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Article: Lycium barbarum extracts protect the brain from blood-brain barrier disruption and cerebral edema in experimental stroke

TitleLycium barbarum extracts protect the brain from blood-brain barrier disruption and cerebral edema in experimental stroke
Authors
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2012, v. 7 n. 3, article no. e33596 How to Cite?
AbstractBACKGROUND AND PURPOSE: Ischemic stroke is a destructive cerebrovascular disease and a leading cause of death. Yet, no ideal neuroprotective agents are available, leaving prevention an attractive alternative. The extracts from the fruits of Lycium barbarum (LBP), a Chinese anti-aging medicine and food supplement, showed neuroprotective function in the retina when given prophylactically. We aim to evaluate the protective effects of LBP pre-treatment in an experimental stroke model. METHODS: C57BL/6N male mice were first fed with either vehicle (PBS) or LBP (1 or 10 mg/kg) daily for 7 days. Mice were then subjected to 2-hour transient middle cerebral artery occlusion (MCAO) by the intraluminal method followed by 22-hour reperfusion upon filament removal. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement, immunohistochemical analysis, and Western blot experiments. Evans blue (EB) extravasation was determined to assess blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP pre-treatment significantly improved neurological deficits as well as decreased infarct size, hemispheric swelling, and water content. Fewer apoptotic cells were identified in LBP-treated brains by TUNEL assay. Reduced EB extravasation, fewer IgG-leaky vessels, and up-regulation of occludin expression were also observed in LBP-treated brains. Moreover, immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were significantly decreased in LBP-treated brains. CONCLUSIONS: Seven-day oral LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin-4 up-regulation, and glial activation. The present study suggests that LBP may be used as a prophylactic neuroprotectant in patients at high risk for ischemic stroke.
Persistent Identifierhttp://hdl.handle.net/10722/159275
ISSN
2014 Impact Factor: 3.234
2014 SCImago Journal Rankings: 1.300
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Azalea Endowment Fund
Funding Information:

This study was supported by the University Development Fund from The University of Hong Kong to the Eye Institute and by the Azalea (1972) Endowment Fund to RCC and KFS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorYang, Den_US
dc.contributor.authorLi, SYen_US
dc.contributor.authorYeung, CMen_US
dc.contributor.authorChang, RCCen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorWong, Den_US
dc.contributor.authorLo, ACYen_US
dc.date.accessioned2012-08-16T05:47:38Z-
dc.date.available2012-08-16T05:47:38Z-
dc.date.issued2012en_US
dc.identifier.citationPLoS One, 2012, v. 7 n. 3, article no. e33596en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/159275-
dc.description.abstractBACKGROUND AND PURPOSE: Ischemic stroke is a destructive cerebrovascular disease and a leading cause of death. Yet, no ideal neuroprotective agents are available, leaving prevention an attractive alternative. The extracts from the fruits of Lycium barbarum (LBP), a Chinese anti-aging medicine and food supplement, showed neuroprotective function in the retina when given prophylactically. We aim to evaluate the protective effects of LBP pre-treatment in an experimental stroke model. METHODS: C57BL/6N male mice were first fed with either vehicle (PBS) or LBP (1 or 10 mg/kg) daily for 7 days. Mice were then subjected to 2-hour transient middle cerebral artery occlusion (MCAO) by the intraluminal method followed by 22-hour reperfusion upon filament removal. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement, immunohistochemical analysis, and Western blot experiments. Evans blue (EB) extravasation was determined to assess blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP pre-treatment significantly improved neurological deficits as well as decreased infarct size, hemispheric swelling, and water content. Fewer apoptotic cells were identified in LBP-treated brains by TUNEL assay. Reduced EB extravasation, fewer IgG-leaky vessels, and up-regulation of occludin expression were also observed in LBP-treated brains. Moreover, immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were significantly decreased in LBP-treated brains. CONCLUSIONS: Seven-day oral LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin-4 up-regulation, and glial activation. The present study suggests that LBP may be used as a prophylactic neuroprotectant in patients at high risk for ischemic stroke.-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBlood-Brain Barrier - drug effects-
dc.subject.meshBrain Edema - etiology - pathology - physiopathology - prevention and control-
dc.subject.meshDrugs, Chinese Herbal - pharmacology-
dc.subject.meshNeuroprotective Agents - pharmacology-
dc.subject.meshStroke - complications - drug therapy - pathology - physiopathology-
dc.titleLycium barbarum extracts protect the brain from blood-brain barrier disruption and cerebral edema in experimental strokeen_US
dc.typeArticleen_US
dc.identifier.emailLi, SY: sukyeeli@hku.hken_US
dc.identifier.emailYeung, CM: ycm1@hku.hken_US
dc.identifier.emailChang, RCC: rccchang@hku.hken_US
dc.identifier.emailSo, KF: hrmaskf@hku.hken_US
dc.identifier.emailWong, D: shdwong@hku.hken_US
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.identifier.authorityWong, DSH=rp00516en_US
dc.identifier.authorityLo, ACY=rp00425en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0033596-
dc.identifier.pmid22438957-
dc.identifier.pmcidPMC3306421-
dc.identifier.scopuseid_2-s2.0-84863346634-
dc.identifier.hkuros205719en_US
dc.identifier.hkuros199715-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863346634&selection=ref&src=s&origin=recordpage-
dc.identifier.volume7en_US
dc.identifier.issue3, article no. e33596-
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000303309100065-
dc.publisher.placeUnited States-
dc.identifier.scopusauthoridYang, D=37662476600-
dc.identifier.scopusauthoridLi, SY=24329630700-
dc.identifier.scopusauthoridYeung, CM=55286822600-
dc.identifier.scopusauthoridChang, RCC=7403713410-
dc.identifier.scopusauthoridSo, KF=34668391300-
dc.identifier.scopusauthoridWong, D=55285624200-
dc.identifier.scopusauthoridLo, ACY=7102780640-

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