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Article: Mast cells activation contribute to small intestinal ischemia reperfusion induced acute lung injury in rats

TitleMast cells activation contribute to small intestinal ischemia reperfusion induced acute lung injury in rats
Authors
KeywordsMast cell
Degranulation
Intestinal ischemia reperfusion
Lung injury
Issue Date2012
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/injury
Citation
Injury, 2012, v. 43 n. 8, p. 1250-1256 How to Cite?
AbstractBACKGROUND: Small intestinal ischemia-reperfusion (IIR) injury may lead to severe local and remote tissue injury, especially acute lung injury (ALI). Mast cell activation plays an important role in IIR injury. It is unknown whether IIR mediates lung injury via mast cell activation. METHODS: Adult SD rats were randomized into sham operated group (S), sole IIR group (IIR) in which rats were subjected to 75 min of superior mesenteric artery occlusion followed by 4h reperfusion, or IIR being respectively treated with the mast cell stabilizer Cromolyn Sodium (IIR+CS group), with the tryptase antagonist Protamine (IIR+P group), with the histamine receptor antagonist Ketotifen (IIR+K group), or with the mast cell degranulator Compound 48/80 (IIR+CP group). The above agents were, respectively, administrated intravenously 5 min before reperfusion. At the end of experiment, lung tissue was obtained for histologic assessment and assays for protein expressions of tryptase and mast cell protease 7(MCP7). Pulmonary mast cell number and levels of histamine, TNF-alpha and IL-8 were quantified. RESULTS: IIR resulted in lung injury evidenced as significant increases in lung histological scores (P<0.05 IIR vs. S), accompanied with concomitant increases of mast cell counts and elevations in TNF-alpha and IL-8 concentrations and reductions in histamine levels (all P<0.05 IIR vs. S). IIR also increased lung tissue tryptase and MCP7 protein expressions (all P<0.05, IIR vs. S). Cromolyn Sodium, Ketotifen and Protamine significantly reduced whilst Compound 48/80 aggravated IIR mediated ALI and the above biochemical changes (P<0.05). CONCLUSIONS: Mast cells activation play a critical role in IIR mediated ALI.
Persistent Identifierhttp://hdl.handle.net/10722/159259
ISSN
2015 Impact Factor: 1.91
2015 SCImago Journal Rankings: 0.982
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHuang, Pen_US
dc.contributor.authorLiu, Den_US
dc.contributor.authorGan, Xen_US
dc.contributor.authorZhang, Ren_US
dc.contributor.authorGao, Wen_US
dc.contributor.authorXia, Zen_US
dc.contributor.authorHei, Zen_US
dc.date.accessioned2012-08-16T05:47:14Z-
dc.date.available2012-08-16T05:47:14Z-
dc.date.issued2012en_US
dc.identifier.citationInjury, 2012, v. 43 n. 8, p. 1250-1256en_US
dc.identifier.issn0020-1383-
dc.identifier.urihttp://hdl.handle.net/10722/159259-
dc.description.abstractBACKGROUND: Small intestinal ischemia-reperfusion (IIR) injury may lead to severe local and remote tissue injury, especially acute lung injury (ALI). Mast cell activation plays an important role in IIR injury. It is unknown whether IIR mediates lung injury via mast cell activation. METHODS: Adult SD rats were randomized into sham operated group (S), sole IIR group (IIR) in which rats were subjected to 75 min of superior mesenteric artery occlusion followed by 4h reperfusion, or IIR being respectively treated with the mast cell stabilizer Cromolyn Sodium (IIR+CS group), with the tryptase antagonist Protamine (IIR+P group), with the histamine receptor antagonist Ketotifen (IIR+K group), or with the mast cell degranulator Compound 48/80 (IIR+CP group). The above agents were, respectively, administrated intravenously 5 min before reperfusion. At the end of experiment, lung tissue was obtained for histologic assessment and assays for protein expressions of tryptase and mast cell protease 7(MCP7). Pulmonary mast cell number and levels of histamine, TNF-alpha and IL-8 were quantified. RESULTS: IIR resulted in lung injury evidenced as significant increases in lung histological scores (P<0.05 IIR vs. S), accompanied with concomitant increases of mast cell counts and elevations in TNF-alpha and IL-8 concentrations and reductions in histamine levels (all P<0.05 IIR vs. S). IIR also increased lung tissue tryptase and MCP7 protein expressions (all P<0.05, IIR vs. S). Cromolyn Sodium, Ketotifen and Protamine significantly reduced whilst Compound 48/80 aggravated IIR mediated ALI and the above biochemical changes (P<0.05). CONCLUSIONS: Mast cells activation play a critical role in IIR mediated ALI.-
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/injury-
dc.relation.ispartofInjuryen_US
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#-
dc.subjectMast cell-
dc.subjectDegranulation-
dc.subjectIntestinal ischemia reperfusion-
dc.subjectLung injury-
dc.titleMast cells activation contribute to small intestinal ischemia reperfusion induced acute lung injury in ratsen_US
dc.typeArticleen_US
dc.identifier.emailXia, Z: zyxia@hkucc.hku.hken_US
dc.identifier.emailHei, Z: heiziqing@sina.com.cn-
dc.identifier.authorityXia, Z=rp00532en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.injury.2011.12.027-
dc.identifier.pmid22277108-
dc.identifier.scopuseid_2-s2.0-84863534697-
dc.identifier.hkuros204911en_US
dc.identifier.volume43en_US
dc.identifier.issue8en_US
dc.identifier.spage1250en_US
dc.identifier.epage1256en_US
dc.identifier.isiWOS:000306112900004-
dc.publisher.placeUnited Kingdom-
dc.identifier.citeulike10296565-

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