File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Lack of association of TIM3 polymorphisms and allergic phenotypes

TitleLack of association of TIM3 polymorphisms and allergic phenotypes
Authors
Issue Date2009
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmedgenet/
Citation
BMC Medical Genetics, 2009, v. 10, article no. 62 How to Cite?
AbstractBACKGROUND: T-cell immunoglobulin mucin-3 (TIM3) is a TH1-specific type 1 membrane protein that regulates TH1 proliferation and the development of immunological tolerance. TIM3 and its genetic variants have been suggested to play a role in regulating allergic diseases. Polymorphisms in the TIM3 promoter region have been reported to be associated with allergic phenotypes in several populations. The aims of this study were to examine whether genetic variation in the promoter region of TIM3 influenced transcription of the gene and risk for allergic phenotypes. METHODS: We performed 5' rapid amplification of cDNA ends and reverse transcription-polymerase chain reaction. We screened for polymorphisms in the promoter region. Deletion analysis was used to localize the promoter region of TIM3. Genotyping was performed by TaqMan assays in three asthma/allergy population samples. RESULTS: We found two regions with promoter activity in TIM3. One region was from -214 bp to +58 bp and the other from -1.6 kb to -914 bp relative to the transcription start site. None of the single nucleotide polymorphisms (SNPs) or haplotypes affected the transcriptional activity in reporter gene assays. No association between the SNPs and any phenotype was observed in the study cohorts. CONCLUSION: Our findings indicate that SNPs and haplotypes in the TIM3 promoter region do not have a functional effect in vitro and are not associated with allergic diseases. These data suggest that polymorphisms in the TIM3 promoter region are unlikely to play an important role in susceptibility to allergic diseases.
Persistent Identifierhttp://hdl.handle.net/10722/159189
ISSN
2015 Impact Factor: 2.094
2015 SCImago Journal Rankings: 1.062
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhang, J-
dc.contributor.authorDaley, D-
dc.contributor.authorAkhabir, L-
dc.contributor.authorStefanowicz, D-
dc.contributor.authorChan-Yeung, M-
dc.contributor.authorBecker, AB-
dc.contributor.authorLaprise, C-
dc.contributor.authorPare, PD-
dc.contributor.authorSandford, AJ-
dc.date.accessioned2012-08-15T06:52:47Z-
dc.date.available2012-08-15T06:52:47Z-
dc.date.issued2009-
dc.identifier.citationBMC Medical Genetics, 2009, v. 10, article no. 62-
dc.identifier.issn1471-2350-
dc.identifier.urihttp://hdl.handle.net/10722/159189-
dc.description.abstractBACKGROUND: T-cell immunoglobulin mucin-3 (TIM3) is a TH1-specific type 1 membrane protein that regulates TH1 proliferation and the development of immunological tolerance. TIM3 and its genetic variants have been suggested to play a role in regulating allergic diseases. Polymorphisms in the TIM3 promoter region have been reported to be associated with allergic phenotypes in several populations. The aims of this study were to examine whether genetic variation in the promoter region of TIM3 influenced transcription of the gene and risk for allergic phenotypes. METHODS: We performed 5' rapid amplification of cDNA ends and reverse transcription-polymerase chain reaction. We screened for polymorphisms in the promoter region. Deletion analysis was used to localize the promoter region of TIM3. Genotyping was performed by TaqMan assays in three asthma/allergy population samples. RESULTS: We found two regions with promoter activity in TIM3. One region was from -214 bp to +58 bp and the other from -1.6 kb to -914 bp relative to the transcription start site. None of the single nucleotide polymorphisms (SNPs) or haplotypes affected the transcriptional activity in reporter gene assays. No association between the SNPs and any phenotype was observed in the study cohorts. CONCLUSION: Our findings indicate that SNPs and haplotypes in the TIM3 promoter region do not have a functional effect in vitro and are not associated with allergic diseases. These data suggest that polymorphisms in the TIM3 promoter region are unlikely to play an important role in susceptibility to allergic diseases.-
dc.languageeng-
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcmedgenet/-
dc.relation.ispartofBMC Medical Genetics-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.mesh5' Flanking Region - genetics-
dc.subject.meshAfrican Continental Ancestry Group-
dc.subject.meshAsthma - genetics-
dc.subject.meshMembrane Proteins - genetics-
dc.subject.meshPolymorphism, Single Nucleotide-
dc.titleLack of association of TIM3 polymorphisms and allergic phenotypesen_US
dc.typeArticleen_US
dc.identifier.emailZhang, J: zjs015195@yahoo.com-
dc.identifier.emailDaley, D: ddaley@mrl.ubc.ca-
dc.identifier.emailAkhabir, L: lakhabir@mrl.ubc.ca-
dc.identifier.emailStefanowicz, D: dstefanowicz@mrl.ubc.ca-
dc.identifier.emailChan-Yeung, M: mmwchan@hku.hk-
dc.identifier.emailBecker, AB: becker@cc.umanitoba.ca-
dc.identifier.emailLaprise, C: Catherine_Laprise@uqac.ca-
dc.identifier.emailPare, PD: ppare@mrl.ubc.ca-
dc.identifier.emailSandford, AJ: asandford@mrl.ubc.ca-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1471-2350-10-62-
dc.identifier.pmid19566956-
dc.identifier.pmcidPMC2711936-
dc.identifier.scopuseid_2-s2.0-67650727928-
dc.identifier.hkuros162553-
dc.identifier.volume10, article no. 62-
dc.identifier.isiWOS:000268635700001-
dc.publisher.placeUnited Kingdom-
dc.identifier.citeulike5028372-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats