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Conference Paper: In vitro mechanical and biological assessment of hydroxyapatite-reinforced polyethylene composite

TitleIn vitro mechanical and biological assessment of hydroxyapatite-reinforced polyethylene composite
Authors
Issue Date1997
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530
Citation
Journal Of Materials Science: Materials In Medicine, 1997, v. 8 n. 12, p. 775-779 How to Cite?
AbstractIn vitro performance of hydroxyapatite (HA)-reinforced polyethylene (PE) composite (HAPEX®) has been characterized from both mechanical and biological aspects. The mechanical properties of HAPEX®, such as tensile strength and Young's modulus, showed little change after immersion in a physiological solution at 37 and 70°C for various periods. In addition, the biological response of primary human osteoblast-like (HOB) cells in vitro on HAPEX® was assessed by measuring DNA synthesis and osteoblast phenotype expression. Cell proliferation rate on HAPEX® was demonstrated by an increase in DNA content with time. A high tritiated thymidine ([3H]-TdR) incorporation/DNA rate was observed on day 1 for HAPEX®, indicating a stimulatory effect on cell proliferation. The alkaline phosphatase (ALP) activity was expressed earlier on HAPEX® than on unfilled PE and increased with time, indicating that HOB cells had commenced differentiation. Furthermore, it was found that the HA particles in the composite provided favourable sites for cell attachment. It appears that the presence of HA particles in HAPEX® may have the advantage of acting as microanchors for bone bonding in vivo. | In vitro performance of hydroxyapatite (HA)-reinforced polyethylene (PE) composite (HAPEXTM) has been characterized from both mechanical and biological aspects. The mechanical properties of HAPEXTM, such as tensile strength and Young's modulus, showed little change after immersion in a physiological solution at 37 and 70 °C for various periods. In addition, the biological response of primary human osteoblast-like (HOB) cells in vitro on HAPEXTM was assessed by measuring DNA synthesis and osteoblast phenotype expression. Cell proliferation rate on HAPEXTM was demonstrated by an increase in DNA content with time. A high tritiated thymidine ([3H]-TdR) incorporation/DNA rate was observed on day 1 for HAPEXTM, indicating a stimulatory effect on cell proliferation. The alkaline phosphatase (ALP) activity was expressed earlier on HAPEXTM than on unfilled PE and increased with time, indicating that HOB cells had commenced differentiation. Furthermore, it was found that the HA particles in the composite provided favourable sites for cell attachment. It appears that the presence of HA particles in HAPEXTM may have the advantage of acting as microanchors for bone bonding in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/158920
ISSN
2015 Impact Factor: 2.272
2013 SCImago Journal Rankings: 0.897
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, Jen_US
dc.contributor.authorDi Silvio, Len_US
dc.contributor.authorWang, Men_US
dc.contributor.authorTanner, KEen_US
dc.contributor.authorBonfield, Wen_US
dc.date.accessioned2012-08-08T09:04:35Z-
dc.date.available2012-08-08T09:04:35Z-
dc.date.issued1997en_US
dc.identifier.citationJournal Of Materials Science: Materials In Medicine, 1997, v. 8 n. 12, p. 775-779en_US
dc.identifier.issn0957-4530en_US
dc.identifier.urihttp://hdl.handle.net/10722/158920-
dc.description.abstractIn vitro performance of hydroxyapatite (HA)-reinforced polyethylene (PE) composite (HAPEX®) has been characterized from both mechanical and biological aspects. The mechanical properties of HAPEX®, such as tensile strength and Young's modulus, showed little change after immersion in a physiological solution at 37 and 70°C for various periods. In addition, the biological response of primary human osteoblast-like (HOB) cells in vitro on HAPEX® was assessed by measuring DNA synthesis and osteoblast phenotype expression. Cell proliferation rate on HAPEX® was demonstrated by an increase in DNA content with time. A high tritiated thymidine ([3H]-TdR) incorporation/DNA rate was observed on day 1 for HAPEX®, indicating a stimulatory effect on cell proliferation. The alkaline phosphatase (ALP) activity was expressed earlier on HAPEX® than on unfilled PE and increased with time, indicating that HOB cells had commenced differentiation. Furthermore, it was found that the HA particles in the composite provided favourable sites for cell attachment. It appears that the presence of HA particles in HAPEX® may have the advantage of acting as microanchors for bone bonding in vivo. | In vitro performance of hydroxyapatite (HA)-reinforced polyethylene (PE) composite (HAPEXTM) has been characterized from both mechanical and biological aspects. The mechanical properties of HAPEXTM, such as tensile strength and Young's modulus, showed little change after immersion in a physiological solution at 37 and 70 °C for various periods. In addition, the biological response of primary human osteoblast-like (HOB) cells in vitro on HAPEXTM was assessed by measuring DNA synthesis and osteoblast phenotype expression. Cell proliferation rate on HAPEXTM was demonstrated by an increase in DNA content with time. A high tritiated thymidine ([3H]-TdR) incorporation/DNA rate was observed on day 1 for HAPEXTM, indicating a stimulatory effect on cell proliferation. The alkaline phosphatase (ALP) activity was expressed earlier on HAPEXTM than on unfilled PE and increased with time, indicating that HOB cells had commenced differentiation. Furthermore, it was found that the HA particles in the composite provided favourable sites for cell attachment. It appears that the presence of HA particles in HAPEXTM may have the advantage of acting as microanchors for bone bonding in vivo.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530en_US
dc.relation.ispartofJournal of Materials Science: Materials in Medicineen_US
dc.titleIn vitro mechanical and biological assessment of hydroxyapatite-reinforced polyethylene compositeen_US
dc.typeConference_Paperen_US
dc.identifier.emailWang, M:memwang@hku.hken_US
dc.identifier.authorityWang, M=rp00185en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/A:1018516813604en_US
dc.identifier.scopuseid_2-s2.0-0031452189en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031452189&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume8en_US
dc.identifier.issue12en_US
dc.identifier.spage775en_US
dc.identifier.epage779en_US
dc.identifier.isiWOS:A1997YL60400011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridHuang, J=7408105378en_US
dc.identifier.scopusauthoridDi Silvio, L=7003813975en_US
dc.identifier.scopusauthoridWang, M=15749714100en_US
dc.identifier.scopusauthoridTanner, KE=7005083563en_US
dc.identifier.scopusauthoridBonfield, W=16490765800en_US

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