZhao, Y
dc.contributor.authorCheng, SH
dc.contributor.authorChu, PK
dc.contributor.authorChan, D
dc.contributor.authorChan, GCF
dc.contributor.authorCheung, KMC
dc.contributor.authorYeung, KWK
dc.contributor.authorLam, YW
dc.date.accessioned2012-08-16T05:47:56Z
dc.date.available2012-08-16T05:47:56Z
dc.date.issued2012
dc.description.abstractAlthough many studies have demonstrated that cell phenotype is affected by the surface properties of biomaterials, these materials often fail to mimic the complexity of the native tissue microenvironment (TME). In this study, we have developed a new experimental model that allows the characterisation and functional reconstruction of natural TME. We discovered that mesenchymal stem cells (MSC) cultured on cryostat sections of bovine Achilles tendon adopted an elongated and aligned morphology, and expressed tenocyte marker tenomodulin (TNMD). This suggests that tendon sections contain the signalling cues that guide MSCs to commit to the tenogenic lineage. To reconstruct this instructive niche, we prepared PDMS replica by using tendon sections as template. The resulting bioimprint faithfully copied the physical topography and elasticity of the section. This replica, when coated with collagen 1, supported tenogenesis of MSC without requiring exogenous growth factors. This study illustrates how extracellular biophysical and biochemical features intertwines to form a niche that influences the cell fate and demonstrated that such complex information could be conveniently reconstructed with synthetic materials and purified extracellular matrix proteins.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationBiomaterials, 2012, v. 33 n. 31, p. 7686-7698 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.biomaterials.2012.07.002
dc.identifier.citeulike11230396
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2012.07.002
dc.identifier.epage7698
dc.identifier.hkuros204523
dc.identifier.issn0142-9612
2011 Impact Factor: 7.404
2011 SCImago Journal Rankings: 0.633
dc.identifier.issue31
dc.identifier.pmid22818988
dc.identifier.scopuseid_2-s2.0-84865166142
dc.identifier.spage7686
dc.identifier.urihttp://hdl.handle.net/10722/159281
dc.identifier.volume33
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
dc.publisher.placeNetherlands
dc.relation.ispartofBiomaterials
dc.subjectBioimprinting
dc.subjectBiomimetic material
dc.subjectMesenchymal stem cells
dc.subjectTendon
dc.subjectTissue microenvironment
dc.titleFunctional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong