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Article: Allopurinol attenuates left ventricular dysfunction in rats with early stages of streptozotocin-induced diabetes
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TitleAllopurinol attenuates left ventricular dysfunction in rats with early stages of streptozotocin-induced diabetes
 
AuthorsGao, X3
Xu, Y3
Xu, B3
Liu, Y1
Cai, J3
Liu, HM1
Lei, S1
Zhong, YQ2
Irwin, MG1
Xia, Z1
 
Issue Date2012
 
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394
 
CitationDiabetes - Metabolism: Research and Reviews, 2012, v. 28 n. 5, p. 409-417 [How to Cite?]
DOI: http://dx.doi.org/10.1002/dmrr.2295
 
AbstractBACKGROUND: Xanthine oxidase-derived superoxide production and oxidative stress contribute to the development of diabetic complications including diabetic cardiomyopathy. We hypothesized that xanthine oxidase-inhibitor allopurinol (ALP) may decrease hyperglycemia-induced oxidative stress, ameliorate cardiomyocyte hypertrophy and fibrosis, and attenuate the development of left ventricle (LV) diastolic dysfunction in rats with streptozotocin (STZ)-induced diabetes. METHODS: Control Sprague Dawley (C) or streptozotocin-induced diabetic (D) rats were either untreated or treated with allopurinol (100 mg/kg/day) for 4 weeks starting at 1 week after streptozotocin injection. Free 15-F2t-isoprostane, a specific indicator of oxidative stress was measured by enzymatic immunoassay. The cardiomyocyte cross-sectional area was assessed by hematoxylin and eosin-stained paraffin-embedded sections of LVs. Myocardial collagens I and III were assessed by immunol histochemistry and Western blotting. Echocardiography was performed to characterize cardiac structure and function. RESULTS: In diabetic rats, both plasma and cardiac tissue levels of free 15-F2t-isoprostane were increased (p < 0.05 vs. control), accompanied with significant increase (p < 0.05 vs. control) in cross-section area and myocardial collagen deposition of LV cardiomyocyte. Echocardiography in diabetic rats showed that LV weight/body weight ratio was significantly higher than in control rats, whereas the levels of LV end-diastolic volume and stroke volume were decreased (all p < 0.05 diabetic vs. control). All these changes were either attenuated or prevented by allopurinol. In addition, LV ejection fraction in diabetic rats treated with allopurinol was higher than that in untreated diabetic rats (p < 0.05). CONCLUSION: Allopurinol can attenuate hyperglycemia-induced oxidative stress, ameliorate cardiomyocyte hypertrophy and fibrosis and subsequently prevent left ventricular dysfunction in early diabetes. Copyright (c) 2012 John Wiley and Sons, Ltd.
 
ISSN1520-7552
 
DOIhttp://dx.doi.org/10.1002/dmrr.2295
 
ISI Accession Number IDWOS:000306127400005
 
DC FieldValue
dc.contributor.authorGao, X
 
dc.contributor.authorXu, Y
 
dc.contributor.authorXu, B
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorCai, J
 
dc.contributor.authorLiu, HM
 
dc.contributor.authorLei, S
 
dc.contributor.authorZhong, YQ
 
dc.contributor.authorIrwin, MG
 
dc.contributor.authorXia, Z
 
dc.date.accessioned2012-08-16T05:47:13Z
 
dc.date.available2012-08-16T05:47:13Z
 
dc.date.issued2012
 
dc.description.abstractBACKGROUND: Xanthine oxidase-derived superoxide production and oxidative stress contribute to the development of diabetic complications including diabetic cardiomyopathy. We hypothesized that xanthine oxidase-inhibitor allopurinol (ALP) may decrease hyperglycemia-induced oxidative stress, ameliorate cardiomyocyte hypertrophy and fibrosis, and attenuate the development of left ventricle (LV) diastolic dysfunction in rats with streptozotocin (STZ)-induced diabetes. METHODS: Control Sprague Dawley (C) or streptozotocin-induced diabetic (D) rats were either untreated or treated with allopurinol (100 mg/kg/day) for 4 weeks starting at 1 week after streptozotocin injection. Free 15-F2t-isoprostane, a specific indicator of oxidative stress was measured by enzymatic immunoassay. The cardiomyocyte cross-sectional area was assessed by hematoxylin and eosin-stained paraffin-embedded sections of LVs. Myocardial collagens I and III were assessed by immunol histochemistry and Western blotting. Echocardiography was performed to characterize cardiac structure and function. RESULTS: In diabetic rats, both plasma and cardiac tissue levels of free 15-F2t-isoprostane were increased (p < 0.05 vs. control), accompanied with significant increase (p < 0.05 vs. control) in cross-section area and myocardial collagen deposition of LV cardiomyocyte. Echocardiography in diabetic rats showed that LV weight/body weight ratio was significantly higher than in control rats, whereas the levels of LV end-diastolic volume and stroke volume were decreased (all p < 0.05 diabetic vs. control). All these changes were either attenuated or prevented by allopurinol. In addition, LV ejection fraction in diabetic rats treated with allopurinol was higher than that in untreated diabetic rats (p < 0.05). CONCLUSION: Allopurinol can attenuate hyperglycemia-induced oxidative stress, ameliorate cardiomyocyte hypertrophy and fibrosis and subsequently prevent left ventricular dysfunction in early diabetes. Copyright (c) 2012 John Wiley and Sons, Ltd.
 
