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Article: Functional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells

TitleFunctional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells
Authors
KeywordsBioimprinting
Biomimetic material
Mesenchymal stem cells
Tendon
Tissue microenvironment
Issue Date2012
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2012, v. 33 n. 31, p. 7686-7698 How to Cite?
Abstract
Although many studies have demonstrated that cell phenotype is affected by the surface properties of biomaterials, these materials often fail to mimic the complexity of the native tissue microenvironment (TME). In this study, we have developed a new experimental model that allows the characterisation and functional reconstruction of natural TME. We discovered that mesenchymal stem cells (MSC) cultured on cryostat sections of bovine Achilles tendon adopted an elongated and aligned morphology, and expressed tenocyte marker tenomodulin (TNMD). This suggests that tendon sections contain the signalling cues that guide MSCs to commit to the tenogenic lineage. To reconstruct this instructive niche, we prepared PDMS replica by using tendon sections as template. The resulting bioimprint faithfully copied the physical topography and elasticity of the section. This replica, when coated with collagen 1, supported tenogenesis of MSC without requiring exogenous growth factors. This study illustrates how extracellular biophysical and biochemical features intertwines to form a niche that influences the cell fate and demonstrated that such complex information could be conveniently reconstructed with synthetic materials and purified extracellular matrix proteins.
Persistent Identifierhttp://hdl.handle.net/10722/159281
ISSN
2013 Impact Factor: 8.312
ISI Accession Number ID

 

Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorTong, WYen_HK
dc.contributor.authorShen, Wen_HK
dc.contributor.authorYeung, CWFen_HK
dc.contributor.authorZhao, Yen_HK
dc.contributor.authorCheng, SHen_HK
dc.contributor.authorChu, PKen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorChan, GCFen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorYeung, KWKen_HK
dc.contributor.authorLam, YWen_HK
dc.date.accessioned2012-08-16T05:47:56Z-
dc.date.available2012-08-16T05:47:56Z-
dc.date.issued2012en_HK
dc.identifier.citationBiomaterials, 2012, v. 33 n. 31, p. 7686-7698en_HK
dc.identifier.issn0142-9612en_HK
dc.identifier.urihttp://hdl.handle.net/10722/159281-
dc.description.abstractAlthough many studies have demonstrated that cell phenotype is affected by the surface properties of biomaterials, these materials often fail to mimic the complexity of the native tissue microenvironment (TME). In this study, we have developed a new experimental model that allows the characterisation and functional reconstruction of natural TME. We discovered that mesenchymal stem cells (MSC) cultured on cryostat sections of bovine Achilles tendon adopted an elongated and aligned morphology, and expressed tenocyte marker tenomodulin (TNMD). This suggests that tendon sections contain the signalling cues that guide MSCs to commit to the tenogenic lineage. To reconstruct this instructive niche, we prepared PDMS replica by using tendon sections as template. The resulting bioimprint faithfully copied the physical topography and elasticity of the section. This replica, when coated with collagen 1, supported tenogenesis of MSC without requiring exogenous growth factors. This study illustrates how extracellular biophysical and biochemical features intertwines to form a niche that influences the cell fate and demonstrated that such complex information could be conveniently reconstructed with synthetic materials and purified extracellular matrix proteins.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_HK
dc.relation.ispartofBiomaterialsen_HK
dc.subjectBioimprintingen_HK
dc.subjectBiomimetic materialen_HK
dc.subjectMesenchymal stem cellsen_HK
dc.subjectTendonen_HK
dc.subjectTissue microenvironmenten_HK
dc.titleFunctional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, D: chand@hku.hken_HK
dc.identifier.emailChan, GCF: gcfchan@hku.hken_HK
dc.identifier.emailCheung, KMC: cheungmc@hku.hken_HK
dc.identifier.emailYeung, KWK: wkkyeung@hku.hk-
dc.identifier.emailLam, YW: yunwlam@cityu.edu.hk-
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityYeung, KWK=rp00309en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2012.07.002en_HK
dc.identifier.pmid22818988-
dc.identifier.scopuseid_2-s2.0-84865166142en_HK
dc.identifier.hkuros204523en_US
dc.identifier.volume33-
dc.identifier.issue31-
dc.identifier.spage7686-
dc.identifier.epage7698-
dc.identifier.eissn1878-5905-
dc.identifier.isiWOS:000308619000002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLam, YW=7202563947en_HK
dc.identifier.scopusauthoridYeung, KWK=13309584700en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridChan, GCF=55314229900en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridChu, PK=55313962900en_HK
dc.identifier.scopusauthoridCheng, SH=20233852300en_HK
dc.identifier.scopusauthoridZhao, Y=36982544500en_HK
dc.identifier.scopusauthoridYeung, CWF=55314355700en_HK
dc.identifier.scopusauthoridShen, W=54586058600en_HK
dc.identifier.scopusauthoridTong, WY=37035246400en_HK
dc.identifier.citeulike11230396-

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