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Article: Effects of sperm DNA damage on the levels of RAD51 and p53 proteins in zygotes and 2-cell embryos sired by golden hamsters without the major accessory sex glands

TitleEffects of sperm DNA damage on the levels of RAD51 and p53 proteins in zygotes and 2-cell embryos sired by golden hamsters without the major accessory sex glands
Authors
Issue Date2012
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed
Citation
Free Radical Biology & Medicine, 2012, v. 53 n. 4, p. 885-892 How to Cite?
AbstractWe previously reported that the male accessory sex gland (ASG) secretion is the main source of antioxidants to safeguard sperm genomic integrity and functional competence. Removal of all ASGs in the golden hamster can reduce male fertility by increasing embryo wastage. This study aims to investigate whether the oxidative DNA-damaged sperm from hamsters without all ASGs (TX) could successfully fertilize oocytes and to qualify the status of DNA repair by the expression of RAD51 and p53 proteins. Here we demonstrated a significantly higher DNA-base adduct formation (8-hydroxy-2'-deoxyguanosine) in sperm from TX males than those from sham-operated males. Comet assays demonstrated that all female pronuclei in both zygotes were intact, but single- and double-strand DNA damage was found in decondensed sperm in TX males only. DNA damage could also be detected in both nuclei of the TX 2-cell embryos. RAD51, a DNA repair enzyme, was found to be evenly distributed in the cytoplasm and nuclei in oocytes/zygotes, while at the 2-cell stage, a strong expression of p53 protein and a larger clear perinuclear area without RAD51 expression were found in TX embryos. In conclusion, we demonstrated for the first time DNA damage in decondensed sperm of zygotes and blastomeres of 2-cell stage embryos sired by TX males, resulting in the activation of DNA repair. Sperm DNA damage could induce the increase in p53 expression and the reduction of RAD51 expression in the TX 2-cell stage embryos.
Persistent Identifierhttp://hdl.handle.net/10722/159273
ISSN
2014 Impact Factor: 5.736
2013 SCImago Journal Rankings: 2.220
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_US
dc.contributor.authorLiao, Sen_US
dc.contributor.authorCheung, MPLen_US
dc.contributor.authorChow, PHen_US
dc.contributor.authorCheung, Aen_US
dc.contributor.authorO, WSen_US
dc.date.accessioned2012-08-16T05:47:37Z-
dc.date.available2012-08-16T05:47:37Z-
dc.date.issued2012en_US
dc.identifier.citationFree Radical Biology & Medicine, 2012, v. 53 n. 4, p. 885-892en_US
dc.identifier.issn0891-5849-
dc.identifier.urihttp://hdl.handle.net/10722/159273-
dc.description.abstractWe previously reported that the male accessory sex gland (ASG) secretion is the main source of antioxidants to safeguard sperm genomic integrity and functional competence. Removal of all ASGs in the golden hamster can reduce male fertility by increasing embryo wastage. This study aims to investigate whether the oxidative DNA-damaged sperm from hamsters without all ASGs (TX) could successfully fertilize oocytes and to qualify the status of DNA repair by the expression of RAD51 and p53 proteins. Here we demonstrated a significantly higher DNA-base adduct formation (8-hydroxy-2'-deoxyguanosine) in sperm from TX males than those from sham-operated males. Comet assays demonstrated that all female pronuclei in both zygotes were intact, but single- and double-strand DNA damage was found in decondensed sperm in TX males only. DNA damage could also be detected in both nuclei of the TX 2-cell embryos. RAD51, a DNA repair enzyme, was found to be evenly distributed in the cytoplasm and nuclei in oocytes/zygotes, while at the 2-cell stage, a strong expression of p53 protein and a larger clear perinuclear area without RAD51 expression were found in TX embryos. In conclusion, we demonstrated for the first time DNA damage in decondensed sperm of zygotes and blastomeres of 2-cell stage embryos sired by TX males, resulting in the activation of DNA repair. Sperm DNA damage could induce the increase in p53 expression and the reduction of RAD51 expression in the TX 2-cell stage embryos.-
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed-
dc.relation.ispartofFree Radical Biology & Medicineen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Free Radical Biology & Medicine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Free Radical Biology & Medicine, [VOL 53, ISSUE 4, 2012] DOI 10.1016/j.freeradbiomed.2012.06.007-
dc.titleEffects of sperm DNA damage on the levels of RAD51 and p53 proteins in zygotes and 2-cell embryos sired by golden hamsters without the major accessory sex glandsen_US
dc.typeArticleen_US
dc.identifier.emailLiao, S: lsb776@hotmail.comen_US
dc.identifier.emailCheung, MPL: mplcheun@hkucc.hku.hken_US
dc.identifier.emailCheung, A: lmcheung@hku.hken_US
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_US
dc.identifier.authorityCheung, A=rp00332en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.freeradbiomed.2012.06.007-
dc.identifier.pmid22705368-
dc.identifier.hkuros205573en_US
dc.identifier.volume53en_US
dc.identifier.issue4-
dc.identifier.spage885en_US
dc.identifier.epage892en_US
dc.identifier.isiWOS:000307920100024-
dc.publisher.placeUnited States-

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