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Article: Lycium barbarum extracts protect the brain from blood-brain barrier disruption and cerebral edema in experimental stroke
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TitleLycium barbarum extracts protect the brain from blood-brain barrier disruption and cerebral edema in experimental stroke
 
AuthorsYang, D1
Li, SY1
Yeung, CM1
Chang, RCC1
So, KF1
Wong, D1
Lo, ACY1
 
Issue Date2012
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPLoS One, 2012, v. 7 n. 3, article no. e33596 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0033596
 
AbstractBACKGROUND AND PURPOSE: Ischemic stroke is a destructive cerebrovascular disease and a leading cause of death. Yet, no ideal neuroprotective agents are available, leaving prevention an attractive alternative. The extracts from the fruits of Lycium barbarum (LBP), a Chinese anti-aging medicine and food supplement, showed neuroprotective function in the retina when given prophylactically. We aim to evaluate the protective effects of LBP pre-treatment in an experimental stroke model. METHODS: C57BL/6N male mice were first fed with either vehicle (PBS) or LBP (1 or 10 mg/kg) daily for 7 days. Mice were then subjected to 2-hour transient middle cerebral artery occlusion (MCAO) by the intraluminal method followed by 22-hour reperfusion upon filament removal. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement, immunohistochemical analysis, and Western blot experiments. Evans blue (EB) extravasation was determined to assess blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP pre-treatment significantly improved neurological deficits as well as decreased infarct size, hemispheric swelling, and water content. Fewer apoptotic cells were identified in LBP-treated brains by TUNEL assay. Reduced EB extravasation, fewer IgG-leaky vessels, and up-regulation of occludin expression were also observed in LBP-treated brains. Moreover, immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were significantly decreased in LBP-treated brains. CONCLUSIONS: Seven-day oral LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin-4 up-regulation, and glial activation. The present study suggests that LBP may be used as a prophylactic neuroprotectant in patients at high risk for ischemic stroke.
 
ISSN1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0033596
 
PubMed Central IDPMC3306421
 
ISI Accession Number IDWOS:000303309100065
Funding AgencyGrant Number
University of Hong Kong
Azalea Endowment Fund
Funding Information:

This study was supported by the University Development Fund from The University of Hong Kong to the Eye Institute and by the Azalea (1972) Endowment Fund to RCC and KFS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYang, D
 
dc.contributor.authorLi, SY
 
dc.contributor.authorYeung, CM
 
dc.contributor.authorChang, RCC
 
dc.contributor.authorSo, KF
 
dc.contributor.authorWong, D
 
dc.contributor.authorLo, ACY
 
dc.date.accessioned2012-08-16T05:47:38Z
 
dc.date.available2012-08-16T05:47:38Z
 
dc.date.issued2012
 
dc.description.abstractBACKGROUND AND PURPOSE: Ischemic stroke is a destructive cerebrovascular disease and a leading cause of death. Yet, no ideal neuroprotective agents are available, leaving prevention an attractive alternative. The extracts from the fruits of Lycium barbarum (LBP), a Chinese anti-aging medicine and food supplement, showed neuroprotective function in the retina when given prophylactically. We aim to evaluate the protective effects of LBP pre-treatment in an experimental stroke model. METHODS: C57BL/6N male mice were first fed with either vehicle (PBS) or LBP (1 or 10 mg/kg) daily for 7 days. Mice were then subjected to 2-hour transient middle cerebral artery occlusion (MCAO) by the intraluminal method followed by 22-hour reperfusion upon filament removal. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement, immunohistochemical analysis, and Western blot experiments. Evans blue (EB) extravasation was determined to assess blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP pre-treatment significantly improved neurological deficits as well as decreased infarct size, hemispheric swelling, and water content. Fewer apoptotic cells were identified in LBP-treated brains by TUNEL assay. Reduced EB extravasation, fewer IgG-leaky vessels, and up-regulation of occludin expression were also observed in LBP-treated brains. Moreover, immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were significantly decreased in LBP-treated brains. CONCLUSIONS: Seven-day oral LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin-4 up-regulation, and glial activation. The present study suggests that LBP may be used as a prophylactic neuroprotectant in patients at high risk for ischemic stroke.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPLoS One, 2012, v. 7 n. 3, article no. e33596 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0033596
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0033596
 
dc.identifier.eissn1932-6203
 
dc.identifier.hkuros205719
 
dc.identifier.hkuros199715
 
dc.identifier.isiWOS:000303309100065
Funding AgencyGrant Number
University of Hong Kong
Azalea Endowment Fund
Funding Information:

This study was supported by the University Development Fund from The University of Hong Kong to the Eye Institute and by the Azalea (1972) Endowment Fund to RCC and KFS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

 
dc.identifier.issn1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
dc.identifier.issue3, article no. e33596
 
dc.identifier.pmcidPMC3306421
 
dc.identifier.pmid22438957
 
dc.identifier.scopuseid_2-s2.0-84863346634
 
dc.identifier.urihttp://hdl.handle.net/10722/159275
 
dc.identifier.volume7
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS One
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshBlood-Brain Barrier - drug effects
 
dc.subject.meshBrain Edema - etiology - pathology - physiopathology - prevention and control
 
dc.subject.meshDrugs, Chinese Herbal - pharmacology
 
dc.subject.meshNeuroprotective Agents - pharmacology
 
dc.subject.meshStroke - complications - drug therapy - pathology - physiopathology
 
dc.titleLycium barbarum extracts protect the brain from blood-brain barrier disruption and cerebral edema in experimental stroke
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine