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Article: Functional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells
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TitleFunctional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells
 
AuthorsTong, WY1
Shen, W2
Yeung, CWF2
Zhao, Y2
Cheng, SH2
Chu, PK2
Chan, D1
Chan, GCF1
Cheung, KMC1
Yeung, KWK1
Lam, YW2
 
KeywordsBioimprinting
Biomimetic material
Mesenchymal stem cells
Tendon
Tissue microenvironment
 
Issue Date2012
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
 
CitationBiomaterials, 2012, v. 33 n. 31, p. 7686-7698 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.biomaterials.2012.07.002
 
AbstractAlthough many studies have demonstrated that cell phenotype is affected by the surface properties of biomaterials, these materials often fail to mimic the complexity of the native tissue microenvironment (TME). In this study, we have developed a new experimental model that allows the characterisation and functional reconstruction of natural TME. We discovered that mesenchymal stem cells (MSC) cultured on cryostat sections of bovine Achilles tendon adopted an elongated and aligned morphology, and expressed tenocyte marker tenomodulin (TNMD). This suggests that tendon sections contain the signalling cues that guide MSCs to commit to the tenogenic lineage. To reconstruct this instructive niche, we prepared PDMS replica by using tendon sections as template. The resulting bioimprint faithfully copied the physical topography and elasticity of the section. This replica, when coated with collagen 1, supported tenogenesis of MSC without requiring exogenous growth factors. This study illustrates how extracellular biophysical and biochemical features intertwines to form a niche that influences the cell fate and demonstrated that such complex information could be conveniently reconstructed with synthetic materials and purified extracellular matrix proteins.
 
ISSN0142-9612
2013 Impact Factor: 8.312
 
DOIhttp://dx.doi.org/10.1016/j.biomaterials.2012.07.002
 
DC FieldValue
dc.contributor.authorTong, WY
 
dc.contributor.authorShen, W
 
dc.contributor.authorYeung, CWF
 
dc.contributor.authorZhao, Y
 
dc.contributor.authorCheng, SH
 
dc.contributor.authorChu, PK
 
dc.contributor.authorChan, D
 
dc.contributor.authorChan, GCF
 
dc.contributor.authorCheung, KMC
 
dc.contributor.authorYeung, KWK
 
dc.contributor.authorLam, YW
 
dc.date.accessioned2012-08-16T05:47:56Z
 
dc.date.available2012-08-16T05:47:56Z
 
dc.date.issued2012
 
dc.description.abstractAlthough many studies have demonstrated that cell phenotype is affected by the surface properties of biomaterials, these materials often fail to mimic the complexity of the native tissue microenvironment (TME). In this study, we have developed a new experimental model that allows the characterisation and functional reconstruction of natural TME. We discovered that mesenchymal stem cells (MSC) cultured on cryostat sections of bovine Achilles tendon adopted an elongated and aligned morphology, and expressed tenocyte marker tenomodulin (TNMD). This suggests that tendon sections contain the signalling cues that guide MSCs to commit to the tenogenic lineage. To reconstruct this instructive niche, we prepared PDMS replica by using tendon sections as template. The resulting bioimprint faithfully copied the physical topography and elasticity of the section. This replica, when coated with collagen 1, supported tenogenesis of MSC without requiring exogenous growth factors. This study illustrates how extracellular biophysical and biochemical features intertwines to form a niche that influences the cell fate and demonstrated that such complex information could be conveniently reconstructed with synthetic materials and purified extracellular matrix proteins.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationBiomaterials, 2012, v. 33 n. 31, p. 7686-7698 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.biomaterials.2012.07.002
 
dc.identifier.citeulike11230396
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2012.07.002
 
dc.identifier.eissn1878-5905
 
dc.identifier.epage7698
 
dc.identifier.hkuros204523
 
dc.identifier.issn0142-9612
2013 Impact Factor: 8.312
 
dc.identifier.issue31
 
dc.identifier.pmid22818988
 
dc.identifier.scopuseid_2-s2.0-84865166142
 
dc.identifier.spage7686
 
dc.identifier.urihttp://hdl.handle.net/10722/159281
 
dc.identifier.volume33
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofBiomaterials
 
dc.subjectBioimprinting
 
dc.subjectBiomimetic material
 
dc.subjectMesenchymal stem cells
 
dc.subjectTendon
 
dc.subjectTissue microenvironment
 
dc.titleFunctional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells
 
dc.typeArticle
 
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<item><contributor.author>Tong, WY</contributor.author>
<contributor.author>Shen, W</contributor.author>
<contributor.author>Yeung, CWF</contributor.author>
<contributor.author>Zhao, Y</contributor.author>
<contributor.author>Cheng, SH</contributor.author>
<contributor.author>Chu, PK</contributor.author>
<contributor.author>Chan, D</contributor.author>
<contributor.author>Chan, GCF</contributor.author>
<contributor.author>Cheung, KMC</contributor.author>
<contributor.author>Yeung, KWK</contributor.author>
<contributor.author>Lam, YW</contributor.author>
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<subject>Bioimprinting</subject>
<subject>Biomimetic material</subject>
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Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong