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Conference Paper: Detection and enumeration of giardia cysts in river waters of Hong Kong by flocculation-Percoll/sucrose gradient: Immunofluorescence method

TitleDetection and enumeration of giardia cysts in river waters of Hong Kong by flocculation-Percoll/sucrose gradient: Immunofluorescence method
Authors
KeywordsFlocculation
Giardia Cysts
Health Hazard
Immunofluorescence
Parasites
Percoll-Sucrose
River Water
Issue Date1995
PublisherIWA Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst
Citation
The 17th Biennial Conference of the International Association on Water Quality, Budapest, Hungary, 24-30 July 1994. In Water Science and Technology, 1995, v. 31 n. 5-6, p. 431-434 How to Cite?
AbstractWe have established a simple procedure for the enumeration of Giardia cysts in environmental waters of Hong Kong. The procedure involves concentrating water or sewage samples by the flocculation-percoll/sucrose gradient method followed by a modified tube method using immunofluorescent staining containing fluorescein isothiocyanate labelled anti-Giardia monoclonal antibodies. Enumeration of cysts was carried out using fluorescent microscopy. This procedure has the highest recovery rate (61%) compared with other conventional procedures tested (0-4%). This method was successfully applied to the detection and enumeration of Giardia cysts in water samples collected from two selected rivers of Hong Kong, Shing Mun River and Lam Tsuen River. Preliminary results indicate that water of both rivers has high counts of Giardia cysts.
Persistent Identifierhttp://hdl.handle.net/10722/159059
ISSN
2015 Impact Factor: 1.064
2015 SCImago Journal Rankings: 0.469
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHo, BSWen_US
dc.contributor.authorTam, TYen_US
dc.contributor.authorHutton, Pen_US
dc.contributor.authorYam, WCen_US
dc.date.accessioned2012-08-08T09:06:07Z-
dc.date.available2012-08-08T09:06:07Z-
dc.date.issued1995en_US
dc.identifier.citationThe 17th Biennial Conference of the International Association on Water Quality, Budapest, Hungary, 24-30 July 1994. In Water Science and Technology, 1995, v. 31 n. 5-6, p. 431-434en_US
dc.identifier.issn0273-1223en_US
dc.identifier.urihttp://hdl.handle.net/10722/159059-
dc.description.abstractWe have established a simple procedure for the enumeration of Giardia cysts in environmental waters of Hong Kong. The procedure involves concentrating water or sewage samples by the flocculation-percoll/sucrose gradient method followed by a modified tube method using immunofluorescent staining containing fluorescein isothiocyanate labelled anti-Giardia monoclonal antibodies. Enumeration of cysts was carried out using fluorescent microscopy. This procedure has the highest recovery rate (61%) compared with other conventional procedures tested (0-4%). This method was successfully applied to the detection and enumeration of Giardia cysts in water samples collected from two selected rivers of Hong Kong, Shing Mun River and Lam Tsuen River. Preliminary results indicate that water of both rivers has high counts of Giardia cysts.en_US
dc.languageengen_US
dc.publisherIWA Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwsten_US
dc.relation.ispartofWater Science and Technologyen_US
dc.subjectFlocculationen_US
dc.subjectGiardia Cystsen_US
dc.subjectHealth Hazarden_US
dc.subjectImmunofluorescenceen_US
dc.subjectParasitesen_US
dc.subjectPercoll-Sucroseen_US
dc.subjectRiver Wateren_US
dc.titleDetection and enumeration of giardia cysts in river waters of Hong Kong by flocculation-Percoll/sucrose gradient: Immunofluorescence methoden_US
dc.typeConference_Paperen_US
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_US
dc.identifier.authorityYam, WC=rp00313en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0273-1223(95)00306-8en_US
dc.identifier.scopuseid_2-s2.0-0029021695en_US
dc.identifier.volume31en_US
dc.identifier.issue5-6en_US
dc.identifier.spage431en_US
dc.identifier.epage434en_US
dc.identifier.isiWOS:A1995RF87300077-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHo, BSW=7201923025en_US
dc.identifier.scopusauthoridTam, TY=7102012710en_US
dc.identifier.scopusauthoridHutton, P=7006260262en_US
dc.identifier.scopusauthoridYam, WC=7004281720en_US
dc.customcontrol.immutablesml 160610 amended-

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