dc.identifier.citationDiabetes - Metabolism: Research and Reviews, 2012, v. 28 n. 5, p. 409-417 [How to Cite?]
DOI: http://dx.doi.org/10.1002/dmrr.2295
 
dc.identifier.doihttp://dx.doi.org/10.1002/dmrr.2295
 
dc.identifier.epage417
 
dc.identifier.hkuros204903
 
dc.identifier.hkuros203048
 
dc.identifier.isiWOS:000306127400005
 
dc.identifier.issn1520-7552
 
dc.identifier.issue5
 
dc.identifier.pmid22389139
 
dc.identifier.scopuseid_2-s2.0-84863773463
 
dc.identifier.spage409
 
dc.identifier.urihttp://hdl.handle.net/10722/159256
 
dc.identifier.volume28
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofDiabetes - Metabolism: Research and Reviews
 
dc.rightsDiabetes - Metabolism: Research and Reviews. Copyright © John Wiley & Sons Ltd.
 
dc.titleAllopurinol attenuates left ventricular dysfunction in rats with early stages of streptozotocin-induced diabetes
 
dc.typeArticle
 
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<item><contributor.author>Gao, X</contributor.author>
<contributor.author>Xu, Y</contributor.author>
<contributor.author>Xu, B</contributor.author>
<contributor.author>Liu, Y</contributor.author>
<contributor.author>Cai, J</contributor.author>
<contributor.author>Liu, HM</contributor.author>
<contributor.author>Lei, S</contributor.author>
<contributor.author>Zhong, YQ</contributor.author>
<contributor.author>Irwin, MG</contributor.author>
<contributor.author>Xia, Z</contributor.author>
<date.accessioned>2012-08-16T05:47:13Z</date.accessioned>
<date.available>2012-08-16T05:47:13Z</date.available>
<date.issued>2012</date.issued>
<identifier.citation>Diabetes - Metabolism: Research and Reviews, 2012, v. 28 n. 5, p. 409-417</identifier.citation>
<identifier.issn>1520-7552</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/159256</identifier.uri>
<description.abstract>BACKGROUND: Xanthine oxidase-derived superoxide production and oxidative stress contribute to the development of diabetic complications including diabetic cardiomyopathy. We hypothesized that xanthine oxidase-inhibitor allopurinol (ALP) may decrease hyperglycemia-induced oxidative stress, ameliorate cardiomyocyte hypertrophy and fibrosis, and attenuate the development of left ventricle (LV) diastolic dysfunction in rats with streptozotocin (STZ)-induced diabetes. METHODS: Control Sprague Dawley (C) or streptozotocin-induced diabetic (D) rats were either untreated or treated with allopurinol (100 mg/kg/day) for 4 weeks starting at 1 week after streptozotocin injection. Free 15-F2t-isoprostane, a specific indicator of oxidative stress was measured by enzymatic immunoassay. The cardiomyocyte cross-sectional area was assessed by hematoxylin and eosin-stained paraffin-embedded sections of LVs. Myocardial collagens I and III were assessed by immunol histochemistry and Western blotting. Echocardiography was performed to characterize cardiac structure and function. RESULTS: In diabetic rats, both plasma and cardiac tissue levels of free 15-F2t-isoprostane were increased (p &lt; 0.05 vs. control), accompanied with significant increase (p &lt; 0.05 vs. control) in cross-section area and myocardial collagen deposition of LV cardiomyocyte. Echocardiography in diabetic rats showed that LV weight/body weight ratio was significantly higher than in control rats, whereas the levels of LV end-diastolic volume and stroke volume were decreased (all p &lt; 0.05 diabetic vs. control). All these changes were either attenuated or prevented by allopurinol. In addition, LV ejection fraction in diabetic rats treated with allopurinol was higher than that in untreated diabetic rats (p &lt; 0.05). CONCLUSION: Allopurinol can attenuate hyperglycemia-induced oxidative stress, ameliorate cardiomyocyte hypertrophy and fibrosis and subsequently prevent left ventricular dysfunction in early diabetes. Copyright (c) 2012 John Wiley and Sons, Ltd.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Shenzhen Futian District Traditional Chinese Medicine Hospital
  3. Capital Medical University China